DHX30 (NM_014966) Human Untagged Clone
CAT#: SC110281
DHX30 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2
CNY 8,048.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DDX30; NEDMIAL; RETCOR |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_014966, the custom clone sequence may differ by one or more nucleotides
ATGGCAGCTTCTAGGGACCTATTAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAA GAGCCCTCGGCATCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAA AGTCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGATCGATGCT GAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGCTAGGTCCCCGGAATGAGT TGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTGGCTCCCCTGCCGACAGCTGGTGGCGTCC GGAACCCACCATGCCCCCTACTTCCTGGCGGCAGCTGAATCCAGAGAGTATTCGACCAGGGGGACCTGGG GGCCTATCCCGCTCTTTAGGCCGGGAAGAAGAGGAGGACGAGGAGGAAGAGCTAGAAGAAGGGACCATAG ATGTTACCGACTTCTTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGGAGTTC CTTTGAGATGACAGATGACGACAGTGCCATTAGGGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTG GCCAAGGTGATTCAGATTGCAACGTCATCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGCA CCAAGACCAAGCTGTCTACACTCACCCTGCTCTGGCCCTGCCCCATGACCTTTGTTGCCAAAGGGCGCCG CAAAGCAGAGGCTGAGAATAAGGCGGCAGCCTTGGCCTGCAAGAAACTGAAGAGCCTGGGCCTGGTGGAC AGGAACAACGAACCGCTTACACACGCCATGTATAACCTGGCCTCTTTGCGTGAGCTGGGTGAGACCCAGC GCCGACCATGCACCATCCAGGTGCCCGAGCCCATCCTCCGCAAGATAGAGACCTTCCTGAACCATTACCC TGTGGAGAGTTCATGGATCGCCCCAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTGGGCAAGGAC TCAGGGCCTCTGAGTGACCCTATCACAGGCAAGCCCTATGTGCCCCTGTTGGAAGCAGAGGAGGTACGTC TCAGCCAGAGTCTGCTAGAACTGTGGCGGCGGCGAGGGCCGGTCTGGCAGGAGGCCCCCCAGCTACCTGT GGACCCACATCGGGACACCATCCTCAACGCCATTGAGCAGCACCCGGTGGTGGTCATCTCTGGGGACACG GGCTGTGGGAAGACCACGCGCATCCCCCAGCTGTTGCTGGAGCGCTATGTGACCGAGGGCCGAGGTGCCC GCTGCAATGTTATCATCACCCAACCTCGCCGCATCTCTGCTGTGTCTGTGGCACAGCGGGTCAGCCACGA ACTGGGCCCCTCCCTGCGCCGGAATGTGGGCTTCCAGGTGCGGTTGGAAAGTAAGCCCCCATCCCGAGGC GGGGCCCTGCTCTTCTGCACTGTGGGTATCCTGCTGCGTAAGCTGCAGAGCAACCCCAGCCTGGAGGGCG TGAGCCACGTCATCGTGGATGAGGTGCATGAGCGGGACGTGAACACAGACTTTCTGCTGATCCTGCTCAA GGGCCTGCAGCGGCTCAACCCGGCCCTGCGGCTGGTGCTCATGAGTGCCACAGGGGACAATGAGCGCTTC TCCCGATACTTTGGTGGCTGCCCCGTCATCAAGGTGCCTGGCTTCATGTACCCAGTCAAGGAGCACTACC TAGAGGACATCCTGGCCAAGTTGGGCAAGCACCAGTACCTGCACCGGCACCGGCACCATGAGTCTGAGGA TGAATGCGCACTCGATTTGGACCTTGTGACTGATCTGGTTCTGCACATCGATGCTCGCGGGGAACCAGGT GGGATCCTGTGCTTCCTGCCTGGGTGGCAGGAGATCAAAGGAGTGCAGCAGCGCCTCCAGGAGGCCCTGG GCATGCACGAGAGCAAGTACCTCATCCTGCCAGTGCACTCCAACATCCCCATGATGGATCAGAAGGCCAT ATTCCAGCAGCCTCCAGTTGGGGTGCGCAAGATTGTCTTGGCCACCAACATTGCTGAGACTTCCATCACA ATCAATGACATCGTGCATGTGGTGGACAGTGGGCTGCACAAGGAAGAACGCTATGACCTGAAGACCAAGG TGTCCTGCCTGGAGACAGTGTGGGTATCAAGAGCCAATGTGATCCAGCGCCGGGGCCGGGCGGGCCGCTG CCAGTCCGGCTTTGCCTACCACTTGTTCCCTCGAAGCCGGCTGGAGAAAATGGTCCCTTTCCAAGTGCCA GAGATCCTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCGAAAATCCACATGCCTGAGAAGACGGCGG TGGAGTTCCTGTCCAAGGCTGTGGACAGTCCAAACATCAAGGCAGTGGACGAGGCTGTGATCTTGCTCCA GGAGATCGGGGTGCTGGACCAGCGGGAGTACCTGACTACCCTGGGGCAGCGCCTGGCTCACATCTCCACC GACCCCCGGTTGGCCAAGGCCATTGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTACTGGTGGTCG TTTCCTGCCTCACCCGGGACCCCTTCAGCAGCAGCCTACAGAACCGGGCAGAGGTGGACAAGGTGAAAGC ACTGTTGAGCCATGACAGCGGCAGTGACCACCTGGCCTTTGTGCGGGCTGTCGCCGGCTGGGAGGAGGTG CTGCGTTGGCAGGACCGCAGCTCCCGGGAGAATTACCTGGAGGAAAACCTGCTGTACGCACCCAGCCTGC GCTTCATCCACGGACTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCCTTCCTGGTGGGGAAGCCCTC GGACTGCACCCTGGCCTCCGCCCAGTGCAACGAGTACAGTGAGGAGGAGGAGCTGGTGAAGGGCGTGCTG ATGGCCGGCCTCTACCCCAACCTCATCCAGGTGAGGCAGGGCAAGGTCACCCGGCAGGGGAAGTTCAAGC CCAACAGCGTCACATATAGGACCAAATCAGGCAACATCCTGCTGCACAAGTCGACCATTAACAGGGAGGC CACACGGTTACGGAGCCGATGGCTGACGTATTTCATGGCAGTCAAGTCCAATGGCAGCGTCTTCGTCCGG GACTCCTCTCAGGTGCACCCGCTAGCTGTGCTGCTGCTGACCGACGGGGACGTGCACATCCGTGATGACG GGCGCCGGGCCACCATCTCACTGAGCGACAGTGACCTGCTGCGGCTGGAGGGTGACTCGCGTACCGTGCG GCTGCTGAAGGAGCTGCGGCGGGCCCTGGGCCGCATGGTGGAGCGGAGCCTGCGCAGCGAGCTGGCTGCA CTTCCCCCCAGCGTACAGGAGGAGCACGGGCAGCTGCTTGCGCTACTGGCAGAGCTGCTGCGAGGACCCT GTGGCAGCTTTGATGTGCGCAAGACAGCTGACGACTGA >OriGene 5' read for NM_014966 unedited
NGTTGGCGCATTTGTATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGGGCTCATG CGCGGTGCACAGAGGCTTGTTTCACATCTGTAACAACAGGAGGAGGCCCAGCCTCGTGAT GAGGAATAGCAAGGAGAGAATTCAGCTCCAGTTCAAAAGCCTACAAAATCTGAGACTGTC ATTGCTTTTATAAGGATTCCAGCTTTCCCTCCTGGCCAGAAATGTTCAGCCTGGACTCAT TCAGAAAAGATCGGGCCCAGCACAGGCAGCGTCAGTGCAAACTTCCCCCACCCCGCCTTC CACCCATGTGTGTCAACCCTACCCCAGGAGGGACCATCTCTCGAGCTTCTAGGGACCTAT TAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAAGAGCCCTCGGCA TCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAAAG TCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGA TCGATGCTGAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGC TAGGTCCCCGGAATGAGTTGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTG GCTCCCCTGCCGACAGCTGGTGGCGTCCGGAACCCACCATGCCCCCTACTTCCTGGCGGC AGCTGAATCCAGAGAGTATTCGACCAGGGNGGACCTGGGGGCCTATCCCGCTCTTTTAGG CCGGAGAAAAGAGAGACGAGGAGGAAGAGCTAGAAGAAAGGACCATAGATGTTACCGACT TCTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGNAGTTCCTT TGAGAGACAGATGACGACAGTGCCATTTAGGCTCTGACCCAGTT >OriGene 3' read for NM_014966 unedited
TGGCCGCGGCCGCAATCTATATCGAGTNCTTTTTTTTTTTTTTTTTTTTTTTCACACAAG GGATAAATAGAACTTTATTTTAAATAAACATTGGCACTCTGTACACAGCCCCAGCAAAAG CAGGGCTCATTCGTCAGCTGTCTTGCGCACATCAAAGCTGCCACAGGGTCCTCGCAGCAG CTCTGCCATTAGCGCAAGCAGCTGCCCGTGCTCCTCCTGTACGCTGGGGGGAAGTGCAGC CAGCTCGCTGCGCAGGCTCCGCTCCACCATGCGGCCCAGGGCCCGCCGAAGCTCCTTCAG CAGCCGCACGGTACGCGAGTCACCCTCCAGCCGCAGCAGGTCACTGTCGCTCATTGAGAT GGTGGCCCGGCGCCCGTCATCACGGATGTGCACGTCCCCGTCGGTCAGCAGCAGCACAGC TAGCGGGGGCCCCTGATAGGAGTCCCGGACAAAGACGCTGCCATTGGACTTGACGGCCAT GAAATACGCCAGCCCTCCGGCCCGGTATCCGTGGGGCCCCCCTGTAATGGGCCACTTGGG CACCAGGAAGCGGCCGGGTCTGGGTCCCTTTGTGACCCTGTTGGGCTTTATCTTCCCCCG CCCGGTTACTCGCTCCCCCTGCCTCGTTCCCGTTCGGGCTCACCCCCGCCCTCCCACCCC TCATACTCGCCCCCGGCGCCCCGTCCCCTAGCCTCCATAGCTTCCCTCTCCCCTCCCTTG CCCCCCCCCACCGCGACCCGCCCAGACCCGTGTGCTTTAGGTGCGCGCGTTTTCGCCCCT TCCCCCCACCACTACGCGCTCCATGCCCCACCCACTGTCTATCGAGCTTCCTTTTGTCTC CCCCCTTCCCTCCTCCAATCCCCTCGCGCCTTCCTGCGCCCGCTCGTTTCTCCTCCCGCC CCATTTTTTCTTTTTGCTTTCCTCCTTACACTTTCGTCGCTCGTCTCTCTTGCTGTCGCA TCGTCTTTTTTT |
Restriction Sites | NotI-NotI |
ACCN | NM_014966 |
Insert Size | 3670 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014966.2, NP_055781.2 |
RefSeq Size | 3880 bp |
RefSeq ORF | 3468 bp |
Locus ID | 22907 |
UniProt ID | Q7L2E3 |
Domains | DSRM, helicase_C, HA2 |
Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013] Transcript Variant: This variant (2) differs in its 5' UTR and uses an alternate translational start codon, compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, compared to isoform 1. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Human Mitochondrial DNA Nucleoids Are Linked to Protein Folding Machinery and Metabolic Enzymes at the Mitochondrial Inner Membrane
,Yousong Wang and Daniel F. Bogenhagen,
J. Biol. Chem., 2006 Sep 1;281(35):25791-802.
[DHX30]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206486 | DHX30 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2 |
CNY 8,040.00 |
|
RC206486L3 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2, Myc-DDK-tagged |
CNY 11,780.00 |
|
RC206486L4 | Lenti ORF clone of Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2, mGFP tagged |
CNY 11,780.00 |
|
RG206486 | DHX30 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2 |
CNY 9,640.00 |