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FBXW7 (NM_033632) Human Untagged Clone
CAT＃: SC110514
FBXW7 (untagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1
CNY 7,776.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110514 sequence for NM_033632 edited (data generated by NextGen Sequencing)
ATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGA GGTAACCCTTCCTCAAGCCAGGTAGATGAAGAACAGATGAATCGTGTGGTAGAGGAGGAA CAGCAACAGCAACTCAGACAACAAGAGGAGGAGCACACTGCAAGGAATGGTGAAGTTGTT GGAGTAGAACCTAGACCTGGAGGCCAAAATGATTCCCAGCAAGGACAGTTGGAAGAAAAC AATAATAGATTTATTTCGGTAGATGAGGACTCCTCAGGAAACCAAGAAGAACAAGAGGAA GATGAAGAACATGCTGGTGAACAAGATGAGGAGGATGAGGAGGAGGAGGAGATGGACCAG GAGAGTGACGATTTTGATCAGTCTGATGATAGTAGCAGAGAAGATGAACATACACATACT AACAGTGTCACGAACTCCAGTAGTATTGTGGACCTGCCCGTTCACCAACTCTCCTCCCCA TTCTATACAAAAACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAGGTCCGCTCT TTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCACTGGGCTTGTA CCATGTTCAGCAACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAA CAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATG TTTCAGAGCTGGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGT GAACCAACACAAGTAAAACATATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTC ATTTCATTGCTCCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGAC CTGCTACAAGCAGCTCAGACATGTCGCTACTGGAGAATTTTGGCTGAAGACAACCTTCTC TGGAGAGAGAAATGCAAAGAAGAGGGGATTGATGAACCATTGCACATCAAGAGAAGAAAA GTAATAAAACCAGGTTTCATACACAGTCCATGGAAAAGTGCATACATCAGACAGCACAGA ATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGACATGAT GATCATGTGATCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGAC AACACTTTAAAAGTTTGGTCAGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACAT ACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCATCATTAGTGGATCTACAGAT CGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTATACACACCTTATATGGGCAT ACTTCCACTGTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGGTTCTCGAGAT GCCACTCTTAGGGTTTGGGATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCAT GTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGTTAGTGGAGCATATGAT TTTATGGTAAAGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAGGGGCAT ACTAATAGAGTCTATTCATTACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGAT ACATCAATCCGTGTTTGGGATGTGGAGACAGGGAATTGCATTCACACGTTAACAGGGCAC CAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGAT TCTACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATTGCAAGGTCCC AACAAGCATCAGAGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGC TCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTA GTCACATTGGAGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCAAACACA AAGCTGGTGTGTGCAGTTGGGAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTG GACTTTGATGTGGACATGAAGTGA Clone variation with respect to NM_033632.2 >OriGene 5' read for NM_033632 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAAACTTTACAAAAACAAA ATCCGGAGTCTCCCAAACCTGACTGTCCCGGGAGAAGTGGCCCTGGACGGGCAGAAGCCG CAGCCTGAAAAGACCCAGGAAGAGGAAAAGAGGAGTACCGCGCCGGAGCCTTCCGCAGCT GCCGCTTCAGTCCGAAGGAGGAAGGGAACCAACCCACTTTCTCGGCGCCGCGGCTCTTTT CTAAAAGTGATTACTTCCTTAGGATAGATTGCCAGAAGTGGAGTTACTGGGTCAGAGGAA TGTGAAAACCTTTGCATCTTCTGATAGTCTAGCCAAGGTCCAAGAAGTAGCAAGCTGGCT TTTGGAAATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTC TCTGAGAGGTAACCCTTCCTCAAGCCAGGTAGATGAAGAACAGATGAATCGTGTGGTAGA GGAGGAACAGCAACAGCAACTCAGACAACAAGAGGAGGAGCACACTGCAAGGAATGGTGA AGTTGTTGGAGTAGAACCTAGACCTGGAGGCCAAAATGATTCCCAGCAAGGACAGTTGGA AGAAAACAATAATAGATTTATTTCGGTAGATGAGGACTCCTCAGGANACCAAGAAGAACA AGAGGAAGATGAAGAACATGCTGGTGAACAGAATGAGANGATGANGANGNNAGAGANATG GCCANGAGAGTGACNGATTTGATCAGTCTGATGATAGTAGCAGAGAAGATGAACATACAC ATACTAACAGTGTCACGAACTCCAGTAGTATTGTGGACCCTGCCCGTCACAACTNTNCTN NCCATTCTTACAAAACAACAAATGAAAGAAAGTGGACCATGGTNNCTGAGTCCGCTCTTT TTCTTGGAANGAANCATGCAAGTCTAGATATACAAGACACTGGGCTGTACCATGTCACAC ACACACTTTGA >OriGene 3' read for NM_033632 unedited
NGTCATTGGAATGGCACTTCCATGCCAGGTAAAGCACTGGGGNAGGGGTCACAGGGATGC CACCCGGGATCTGTTCAAGAAACAGCTATGACCGCGGCCGCAATCTANAGTCGAGTTTTT TTTTTTTTTTTTTCTTTTTGCAGGGGGAAGGGCAGGGAGTATATCGTCTACACAATTGGA CAAATTCATCTTTTCTGCTCTTCACTTCATGTCCACATCAAAGTCCAGCACCAGCAGCTT GGTTTCTTCAGTCCCATTCCGACTCCCAACTGCACACACCAGCTTTGTGTTTGAGGCTCT GATCCGCCACACAACTCCCCCACTCCCCCCACTCTCCAATGTGACTAGGTTTCGAATAAA TTCACCCGTTTTCAAGTCCCATAGTTTTACAGTTCCATCATCTGAGCTGGTAATTACAAA GTTCTTGTTGAACTGTAAACAGGTCACAGCACTCTGATGCTTGTTGGGACCTTGCAATGT TTGTAAACACTGTCCTGTTTTGATATCCCAGATTTTAACTGTAGAATCTGCATTCCCAGA GACAAGAATATTGTCTTTGAGTTCCATTCCACTTGTTAACGACTGGTGCCCTGTTAACGT GTGAATGCAATTCCCTGTCTCCACATCCCAAACACGGATTGATGTATCAAGAGATCCACT CACCACTTGGAATACCCTCAAACTGTAATGAATAGACTCTATTAATATGCCCCTGCAACG TGTGTAAACAGGTTTCAATTCTCGGGATCCCCCACCTTTACCATAAAATCATATGCTCCA CTTAAAACCCTCCTGCCCTTTTATTGAACCCAGCGGAATGGTGCAACATGACCCATCAAA AAATGTAAACACTGGCCCTGGTCCAATATTCCAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033632 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033632.2, NP_361014.1 |
| RefSeq Size | 3896 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 55294 |
| UniProt ID | Q969H0 |
| Domains | WD40, F-box |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 encode the same isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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FBXW7 and USP7 regulate CCDC6 turnover during the cell cycle and affect cancer drugs susceptibility in NSCLC
,Morra, F;Luise, C;Merolla, F;Poser, I;Visconti, R;Ilardi, G;Paladino, S;Inuzuka, H;Guggino, G;Monaco, R;Colecchia, D;Monaco, G;Cerrato, A;Chiariello, M;Denning, K;Claudio, PP;Staibano, S;Celetti, A;,
Oncotarget
,PubMed ID 25885523
[FBXW7]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217398 | FBXW7 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1 |
CNY 7,768.00 |
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| RC217398L1 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1, Myc-DDK-tagged |
CNY 10,168.00 |
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| RC217398L2 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1, mGFP tagged |
CNY 10,168.00 |
|
| RC217398L3 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1, Myc-DDK-tagged |
CNY 10,168.00 |
|
| RC217398L4 | Lenti ORF clone of Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG217398 | FBXW7 (tGFP-tagged) - Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1 |
CNY 9,368.00 |
