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DNMT3L (NM_175867) Human Untagged Clone
CAT＃: SC110409
DNMT3L (untagged)-Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110409 sequence for NM_175867 edited (data generated by NextGen Sequencing)
ATGGCGGCCATCCCAGCCCTGGACCCAGAGGCCGAGCCCAGCATGGACGTGATTTTGGTG GGATCCAGTGAGCTCTCAAGCTCCGTTTCACCCGGGACAGGCAGAGATCTTATTGCATAT GAAGTCAAGGCTAACCAGCGAAATATAGAAGACATCTGCATCTGCTGCGGAAGTCTCCAG GTTCACACACAGCACCCTCTGTTTGAGGGAGGGATCTGCGCCCCATGTAAGGACAAGTTC CTGGATGCCCTCTTCCTGTACGACGATGACGGGTACCAATCCTACTGCTCCATCTGCTGC TCCGGAGAGACGCTGCTCATCTGCGGAAACCCTGATTGCACCCGATGCTACTGCTTCGAG TGTGTGGATAGCCTGGTCGGCCCCGGGACCTCGGGGAAGGTGCACGCCATGAGCAACTGG GTGTGCTACCTGTGCCTGCCGTCCTCCCGAAGCGGGCTGCTGCAGCGTCGGAGGAAGTGG CGCAGCCAGCTCAAGGCCTTCTACGACCGAGAGTCGGAGAATCCCCTTGAGATGTTCGAA ACCGTGCCTGTGTGGAGGAGACAGCCAGTCCGGGTGCTGTCCCTTTTTGAAGACATCAAG AAAGAGCTGACGAGTTTGGGCTTTTTGGAAAGTGGTTCTGACCCGGGACAACTGAAGCAT GTGGTTGATGTCACAGACACAGTGAGGAAGGATGTGGAGGAGTGGGGACCCTTCGATCTT GTGTACGGCGCCACACCTCCCCTGGGCCACACCTGTGACCGTCCTCCCAGCTGGTACCTG TTCCAGTTCCACCGGCTCCTGCAGTACGCACGGCCCAAGCCAGGCAGCCCCGGGCCCTTC TTCTGGATGTTCGTGGACAATCTGGTGCTGAACAAGGAAGACCTGGACGTCGCATCTCGC TTCCTGGAGATGGAGCCAGTCACCATCCCAGATGTCCACGGCGGATCCTTGCAGAATGCT GTCCGCGTGTGGAGCAACATCCCAGCCATAAGGAGCAGGCACTGGGCTCTGGTTTCGGAA GAAGAATTGTCCCTGCTGGCCCAGAACAAGCAGAGCTCGAAGCTCGCGGCCAAGTGGCCC ACCAAGCTGGTGAAGAACTGCTTTCTCCCCCTAAGAGAATATTTCAAGTATTTTTCAACA GAACTCACTTCCTCTTTATAA Clone variation with respect to NM_175867.1 832 a=>g >OriGene 5' read for NM_175867 unedited
GGAACTTGAGAATTGTAACACCACTTATATAGGCGGCCGCGAATTCGCACGAGGTGGGCC TGGNACCTTCCACTCATCTTTGCCTGCGCCGTCCCTCGTTCCTGGCCCAGTCCTCCCCGG GGACCCTGAGCCTGGAAGCCCCGGACCACTGGAACCTTGAACCCACCAGCTGGCTGTACC CGGAGCCGTGGCAGCAGCCCTCATCCCCATGGCGGCCATCCCAGCCCTGGACCCAGAGGC CGAGCCCAGCATGGACGTGATTTTGGTGGGATCCAGTGAGCTCTCAAGCTCCGTTTCACC CGGGACAGGCAGAGATCTTATTGCATATGAAGTCAAGGCTAACCAGCGAAATATAGAAGA CATCTGCATCTGCTGCGGAAGTCTCCAGGTTCACACACAGCACCCTCTGTTTGAGGGAGG GATCTGCGCCCCATGTAAGGACAAGTTCCTGGATGCCCTCTTCCTGTACGACGATGACGG GTACCAATCCTACTGCTCCATCTGCTGCTCCGGAGAGACGCTGCTCATCTGCGGAAACCC TGATTGCACCCGATGCTACTGCTTCGAGTGTGTGGATAGCCTGGTCGGCCCCGGGACCTC GGGGAAGGTGCACGCCATGAGCAACTGGGTGTGCTACCTGTGCCTGCCGTCCTCCCGAAG CGGGCTGCTGCAGCGTCGGAGGAAGTGGCGCAGCCAGCTCAAGGCCTTCTACGACCGAGA GTCGGAGAATCCCCTTGAGATGTTCGAAACCGTGCCTGTGTGGAGGAGACAGCCAGTCCG GGTGCTGTNCCTTTTTGAAGACATCAAGAAGACTGACGAGTTTGGGCTTTTTGGAAAAGT GGTTCTGACCCGGGACACTGAAGCATGTGGTTGATGTCACANACACGTGAGGAAAGATGT GNA >OriGene 3' read for NM_175867 unedited
CCTTGCGCCATTGGCGATGGTCAACTTGCCAGGGCCAGGAAAGCACTGGGGNAGGGTCAC AGGGCTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATAGTC GAGTTTTTTTTTTTTTTTTTTTTTTTTCTTTGTTCAAAGAAAAGTCTTTTTCTTCACAGT ATAGTGACTCTTTTATAAAGAGGAAGTGAGTTCTGTTGAAAAATACTTGAAATTTTCTCT TAGGGGGAAAAAGCATTTCTTCACCAGCTTGGTGGGCCACTTGGCCGCGAGCTTCGAGCT CTGCTTGTTCTGGGCCAGCAGGGACATTTCTTCTTCCGAAACCAGAGCCCAGTGCCTGCT CCTTATGGCTGGGATGTTGCTCCACACGCGGACAGCATTCTGCAAGGCTCCGCCGTGGAC ATCTGGGATGGTGACTGGCTCCCTCTCCAGGAAGCGAGATGCGACGTCCAGGTCTTCCTT GTTCAGCACCAGATTGTCCACGAACATCCAAAAAAAGGGCCCGGGGCTGCCTGGCTTGGG CCGTGCGTACTGCAGGAGCCGGTGGAACTGGAACAGGTACCAGCTGGGAGGACGGTCACA GGTGTGGCCCAGGGAGAGGTGTGGCGCCGTACACAATATCGAAGGGTCCCCACTCCTCCA CATCCTTCCTCAGTGTGTCTGTGACATCAACCACATGCTTCAGTTGTCCCGGGTCAAATC CACTTTCCAAAAAGCCCAAACTCGTCAGCTCTTTCTTGATGTCTTCAAAAAGGGACAGCA CCCGGACTGGCTGTCTCCTCCCACAGGCACGGTTTCAAACTCTAAGGGGATTCTCGACTC TCGGTCGTAAAGGCCTTGAGCTGGCTGCNCCACTCCTCCGAGCTGCACAGCCCGTTCGGG AGGACG |
| Restriction Sites | Please inquire |
| ACCN | NM_175867 |
| Insert Size | 1300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175867.1, NP_787063.1 |
| RefSeq Size | 1702 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 29947 |
| UniProt ID | Q9UJW3 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at the 3' terminal exon compared to variant 1, which results in an isoform (2) that is 1 aa shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219008 | DNMT3L (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2 |
CNY 3,656.00 |
|
| RC219008L1 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC219008L2 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC219008L3 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219008L4 | Lenti ORF clone of Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG219008 | DNMT3L (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 2 |
CNY 5,256.00 |
