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TCIRG1 (NM_006053) Human Untagged Clone
CAT＃: SC110144
TCIRG1 (untagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 2
CNY 4,576.00
CNY 7,220.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC110144 representing NM_006053.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GAATTCGGCACGAGGGACGGAGCGCCTGGCCCAGGAGCTGCGGGATGTGCGGGGCAACCAGCAGGCCCT GCGGGCCCAGCTGCACCAGCTGCAGCTCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTGGC AGCCGCCCACACAGATGGGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCAGGA CCTGAGGGTCAACTTTGTGGCAGGTGCCGTGGAGCCCCACAAGGCCCCTGCCCTAGAGCGCCTGCTCTG GAGGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCACCCCGTGAC GGGCGAGCCAGCCACGTGGATGACCTTCCTCATCTCCTACTGGGGTGAGCAGATCGGACAGAAGATCCG CAAGATCACGGACTGCTTCCACTGCCACGTCTTCCCGTTTCTGCAGCAGGAGGAGGCCCGCCTCGGGGC CCTGCAGCAGCTGCAACAGCAGAGCCAGGAGCTGCAGGAGGTCCTCGGGGAGACAGAGCGGTTCCTGAG CCAGGTGCTAGGCCGGGTGCTGCAGCTGCTGCCGCCAGGGCAGGTGCAGGTCCACAAGATGAAGGCCGT GTACCTGGCCCTGAACCAGTGCAGCGTGAGCACCACGCACAAGTGCCTCATTGCCGAGGCCTGGTGCTC TGTGCGAGACCTGCCCGCCCTGCAGGAGGCCCTGCGGGACAGCTCGATGGAGGAGGGAGTGAGTGCCGT GGCTCACCGCATCCCCTGCCGGGACATGCCCCCCACACTCATCCGCACCAACCGCTTCACGGCCAGCTT CCAGGGCATCGTGGATGCCTACGGCGTGGGCCGCTACCAGGAGGTCAACCCCGCTCCCTACACCATCAT CACCTTCCCCTTCCTGTTTGCTGTGATGTTCGGGGATGTGGGCCACGGGCTGCTCATGTTCCTCTTCGC CCTGGCCATGGTCCTTGCGGAGAACCGACCGGCTGTGAAGGCCGCGCAGAACGAGATCTGGCAGACTTT CTTCAGGGGCCGCTACCTGCTCCTGCTTATGGGCCTGTTCTCCATCTACACCGGCTTCATCTACAACGA GTGCTTCAGTCGCGCCACCAGCATCTTCCCCTCGGGCTGGAGTGTGGCCGCCATGGCCAACCAGTCTGG CTGGAGTGATGCATTCCTGGCCCAGCACACGATGCTTACCCTGGATCCCAACGTCACCGGTGTCTTCCT GGGACCCTACCCCTTTGGCATCGATCCTATTTGGAGCCTGGCTGCCAACCACTTGAGCTTCCTCAACTC CTTCAAGATGAAGATGTCCGTCATCCTGGGCGTCGTGCACATGGCCTTTGGGGTGGTCCTCGGAGTCTT CAACCACGTGCACTTTGGCCAGAGGCACCGGCTGCTGCTGGAGACGCTGCCGGAGCTCACCTTCCTGCT GGGACTCTTCGGTTACCTCGTGTTCCTAGTCATCTACAAGTGGCTGTGTGTCTGGGCTGCCAGGGCCGC CTCGGCCCCCAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCTCCCACAGCCCCAGCAACAGGCT GCTCTACCCCCGGCAGGAGGTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCCATGGTGCCCATCCT GCTGCTTGGCACACCCCTGCACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCCGCTGACCG ACAGGAGGAAAACAAGGCCGGGTTGCTGGACCTGCCTGACGCATCTGTGAATGGCTGGAGCTCCGATGA GGAAAAGGCAGGGGGCCTGGATGATGAAGAGGAGGCCGAGCTCGTCCCCTCCGAGGTGCTCATGCACCA GGCCATCCACACCATCGAGTTCTGCCTGGGCTGCGTCTCCAACACCGCCTCCTACCTGCGCCTGTGGGC CCTGAGCCTGGCCCACGCCCAGCTGTCCGAGGTTCTGTGGGCCATGGTGATGCGCATAGGCCTGGGCCT GGGCCGGGAGGTGGGCGTGGCGGCTGTGGTGCTGGTCCCCATCTTTGCCGCCTTTGCCGTGATGACCGT GGCTATCCTGCTGGTGATGGAGGGACTCTCAGCCTTCCTGCACGCCCTGCGGCTGCACTGGGTGGAATT CCAGAACAAGTTCTACTCAGGCACGGGCTACAAGCTGAGTCCCTTCACCTTCGCTGCCACAGATGACTA GGGCCCACTGCAGGTCCTGCCAGACCTCCTTCCTGACCTCTGAGGCAGGAGAGGAATAAAGACGGTCCG CCCTGGCAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_006053 unedited
GTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATCGGCACGAGGGACGGAGCGC CTGGCCCAGNAGCTGCGGGATGTGCGGGGCAACCAGCAGGCCCTGCGGGCCCAGCTGCAC CAGCTGCAGCTCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTGGCAGCCGCC CACACAGATGGGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCAG GACCTGAGGGTCAACTTTGTGGCAGGTGCCGTGGAGCCCCACAAGGCCCCTGCCCTAGAG CGCCTGCTCTGGAGGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAG CCGCTGGAGCACCCCGTGACGGGCGAGCCAGCCACGTGGATGACCTTCCTCATCTCCTAC TGGGGTGAGCAGATCGGACAGAAGATCCGCAAGATCACGGACTGCTTCCACTGCCACGTC TTCCCGTTTCTGCAGCAGGAGGAGGCCCGCCTCGGGGCCCTGCAGCAGCTGCAACAGCAG AGCCAGGAGCTGCAGGAGGTCCTCGGGGAGACAGAGCGGTTCCTGAGCCAGGTGCTAGGC CGGGTGCTGCAGCTGCTGCCGCCAGGGCAGGTGCAGGTCCACAAGATGAAGGCCGTGTAC CTGGCCCTGAACCAGTGCAGCGTGAGCACCACGCACAAGTGCCTCATTGCCGAGGCCTGG TGCTCTGTGCGAGACCTGCCCGCCCTGCAGGAGGCCCTGCGGGACAGCTCGATGGAGGAG GGAGTGAGTGCCGTGGCTCACCGCATCCCCTGCCGGGACATGCCCNCCACACTCATCCGC ACCAACCGCTTCACGGNCAGCTTCCCAGGCATCGTGGATGCCTACGGCGTGGGCCGCTAC CAGAAGGTCAACCCCGCTTCCTACACCAT >OriGene 3' read for NM_006053 unedited
TGGGACGGACCGCCTTTTTTCCTCTCCTGCCTCANAGTCAGGNAAGAGGTCTGGCAGGAC CTGCAGTGGGCCCTAGTCATCTGTGGCAGCGAAGGTGAAGGGACTCAGCTTGTAGCCCGT GCCTGAGTAGAACTTGTTCTGGAATTCCACCCAGTGCAGCCGCAGGGCGTGCAGGAAGGC TGAGAGTCCCTCCATCACCAGCAGGATAGCCACGGTCATCACGGCAAAGGCGGCAAAGAT GGGGACCAGCACCACAGCCGCCACGCCCACCTCCCGGCCCAGGCCCAGGCCTATGCGCAT CACCATGGCCCACAGAACCTCGGACAGCTGGGCGTGGGCCAGGCTCAGGGCCCACAGGCG CAGGTAGGAGGCGGTGTTGGAGACGCAGCCCAGGCAGAACTCGATGGTGTGGATGGCCTG GTGCATGAGCACCTTGGAGGGGACGAGCTCGGCCTCCTCTTCATCATCCAGGCCCCCTGC CTTTTCCTCATCGGAGCTNCAGCCATTCACAGATGCGTCAGGCAGGTCCAGCAACCCGGC CTTGTTTTCCTCCTGTCGGTCAGCGGGCCTCCTCCGCATGCGGNGGCGGTGGCGGTGCAC CATGTGCACGGGTGTGCCAAGCAGCATGATGGGCACCATGGCCAAGGCCAGAACCACCAG CGTGGCCTGGGACACCTCCTGCCCGGGGTTGAGNANCCTGTTGCTGGGGCTGCGGGAGAA GAGGACATGTCTGTGAATTGGATGGAGATGCTTGGGGCCGAGGCGGCCCTGNNACCCACC ACCCCCTGCACCTTGGTTAGACTAGAACACAGCAGTAACCTAAGATTCCCACAAGGAAGG CATGCTTCTGTATGTTTCCTATCACTCCCCGTGCCTTTGGGTCAATGGAACTTGGTTNAN ACCTCCTGGTCACCCCTAAGCGCTGTCAACTATCCACTNCTACCGCTCCTCCTCTTTACG GTTTGTACACGCCTGTCCCACTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006053 |
| Insert Size | 2323 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006053.2 |
| RefSeq Size | 2480 bp |
| RefSeq ORF | 2323 bp |
| Locus ID | 10312 |
| UniProt ID | Q13488 |
| Domains | V_ATPase_sub_a |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| MW | 79.9 kDa |
| Gene Summary | This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017] Transcript Variant: This variant (2) encodes isoform b, also known as the TCIR7 protein. This protein is expressed in T lymphocytes and is essential for normal T cell activation. This variant uses a transcription start site that is within exon 5 of variant 1, and it also includes the following intron as part of its 5' UTR. The encoded isoform (b) is shorter than isoform a. |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207157 | TCIRG1 (Myc-DDK-tagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 2 |
CNY 6,088.00 |
|
| RC207157L3 | Lenti ORF clone of Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207157L4 | Lenti ORF clone of Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG207157 | TCIRG1 (tGFP-tagged) - Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 2 |
CNY 5,040.00 |
