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EDEM3 (NM_025191) Human Untagged Clone
CAT＃: SC110291
EDEM3 (untagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3)
CNY 6,520.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C1orf22 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110291 sequence for NM_025191 edited (data generated by NextGen Sequencing)
ATGAGCGAAGCCGGCGGCCGGGGCTGTGGGTCCCCGGTTCCCCAGCGAGCGCGATGGAGA CTAGTGGCGGCGACGGCCGCGTTCTGCCTGGTGTCGGCCACCTCCGTGTGGACGGCGGGG GCCGAGCCCATGAGTAGGGAGGAGAAACAGAAGCTTGGGAATCAAGTACTGGAAATGTTT GATCATGCTTATGGTAACTATATGGAACATGCTTACCCTGCTGATGAACTCATGCCTTTA ACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAGTCGCGGTGACGTTGATGATGCCTTG GGAAAATTTTCTCTGACACTGATTGATTCTTTGGACACTCTTGTGGTTTTAAATAAAACT AAAGAATTTGAAGATGCAGTGAGAAAAGTTTTAAGAGATGTTAATTTAGATAACGATGTA GTCGTATCAGTCTTTGAAACAAACATCAGAGTTCTTGGGGGTCTTTTGGGTGGGCACTCC CTGGCAATCATGCTGAAAGAAAAAGGTGAATATATGCAGTGGTACAATGATGAACTTCTC CAAATGGCAAAGCAGTTAGGTTACAAACTTTTACCGGCTTTCAACACTACCAGTGGCCTT CCTTATCCAAGAATTAATTTAAAGTTTGGCATCAGAAAACCAGAAGCTCGGACAGGAACT GAGACAGATACCTGTACAGCTTGTGCAGGTACCTTGATCCTTGAATTTGCTGCTTTAAGT CGATTCACAGGAGCAACAATATTTGAGGAATATGCCAGAAAAGCTCTTGATTTTCTCTGG GAAAAAAGACAGCGAAGTAGTAATTTAGTGGGCGTGACTATAAATATTCATACTGGAGAT TGGGTACGAAAAGATAGTGGAGTTGGAGCAGGGATTGATTCATATTATGAATATCTGTTG AAAGCCTATGTCTTGCTTGGAGATGACAGTTTTCTGGAAAGATTTAACACACACTATGAT GCCATAATGAGGTATATTAGCCAGCCACCTCTTCTACTTGATGTGCATATCCACAAACCA ATGCTGAATGCTCGGACTTGGATGGATGCTTTGCTTGCCTTCTTCCCAGGCTTGCAGGTG TTAAAGGGGGATATTAGACCTGCTATTGAAACTCATGAAATGTTATATCAGGTGATTAAA AAACACATTTTTCTACCAGAGGCATTTACCACAGATTTCAGAGTACACTGGGCTCAACAT CCTTTAAGGCCAGAATTTGCAGAAAGTACCTACTTCTTATATAAAGCTACAGGAGATCCT TACTACCTTGAAGTAGGGAAGACACTTATTGAAAATTTAAATAAATATGCTAGAGTGCCT TGCGGATTTGCTGCCATGAAGGATGTTCGTACTGGAAGTCATGAGGACAGAATGGATTCT TTCTTCTTGGCTGAAATGTTTAAATATCTTTACCTGTTATTTGCTGATAAAGAAGACATT ATTTTTGACATAGAAGATTACATCTTTACAACAGAAGCTCATCTGTTACCTCTTTGGCTC TCTACTACAAATCAAAGCATCTCTAAAAAGAACACAACCTCGGAATATACAGAACTGGAT GACAGTAACTTCGATTGGACTTGTCCAAATACTCAGATCCTCTTTCCTAATGACCCATTG TATGCTCAAAGTATTCGTGAGCCCTTGAAAAATGTGGTGGATAAGAGCTGTCCTAGAGGC ATCATCAGAGTAGAGGAGAGTTTCAGGAGTGGAGCTAAACCCCCTCTGAGAGCCAGAGAT TTCATGGCCACTAACCCTGAGCATTTAGAAATCCTGAAGAAGATGGGGGTGAGTTTGATT CACCTCAAAGATGGGAGAGTCCAGTTGGTCCAACATGCAATCCAAGCTGCTAGTTCAATC GACGCTGAAGATGGGTTGAGGTTCATGCAGGAGATGATTGAATTGTCAAGTCAGCAACAA AAAGAACAGCAGCTGCCTCCACGAGCTGTACAAATTGTTTCCCACCCATTTTTTGGCAGG GTAGTATTGACTGCTGGACCAGCTCAGTTTGGGCTGGATCTGTCTAAACATAAAGAGACA AGAGGATTTGTTGCAAGCAGTAAACCATCCAATGGTTGTTCAGAGCTTACTAACCCAGAG GCAGTGATGGGAAAAATCGCACTGATACAAAGAGGACAGTGCATGTTTGCAGAAAAGGCA CGCAACATCCAGAATGCTGGAGCCATTGGTGGCATTGTTATTGATGACAATGAGGGGAGC AGCAGTGATACTGCCCCTCTGTTCCAGATGGCAGGTGATGGAAAGGATACAGATGACATC AAGATCCCCATGCTGTTCTTATTCAGCAAAGAAGGAAGTATCATACTGGATGCCATCCGG GAATATGAGGAGGTAGAAGTGCTCCTCTCTGATAAAGCAAAAGATCGAGATCCTGAAATG GAAAATGAAGAACAACCATCCTCTGAAAATGATTCTCAGAATCAGAGTGGTGAACAGATT TCATCAAGTTCTCAGGAGGTTGATTTGGTTGATCAAGAGTCTTCTGAGGAAAATTCTCTA AATTCTCACCCAGAATCATTATCTCTAGCAGATATGGACAATGCTGCAAGCATTTCCCCT TCTGAACAGACTTCTAATCCCACAGAAAACCATGAGACTACAAATCTTAATGGTGAATGT ACAGATTTAGATAACCAGCTTCAAGAACAATCAGAAACTGAGGAAGATTCCAATCCTAAT GTTAGCTGGGGTAAAAAGGTCCAGCCTATAGACTCCATATTAGCAGACTGGAATGAAGAT ATAGAAGCATTTGAAATGATGGAGAAGGATGAGCTATGA Clone variation with respect to NM_025191.3 1148 a=>t >OriGene 5' read for NM_025191 unedited
GGTGTTCTAANATTTAGGTNATCACGNACTTACTTTTAGGGNCGGNNCGNGTATTTGGNA CGAGGGCCGGNGTGCCGTTCGTGTTACTCCGCATCCCGCCCCGTCTCGGCACGGCTAGCA GCCCCCTGGCCACCAGCGTCCAGCAATGTGTCTCACCGGCCGGGCGTAGCAGCTGCGCGT GCGCGGAACCGCGGGGCCATGAGCGAAGCCGGCGGCCGGGGCTGTGGGTCCCCGGTTCCC CAGCGAGCGCGATGGAGACTAGTGGCGGCGACGGCCGCGTTCTGCCTGGTGTCGGCCACC TCCGTGTGGACGGCGGGGGCCGAGCCCATGAGTAGGGAGGAGAAACAGAAGCTTGGGAAT CAAGTACTGGAAATGTTTGATCATGCTTATGGTAACTATATGGAACATGCTTACCCTGCT GATGAACTCATGCCTTTAACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAGTCGCGGC GACGTTGATGATGCCTTGGGAAAATTTTCTCTGACACCTGATTGATTCTTTGGACACTCT TGCGGTTTTAAATAACACTAAAGAATCTCGATATGCCCTGAGAAAAGTTTTAAGAGATGT TAATTTACATAACGATGCACTCGTATCAGTCCTTTGTAACCAACATTATTATTTCTTGGG GGTCTTTTTGGGTGGGCACTCCCTGCGCATTATGCTGAAAGAAAAGGTGTATATATGCAG CGGTACAATGATGAACTTTTTCCAAAGGGCAAGCAGTTTTGTTACAACTTTTTACCGGCT TTTAACATTACCAGTGGCCTTTCTTATCCACGATTAATTAAAAGTTTGCCTTAACAAACC ACAATCTTGGCCGTAACTCGAAAGATTCCTGTACAGCTGTGCAGAACCTTGATCCTTGAT TCGTTGTTTAATCTTTTTCAGGACCACCTATTTCGAGAACTTGG >OriGene 3' read for NM_025191 unedited
CGAGTGACCGAGGCCGCGCTCGAGTATACGAGTAAATCCCTTTTTTTTTTAAGGATTTAC TCCTCCTTAACAAGTGAACAATTTGCTTCTAACCGTCAATGAAAGGCAACACCTCCCTCT AATGGCCAAAGGAAGAGAGTGGCAGTAAGCTGGCTTTTCCAATGTGCCACACAATCCTTC ATGCCATTAACTTCTCCTTGCTGGAAAAGAAATCAGGTTGTCTCGATCACCTCACAAAAG TCACTCCTACACAACACCGACTTTCACCTACAAATACACCCTCCCATCCATGCCTATACC GCTCCCGCCCCCCATGCCCTACAGGCACCATCCGCTCATTCTCCTCCCGCCTCCCCCCCC TCTCCCACCGTCCGCCCTGTCTTTTATCTCCGACTATCCCGCCCCCCCCTCGCCTCCCCC TCCCCTCCCCCCCTTCTCCCCTCCCGCCTNTCCCCCCCCCCCCCCCCCCCCACCTCTCAT CCCTTCCTCTTCACCNCCTTCCCCCCTCTTCTCTCCCCCCTTCCCGCCCCCCCCCCCCTC CGCTCCCCCTCTCTCTTCCCCTCTCCTCCCTCTCCGCCCTCCCACCTCCCCCCGCGCCCT TCTCCTCCCCCCCTCCCCTCCCCACCCCTCCCCCCGCCCCTCCCTCTCCCCCCCCCCCCC CCGTCCTCTTCCCCCCCCCCACCCTCCCCCCCCCCCCCCCCCCCTCTTTCCCCCCCCCCC CCTTCCCTCCCCCCCTTCCCCCTCCCCTCCCCCCCTCCCCCCCCCCCCCCCCCACCCCTC ATCCCCCCCCCCCCCCCCCCCCCCTACTCCCTCTCCCCTTCCCCTACTCCTCGCCCCACC CCCTCCCCCCCCCCCTCACCCTCCCCCCACCCCCCCTCCCCACCTCCTCCACCCCCCCTC TCCCCCTTCCCTGCCCCCCCACGTCCTAACCCTCACCCCCCCCCAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_025191 |
| Insert Size | 5410 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025191.2, NP_079467.2 |
| RefSeq Size | 6298 bp |
| RefSeq ORF | 2670 bp |
| Locus ID | 80267 |
| UniProt ID | Q9BZQ6 |
| Domains | Glyco_hydro_47, PA |
| Gene Summary | Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222885 | EDEM3 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 6,824.00 |
|
| RC222885L1 | Lenti-ORF clone of EDEM3 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 9,224.00 |
|
| RC222885L2 | Lenti-ORF clone of EDEM3 (mGFP-tagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 9,030.00 |
|
| RC222885L3 | Lenti-ORF clone of EDEM3 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 9,030.00 |
|
| RC222885L4 | Lenti-ORF clone of EDEM3 (mGFP-tagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 9,030.00 |
|
| RG222885 | EDEM3 (tGFP-tagged) - Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 7,890.00 |
|
| SC318399 | EDEM3 (untagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3) |
CNY 18,050.00 |
