DHX30 (NM_014966) Human Untagged Clone

CAT#: SC110281

DHX30 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2



  "NM_014966" in other vectors (4)

CNY 8,048.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-DHX30 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DDX30; NEDMIAL; RETCOR
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_014966, the custom clone sequence may differ by one or more nucleotides


ATGGCAGCTTCTAGGGACCTATTAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAA
GAGCCCTCGGCATCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAA
AGTCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGATCGATGCT
GAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGCTAGGTCCCCGGAATGAGT
TGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTGGCTCCCCTGCCGACAGCTGGTGGCGTCC
GGAACCCACCATGCCCCCTACTTCCTGGCGGCAGCTGAATCCAGAGAGTATTCGACCAGGGGGACCTGGG
GGCCTATCCCGCTCTTTAGGCCGGGAAGAAGAGGAGGACGAGGAGGAAGAGCTAGAAGAAGGGACCATAG
ATGTTACCGACTTCTTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGGAGTTC
CTTTGAGATGACAGATGACGACAGTGCCATTAGGGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTG
GCCAAGGTGATTCAGATTGCAACGTCATCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGCA
CCAAGACCAAGCTGTCTACACTCACCCTGCTCTGGCCCTGCCCCATGACCTTTGTTGCCAAAGGGCGCCG
CAAAGCAGAGGCTGAGAATAAGGCGGCAGCCTTGGCCTGCAAGAAACTGAAGAGCCTGGGCCTGGTGGAC
AGGAACAACGAACCGCTTACACACGCCATGTATAACCTGGCCTCTTTGCGTGAGCTGGGTGAGACCCAGC
GCCGACCATGCACCATCCAGGTGCCCGAGCCCATCCTCCGCAAGATAGAGACCTTCCTGAACCATTACCC
TGTGGAGAGTTCATGGATCGCCCCAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTGGGCAAGGAC
TCAGGGCCTCTGAGTGACCCTATCACAGGCAAGCCCTATGTGCCCCTGTTGGAAGCAGAGGAGGTACGTC
TCAGCCAGAGTCTGCTAGAACTGTGGCGGCGGCGAGGGCCGGTCTGGCAGGAGGCCCCCCAGCTACCTGT
GGACCCACATCGGGACACCATCCTCAACGCCATTGAGCAGCACCCGGTGGTGGTCATCTCTGGGGACACG
GGCTGTGGGAAGACCACGCGCATCCCCCAGCTGTTGCTGGAGCGCTATGTGACCGAGGGCCGAGGTGCCC
GCTGCAATGTTATCATCACCCAACCTCGCCGCATCTCTGCTGTGTCTGTGGCACAGCGGGTCAGCCACGA
ACTGGGCCCCTCCCTGCGCCGGAATGTGGGCTTCCAGGTGCGGTTGGAAAGTAAGCCCCCATCCCGAGGC
GGGGCCCTGCTCTTCTGCACTGTGGGTATCCTGCTGCGTAAGCTGCAGAGCAACCCCAGCCTGGAGGGCG
TGAGCCACGTCATCGTGGATGAGGTGCATGAGCGGGACGTGAACACAGACTTTCTGCTGATCCTGCTCAA
GGGCCTGCAGCGGCTCAACCCGGCCCTGCGGCTGGTGCTCATGAGTGCCACAGGGGACAATGAGCGCTTC
TCCCGATACTTTGGTGGCTGCCCCGTCATCAAGGTGCCTGGCTTCATGTACCCAGTCAAGGAGCACTACC
TAGAGGACATCCTGGCCAAGTTGGGCAAGCACCAGTACCTGCACCGGCACCGGCACCATGAGTCTGAGGA
TGAATGCGCACTCGATTTGGACCTTGTGACTGATCTGGTTCTGCACATCGATGCTCGCGGGGAACCAGGT
GGGATCCTGTGCTTCCTGCCTGGGTGGCAGGAGATCAAAGGAGTGCAGCAGCGCCTCCAGGAGGCCCTGG
GCATGCACGAGAGCAAGTACCTCATCCTGCCAGTGCACTCCAACATCCCCATGATGGATCAGAAGGCCAT
ATTCCAGCAGCCTCCAGTTGGGGTGCGCAAGATTGTCTTGGCCACCAACATTGCTGAGACTTCCATCACA
ATCAATGACATCGTGCATGTGGTGGACAGTGGGCTGCACAAGGAAGAACGCTATGACCTGAAGACCAAGG
TGTCCTGCCTGGAGACAGTGTGGGTATCAAGAGCCAATGTGATCCAGCGCCGGGGCCGGGCGGGCCGCTG
CCAGTCCGGCTTTGCCTACCACTTGTTCCCTCGAAGCCGGCTGGAGAAAATGGTCCCTTTCCAAGTGCCA
GAGATCCTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCGAAAATCCACATGCCTGAGAAGACGGCGG
TGGAGTTCCTGTCCAAGGCTGTGGACAGTCCAAACATCAAGGCAGTGGACGAGGCTGTGATCTTGCTCCA
GGAGATCGGGGTGCTGGACCAGCGGGAGTACCTGACTACCCTGGGGCAGCGCCTGGCTCACATCTCCACC
GACCCCCGGTTGGCCAAGGCCATTGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTACTGGTGGTCG
TTTCCTGCCTCACCCGGGACCCCTTCAGCAGCAGCCTACAGAACCGGGCAGAGGTGGACAAGGTGAAAGC
ACTGTTGAGCCATGACAGCGGCAGTGACCACCTGGCCTTTGTGCGGGCTGTCGCCGGCTGGGAGGAGGTG
CTGCGTTGGCAGGACCGCAGCTCCCGGGAGAATTACCTGGAGGAAAACCTGCTGTACGCACCCAGCCTGC
GCTTCATCCACGGACTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCCTTCCTGGTGGGGAAGCCCTC
GGACTGCACCCTGGCCTCCGCCCAGTGCAACGAGTACAGTGAGGAGGAGGAGCTGGTGAAGGGCGTGCTG
ATGGCCGGCCTCTACCCCAACCTCATCCAGGTGAGGCAGGGCAAGGTCACCCGGCAGGGGAAGTTCAAGC
CCAACAGCGTCACATATAGGACCAAATCAGGCAACATCCTGCTGCACAAGTCGACCATTAACAGGGAGGC
CACACGGTTACGGAGCCGATGGCTGACGTATTTCATGGCAGTCAAGTCCAATGGCAGCGTCTTCGTCCGG
GACTCCTCTCAGGTGCACCCGCTAGCTGTGCTGCTGCTGACCGACGGGGACGTGCACATCCGTGATGACG
GGCGCCGGGCCACCATCTCACTGAGCGACAGTGACCTGCTGCGGCTGGAGGGTGACTCGCGTACCGTGCG
GCTGCTGAAGGAGCTGCGGCGGGCCCTGGGCCGCATGGTGGAGCGGAGCCTGCGCAGCGAGCTGGCTGCA
CTTCCCCCCAGCGTACAGGAGGAGCACGGGCAGCTGCTTGCGCTACTGGCAGAGCTGCTGCGAGGACCCT
GTGGCAGCTTTGATGTGCGCAAGACAGCTGACGACTGA


>OriGene 5' read for NM_014966 unedited
NGTTGGCGCATTTGTATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGGGCTCATG
CGCGGTGCACAGAGGCTTGTTTCACATCTGTAACAACAGGAGGAGGCCCAGCCTCGTGAT
GAGGAATAGCAAGGAGAGAATTCAGCTCCAGTTCAAAAGCCTACAAAATCTGAGACTGTC
ATTGCTTTTATAAGGATTCCAGCTTTCCCTCCTGGCCAGAAATGTTCAGCCTGGACTCAT
TCAGAAAAGATCGGGCCCAGCACAGGCAGCGTCAGTGCAAACTTCCCCCACCCCGCCTTC
CACCCATGTGTGTCAACCCTACCCCAGGAGGGACCATCTCTCGAGCTTCTAGGGACCTAT
TAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAAGAGCCCTCGGCA
TCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAAAG
TCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGA
TCGATGCTGAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGC
TAGGTCCCCGGAATGAGTTGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTG
GCTCCCCTGCCGACAGCTGGTGGCGTCCGGAACCCACCATGCCCCCTACTTCCTGGCGGC
AGCTGAATCCAGAGAGTATTCGACCAGGGNGGACCTGGGGGCCTATCCCGCTCTTTTAGG
CCGGAGAAAAGAGAGACGAGGAGGAAGAGCTAGAAGAAAGGACCATAGATGTTACCGACT
TCTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGNAGTTCCTT
TGAGAGACAGATGACGACAGTGCCATTTAGGCTCTGACCCAGTT
>OriGene 3' read for NM_014966 unedited
TGGCCGCGGCCGCAATCTATATCGAGTNCTTTTTTTTTTTTTTTTTTTTTTTCACACAAG
GGATAAATAGAACTTTATTTTAAATAAACATTGGCACTCTGTACACAGCCCCAGCAAAAG
CAGGGCTCATTCGTCAGCTGTCTTGCGCACATCAAAGCTGCCACAGGGTCCTCGCAGCAG
CTCTGCCATTAGCGCAAGCAGCTGCCCGTGCTCCTCCTGTACGCTGGGGGGAAGTGCAGC
CAGCTCGCTGCGCAGGCTCCGCTCCACCATGCGGCCCAGGGCCCGCCGAAGCTCCTTCAG
CAGCCGCACGGTACGCGAGTCACCCTCCAGCCGCAGCAGGTCACTGTCGCTCATTGAGAT
GGTGGCCCGGCGCCCGTCATCACGGATGTGCACGTCCCCGTCGGTCAGCAGCAGCACAGC
TAGCGGGGGCCCCTGATAGGAGTCCCGGACAAAGACGCTGCCATTGGACTTGACGGCCAT
GAAATACGCCAGCCCTCCGGCCCGGTATCCGTGGGGCCCCCCTGTAATGGGCCACTTGGG
CACCAGGAAGCGGCCGGGTCTGGGTCCCTTTGTGACCCTGTTGGGCTTTATCTTCCCCCG
CCCGGTTACTCGCTCCCCCTGCCTCGTTCCCGTTCGGGCTCACCCCCGCCCTCCCACCCC
TCATACTCGCCCCCGGCGCCCCGTCCCCTAGCCTCCATAGCTTCCCTCTCCCCTCCCTTG
CCCCCCCCCACCGCGACCCGCCCAGACCCGTGTGCTTTAGGTGCGCGCGTTTTCGCCCCT
TCCCCCCACCACTACGCGCTCCATGCCCCACCCACTGTCTATCGAGCTTCCTTTTGTCTC
CCCCCTTCCCTCCTCCAATCCCCTCGCGCCTTCCTGCGCCCGCTCGTTTCTCCTCCCGCC
CCATTTTTTCTTTTTGCTTTCCTCCTTACACTTTCGTCGCTCGTCTCTCTTGCTGTCGCA
TCGTCTTTTTTT
Restriction Sites NotI-NotI     
ACCN NM_014966
Insert Size 3670 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014966.2, NP_055781.2
RefSeq Size 3880 bp
RefSeq ORF 3468 bp
Locus ID 22907
UniProt ID Q7L2E3
Domains DSRM, helicase_C, HA2
Gene Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]
Transcript Variant: This variant (2) differs in its 5' UTR and uses an alternate translational start codon, compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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