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Dicer (DICER1) (NM_030621) Human Untagged Clone
CAT＃: SC110307
DICER1 (untagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 2
CNY 19,384.00
货期*
现货
规格
| Cited in 2 publications. |
Product images
经常一起买 (3)
DICER1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_030621 edited
GAGAGCAGAAGCTTGGGCATGCTGTGATTTTCCAATAAACTGCTATCACAATGTCAAAAT GCAGTTCAGACAAGAGCAACACAGAGATCTCAAACATTAAAACGTAAGCTGTGCTAGAAC AAAAATGCAATGAAAGAAACACTGGATGAATGAAAAGCCCTGCTTTGCAACCCCTCAGCA TGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGC CATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATATCAGGTTGAAC TGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGGAAGA CATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCA GAAATGGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGT CAGCTGTCAGAACTCATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATG CATCTTGGACAAAAGAGAGATGGAACCAAGAGTTTACTAAGCACCAGGTTCTCATTATGA CTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACC TTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCGAGAAATTATGA AGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCTTCCATTTTAA ATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTTA AGAGTAATGCTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCAT GTGAGATTGTGGTGGATTGTGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGC TGATGGAATTAGAAGAAGCACTTAATTTTATCAATGATTGTAATATATCTGTACATTCAA AAGAAAGAGATTCTACTTTAATTTCGAAACAGATACTATCAGACTGTCGTGCCGTATTGG TAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGAATGATGGTAAGAGAACTACAGA AATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTATTGTTTACAGACACTT TCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTTGACCTGA AATTTGTAACTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATG AGCGACAGCAGTTTGAAAGCGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGT CATGGAGTGATTCTGAGGATGATGATGAGGATGAAGAAATTGAAGAAAAAGAGAAGCCAG AGACAAATTTTCCTTCTCCTTTTACCAACATTTTGTGCGGAATTATTTTTGTGGAAAGAA GATACACAGCAGTTGTCTTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGC TGGCTTATATCAGTAGCAATTTCATAACTGGACATGGCATTGGGAAGAATCAGCCTCGCA ACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGGAAATTTCGAGCAC ATGAGACCAACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCAAAAT GCAACTTGGTGGTTCGTTTTGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAG GAAGAGCAAGGGCACCCATCTCTAATTATATAATGTTAGCGGATACAGACAAAATAAAAA GTTTTGAAGAAGACCTTAAAACCTACAAAGCTATTGAAAAGATCTTGAGAAACAAGTGTT CCAAGTCGGTTGATACTGGTGAGACTGACATTGATCCTGTCATGGATGATGATGACGTTT TCCCACCATATGTGTTGAGGCCTGACGATGGTGGTCCACGAGTCACAATCAACACGGCCA TTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGATCCGTTTACTCATCTAGCTC CTAAATGCAGAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTTTATCTGCCAA TTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCTG AAAGAGTTGTAGCTCTCATTTGCTGTGAGAAACTGCACAAAATTGGCGAACTGGATGACC ATTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAGAGGAGCTTGATTTGCATGATG AAGAAGAGACCAGTGTTCCAGGAAGACCAGGTTCCACGAAACGAAGGCAGTGCTACCCAA AAGCAATTCCAGAGTGTTTGAGGGATAGTTATCCCAGACCTGATCAGCCCTGTTACCTGT ATGTGATAGGAATGGTTTTAACTACACCTTTACCTGATGAACTCAACTTTAGAAGGCGGA AGCTCTATCCTCCTGAAGATACCACAAGATGCTTTGGAATACTGACGGCCAAACCCATAC CTCAGATTCCACACTTTCCTGTGTACACACGCTCTGGAGAGGTTACCATATCCATTGAGT TGAAGAAGTCTGGTTTCATGTTGTCTCTACAAATGCTTGAGTTGATTACAAGACTTCACC AGTATATATTCTCACATATTCTTCGGCTTGAAAAACCTGCACTAGAATTTAAACCTACAG ACGCTGATTCAGCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCAGCACTTTGG ATATTGACTTTAAATTCATGGAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCCA GTACAAAGTATACAAAAGAAACACCCTTTGTTTTTAAATTAGAAGATTACCAAGATGCCG TTATCATTCCAAGATATCGCAATTTTGATCAGCCTCATCGATTTTATGTAGCTGATGTGT ACACTGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAGTATGAAACTTTTGCAGAAT ATTATAAAACAAAGTACAACCTTGACCTAACCAATCTCAACCAGCCACTGCTGGATGTGG ACCACACATCTTCAAGACTTAATCTTTTGACACCTCGACATTTGAATCAGAAGGGGAAAG CGCTTCCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCTGCAGAATAAAC AGATACTGGTTCCAGAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAAG CTGTTTGTCTCCCCAGCATACTTTATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAA GAGCCCAGACTGCCAGCGATGCTGGCGTGGGAGTCAGATCACTTCCTGCGGATTTTAGAT ACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGACAGCAAATCTTTCATCTCAATTT CTAACTCCTCTTCAGCTGAAAATGATAATTACTGTAAGCACAGCACAATTGTCCCTGAAA ATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAGAAAATCATGACCAAATGTCTG TGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGAAGTTTCAGCAG ATCTTACAGCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGATT TAGCTAACAGAGACTTTTGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCG TGCAACCAACTACCTCATATTCCATTCAGAATTTATACAGTTACGAGAACCAGCCCCAGC CCAGCGATGAATGTACTCTCCTGAGTAATAAATACCTTGATGGAAATGCTAACAAATCTA CCTCAGATGGAAGTCCTGTGATGGCCGTAATGCCTGGTACGACAGACACTATTCAAGTGC TCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTGGGTACTCCTCAAGGACTCTTG GCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAGTGATGGATTTA ACCTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATATC TATTTTGCACTTACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGG TCAGCAACTGTAATCTGTATCGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGG TGTCAATATTTGATCCCCCTGTGAATTGGCTTCCTCCTGGTTATGTAGTAAATCAAGACA AAAGCAACACAGATAAATGGGAAAAAGATGAAATGACAAAAGACTGCATGCTGGCGAATG GCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGGAGGAGAGCCTGATGTGGAGGG CTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCAGGAACATATCA GATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCTC CTTTTTCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTA GTATGCCATTTTCATCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCT ATCTGGATCCTAGCAAAGCTGTTGAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCAT CAGAAGAAAACTGTGGTGTTGACACGGGAAAGCAGTCCATTTCTTACGACTTGCACACTG AGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGGAAGCCCTGCTGGGCTGCTATT TAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGGGCTGAAGGTGC TCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTCA ACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCT CTTCTGTATTGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTG ATCATCCAGATGCAGATAAAACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAA AGAAAATCAACTACAGATTCAAGAATAAGGCTTACCTTCTCCAGGCTTTTACACATGCCT CCTACCACTACAATACTATCACTGATTGTTACCAGCGCTTAGAATTCCTGGGAGATGCGA TTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGGCAGCACTCCCCGGGGG TCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTGGCTGTAA AGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATG ACTTTGTGCAGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGA GATCTGAGGAGGATGAAGAGAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATA TTTTTGAGTCGCTTGCTGGTGCCATTTACATGGATAGTGGGATGTCACTGGAGACAGTCT GGCAGGTGTACTATCCCATGATGCGGCCACTAATAGAAAAGTTTTCTGCAAATGTACCCC GTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAAACTGCCAAATTTAGCCCGGCTG AGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGAAAGGGGAAATTTA AAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTCCGAA GCCTCAAAGCTAATCAACCTCAGGTTCCCAATAGCTGAAACCGCTTTTTAAAATTCAAAA CA >OriGene 5' read for NM_030621 unedited
GCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNATTCGGCACGAGCCGTGCTG CAGGTTACCTAGGGTATGAATTAATACAGACTTGGAAACTCTGAAAGAACTTAGAATCAG CATTTTGAGAGCAGAAGCTTGGGCATGCTGTGATTTTCCAATAAACTGCTATCACAATGT CAAAATGCAGTTCAGACAAGAGCAACACAGAGATCTCAAACATTAAAACGTAAGCTGTGC TAGAACAAAAATGCAATGAAAGAAACACTGGATGAATGAAAAGCCCTGCTTTGCAACCCC TCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTG GACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATATCAGG TTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAG GGAAGACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACT TCAGCAGAAATGGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAAC AAGTGTCAGCTGTCAGAACTCATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAG TAAATGCATCTTGGACAAAAGAGAGATGGAACCAAGAGTTTACTAAGCACCAGGTTCTCA TTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACTTATCACTGTCAGACA TTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCGAGAAA TTATGAAGCTCTGTGAAAATGTCCATCATGTCCTCGCATTTTGGGACTAAACTGCTTCAT TNTAATGGGAAATGTGATCCAGAGGAATTGGNAGANAGATCAGAACTAA >OriGene 3' read for NM_030621 unedited
TCTGTCACGCGGCCGCATCTATGATCGGTTTTTTTCTCTTTTTTTTTCCTTTCCAAGAGG AAATATGAGCTAGTTTATCTAACTCAATGACCTAAATCACAGATGTATCAAAATTACGGC AGTTTATCGCAAACGTTAAACTTTCACGGCATTAACAGCACAGCTTTGGTAGGTGAAATA TGCATGCAACATTACTGAAAAGTATAGCAAGAAATTTATGTGAGGAGACTAAGGGTAAAG GTGCTGTGTTTTGCTTCTTAAATAAGTAACCAATCAATTATAAAATCTGCAGCATATTTT AGGTGTGATATGCCTAAGGTTTATTTTGTTAGAGCAACAGCCTATAAGGCAAAATTATTT GCCATAAACATTGCCATCAGTGGGAAACTACCTGGCAGGAGGACTTTAGTAAAATGGGGC GCTTAGCCAAAGATCATTTTTATGATAAAAAATATCCGCAGACTACATATTGCTGGGTTT TAAAATGCCTTCAGCATACATATGACAGAAATTTTAGCCATGATCCCTGCAATAAGCTCA CACTATACTAGTGAGTCGCAAGCATCACGCTGCCTCAACGCGCAATGTATAACACCACGA AAGGATTGCATCACCAGCTCGCCCCAACAACATCACAGCTCAAAGACTATTCATGAAGGC CCCACTTTTAAGGCCAGCACTCCTTCTTATGCCAGACTATGCGGTCTCCACACACCTACA ATGCGATTCCCTCACCATGTTCGCTCATCGCCCACTCACACCCCTGTCCCTTTACTATTA GTAGTCGCCGCTCCCGCCCACACTCTTCTCCACCCGCTTCCCCGCCCGAAGCAAACCCTC TCCCGCCCGCCTCTCCCCCCGCGACCTCTCCCGCACCTATCGGCCCACAACTTCGCGGCT CCGCACCCTCCGCCACCCCGCCGTCAGCGGCACACTNTACCCCCTNCTCGTCCACCGCTT GTGCTCCGCTCTCCCCCTACTCACACCCACCTCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_030621 |
| Insert Size | 7500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030621.2, NP_085124.2 |
| RefSeq Size | 10220 bp |
| RefSeq ORF | 5769 bp |
| Locus ID | 23405 |
| UniProt ID | Q9UPY3 |
| Domains | RIBOc, DSRM, DEAD, helicase_C, PAZ, DUF283 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010] Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, 4 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human Dicer Binds Short Single-strand and Double-strand RNA with High Affinity and Interacts with Different Regions of the Nucleic Acids
,Walt F. Lima, Heather Murray, Josh G. Nichols, Hongjiang Wu, Hong Sun, Thazha P. Prakash, Andres R. Berdeja, Hans J. Gaus, and Stanley T. Crooke,
J. Biol. Chem., Jan 2009; 284: 2535 - 2548.
[DICER1]
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Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells
,Timothy A Vickers, Walt F Lima, Josh G Nichols, and Stanley T Crooke,
Nucleic Acids Res. 2007;35(19):6598-610.
[DICER1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211804 | DICER1 (Myc-DDK-tagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 2 |
CNY 19,376.00 |
|
| RC211804L1 | Lenti ORF clone of Human dicer 1, ribonuclease type III (DICER1), transcript variant 2, Myc-DDK-tagged |
CNY 16,060.00 |
|
| RG211804 | DICER1 (tGFP-tagged) - Human dicer 1, ribonuclease type III (DICER1), transcript variant 2 |
CNY 20,976.00 |
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