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MED28 (NM_025205) Human Untagged Clone
CAT＃: SC110380
MED28 (untagged)-Human mediator complex subunit 28 (MED28)
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1500003D12Rik; EG1; magicin |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_025205, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCTCCACTAGGGGGTATGTTTTCTGGGCAGCCACCCGGTCCCCCTCAGGCCCCGCCGGGCCTTC CGGGCCAAGCTTCGCTTCTTCAGGCAGCTCCAGGCGCTCCTAGACCTTCCAGCAGTACTTTGGTGGACGA GTTGGAGTCATCTTTCGAGGCTTGCTTTGCATCTCTGGTGAGTCAGGACTATGTCAATGGCACCGATCAG GAAGAAATTCGAACCGGTGTTGATCAGTGTATCCAGAAGTTTCTGGATATTGCAAGACAGACAGAATGTT TTTTCTTACAAAAAAGATTGCAGTTATCTGTCCAGAAACCAGAGCAAGTTATCAAAGAGGATGTGTCAGA ACTAAGGAATGAATTACAGCGGAAAGATGCACTAGTCCAGAAGCACTTGACAAAGCTGAGGCATTGGCAG CAGGTGCTGGAGGACATCAACGTGCAGCACAAAAAGCCCGCCGACATCCCTCAGGGCTCCTTGGCCTACC TGGAGCAGGCATCTGCCAACATCCCTGCACCTCTGAAGCCAACGTGA >OriGene 5' read for NM_025205 unedited
CATTCAGAATTGTAAACGACTCATATAGGCGGCCGCGAATCGGCACGAGCAAACATGGCG GCTCCACTAGGGGGTATGTTTTCTGGGCAGCCACCCGGTCCCCCTCAGGCCCCGCCGGGC CTTCCGGGCCAAGCTTCGCTTCTTCAGGCAGCTCCAGGCGCTCCTAGACCTTCCAGCAGT ACTTTGGTGGACGAGTTGGAGTCATCTTTCGAGGCTTGCTTTGCATCTCTGGTGAGTCAG GACTATGTCAATGGCACCGATCAGGAAGAAATTCGAACCGGTGTTGATCAGTGTATCCAG AAGTTTCTGGATATTGCAAGACAGACAGAATGTTTTTTCTTACAAAAAAGAGTGCAGTTA TCTGTCCAGAAACCAGAGCAAGTTATCAAAGAGGATGTGTCAGAACTAAGGAATGAATTA CAGCGGAAAGATGCACTAGTCCAGAAGCACTTGACACAGCTGAGGCATTGGCAGCAGGTG CTGGAGGACATCAACGTGCAGCACAAAAAAGCCGCCGACATCCCTCAGGGCTCCTTGGCC TACCTGGAGCAGGCATCTGCCAACATCCCTGCACCTCTGAAGCCAACGTGAGCAAAGGGC ACAAGCAGTTGGCCTATGACTGGGCTGATGCCTGAGGTTTGCCCCACATTCCTTTCTGGG GACATGCCTTTTGGCAGGACTCTTTGCCAGAAAATGGAGTCATTTTAGGTTTAGGCCCCC ATGGGAAAATTTCCCCCTTTTTTAAAAGCGGGTAATTTTTTGGGGCCCTTTAACCAAGTC TTGACCTTGGAAAACCCACAAAAGATTTTTTTTTTGGGTTTTACCGCAATTTAAACTGGG AAATTAAAACCCCCAAACTTTTTTAGGGGGCCTTGCTTGG >OriGene 3' read for NM_025205 unedited
NTTTTTGCTCTGGAACCGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTGGC AAAAAAACTTCTGAATTTAATAAAGAAAGGCAACAACTGGTACGTACAGTAATTCAAAAT CTTTTCACTAAGCACCAAATAGGATAGTTCTCAATCTTCTCAAGAACCTCTTTGAAAGAA CGCCTAAAACCTAATCAGTTTATTTAGGTTTTTAGTCAAGAAATACAAAGCTGCCAATCA GAGTTTCTAATTACCATGAGTGACCAGGTTAGCAACCTTGAGGTCATACCACATGAGCTT TTAAAAGCTTTGTTTAACCTATACGTTGTAACTGAGGGGATTTGTTGATGTCAGGCTGGT AATCAGAGATCCATGTGCCAGATACCATATACACACACCAAAAAGATACAGATTATATGG TCCTTATATATTTAAGGAGAATAATGTCTAATTCTCATGGGTGTAGAATTAGTGAGGGGC AGAGAGACAGATACAAAGCACTAACCCACCAGGGAGAACCTAGCGAGGGCAACAGAAGAG ATCAAAGTCCTGTGCCCTGGGGATGCAACTGAATGGGTGCTGGGAGGCTTCAGGGATCAG GCACATGAGTGGATGTCAGTCCATGTGGGAAGGGGCCTTCAAGGCTGAAGACAACAAAAT GAGTCAAGGCACAAAGCCACAGGAGTACTGCCAATCAGAAGACAGTGAAGCATTTGTTCT GGGTAAAGGGATGTGATCTAATAAGATATAAAACTCAGAAAACCTTACCNCATGAGAGCC AGAAATCTCCATTTCTTAACAGCAACTCAACAGGGAAATCTGAGCTGCCCACAAAACTAA CGAGAGCAAAATACCCCTNCCAAGGCAGGGCACCACATNGCTCTTTGTGGAGGCTCAGTA GCCAATGATCACCCTGCAGCCCCTNCCCNACCCCACACGAAACTCTAGTCCCTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_025205 |
| Insert Size | 2350 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025205.3, NP_079481.2 |
| RefSeq Size | 1363 bp |
| RefSeq ORF | 537 bp |
| Locus ID | 80306 |
| UniProt ID | Q9H204 |
| Protein Families | Transcription Factors |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204304 | MED28 (Myc-DDK-tagged)-Human mediator complex subunit 28 (MED28) |
CNY 2,400.00 |
|
| RC204304L3 | Lenti ORF clone of Human mediator complex subunit 28 (MED28), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204304L4 | Lenti ORF clone of Human mediator complex subunit 28 (MED28), mGFP tagged |
CNY 5,890.00 |
|
| RG204304 | MED28 (tGFP-tagged) - Human mediator complex subunit 28 (MED28) |
CNY 4,000.00 |
|
| SC320789 | MED28 (untagged)-Human mediator complex subunit 28 (MED28) |
CNY 2,400.00 |
