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AKAP12 (NM_005100) Human Untagged Clone
CAT＃: SC110078
AKAP12 (untagged)-Human A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 1
CNY 11,984.00
货期*
现货
规格
| Cited in 2 publications. |
Product images
经常一起买 (4)
AKAP12 mouse monoclonal antibody,clone OTI1B6
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AKAP250; SSeCKS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_005100 edited
TTCGGCACGAGGGTTTGCCGCGAGCGCGTCTCCTTCATTCGCAGGCTGGGCGCGTTCGCA GTCGGCTGGCGGCGAAGGAAGGCGCTCTCGGGACCTCGCGGGCGCGCGTCTTTTGGCTCT TGCCCCTGTCCCTGCGGCTTGGGGAAGGCGTAACCCGGCGGCTAGGCGCGGGAGAAGTGC GGAGGAGCCATGGGCGCCGGGAGCTCCACCGAGCAGCGCAGCCCGGAGCAGCCGCCCGAG GGGAGCTCCACGCCGGCTGAGCCCGAGCCCAGCGGCGGCGGCCCCTCGGCCGAGGCGGCG CCAGACACCACCGCGGACCCCGCCATCGCTGCCTCGGACCCCGCCACCAAGCTCCTACAG AAGAATGGTCAGCTGTCCACCATCAATGGCGTAGCTGAGCAAGATGAGCTCAGCCTCCAG GAGGGTGACCTAAATGGCCAGAAAGGAGCCCTGAACGGTCAAGGAGCCCTAAACAGCCAG GAGGAAGAAGAAGTCATTGTCACAGAGGTTGGACAGAGAGACTCTGAAGATGTGAGCAAA AGAGACTCCGATAAAGAGATGGCTACTAAGTCAGCGGTTGTTCACGACATCACAGATGAT GGGCAGGAGGAGACACCCGAAATAATCGAACAGATTCCTTCTTCAGAAAGCAATTTAGAA GAGCTAACACAACCCACTGAGTCCCAGGCTAATGATATTGGATTTAAGAAGGTGTTTAAG TTTGTTGGCTTTAAATTCACTGTGAAAAAGGATAAGACAGAGAAGCCTGACACTGTCCAG CTACTCACTGTGAAGAAAGATGAAGGGGAGGGAGCAGCAGGGGCTGGCGACCACAAGGAC CCCAGCCTTGGGGCTGGAGAAGCAGCATCCAAAGAAAGCGAACCCAAACAATCTACAGAG AAACCCGAAGAGACCCTGAAGCGTGAGCAAAGCCACGCAGAAATTTCTCCCCCAGCCGAA TCTGGCCAAGCAGTGGAGGAATGCAAAGAGGAAGGAGAAGAGAAACAAGAAAAAGAACCT AGCAAGTCTGCAGAATCTCCGACTAGTCCCGTGACCAGTGAAACAGGATCAACCTTCAAA AAATTCTTCACTCAAGGTTGGGCCGGCTGGCGCAAAAAGACCAGTTTCAGGAAGCCGAAG GAGGATGAAGTGGAAGCTTCAGAGAAGAAAAAGGAACAAGAGCCAGAAAAAGTAGACACA GAAGAAGACGGAAAGGCAGAGGTTGCCTCCGAGAAACTGACCGCCTCCGAGCAAGCCCAC CCACAGGAGCCGGCAGAAAGTGCCCACGAGCCCCGGTTATCAGCTGAATATGAGAAAGTT GAGCTGCCCTCAGAGGAGCAAGTCAGTGGCTCGCAGGGACCTTCTGAAGAGAAACCTGCT CCGTTGGCGACAGAAGTGTTTGATGAGAAAATAGAAGTCCACCAAGAAGAGGTTGTGGCC GAAGTCCACGTCAGCACCGTGGAGGAGAGAACCGAAGAGCAGAAAACGGAGGTGGAAGAA ACAGCAGGGTCTGTGCCAGCTGAAGAATTGGTTGAAATGGATGCAGAACCTCAGGAAGCT GAACCTGCCAAGGAGCTGGTGAAGCTCAAAGAAACGTGTGTTTCCGGAGAGGACCCTACA CAGGGAGCTGACCTCAGTCCTGATGAGAAGGTGCTGTCCAAACCCCCCGAAGGCGTTGTG AGTGAGGTGGAAATGCTGTCATCACAGGAGAGAATGAAGGTGCAGGGAAGTCCACTAAAG AAGCTTTTTACCAGCACTGGCTTAAAAAAGCTTTCTGGAAAGAAACAGAAAGGGAAAAGA GGAGGAGGAGACGAGGAATCAGGGGAGCACACTCAGGTTCCAGCCGATTCTCCGGACAGC CAGGAGGAGCAAAAGGGCGAGAGCTCTGCCTCATCCCCTGAGGAGCCCGAGGAGATCACG TGTCTGGAAAAGGGCTTAGCCGAGGTGCAGCAGGATGGGGAAGCTGAAGAAGGAGCTACT TCCGATGGAGAGAAAAAAAGAGAAGGTGTCACTCCCTGGGCATCATTCAAAAAGATGGTG ACGCCCAAGAAGCGTGTTAGACGGCCTTCGGAAAGTGATAAAGAAGATGAGCTGGACAAG GTCAAGAGCGCTACCTTGTCTTCCACCGAGAGCACAGCCTCTGAAATGCAAGAAGAAATG AAAGGGAGCGTGGAAGAGCCAAAGCCGGAAGAACCAAAGCGCAAGGTGGATACCTCAGTA TCTTGGGAAGCTTTAATTTGTGTGGGATCATCCAAGAAAAGAGCAAGGAGAGGGTCCTCT TCTGATGAGGAAGGGGGACCAAAAGCAATGGGAGGAGACCACCAGAAAGCTGATGAGGCC GGAAAAGACAAAGAGACGGGGACAGACGGGATCCTTGCTGGTTCCCAAGAACATGATCCA GGGCAGGGAAGTTCCTCCCCGGAGCAAGCTGGAAGCCCTACCGAAGGGGAGGGCGTTTCC ACCTGGGAGTCATTTAAAAGGTTAGTCACGCCAAGAAAAAAATCAAAGTCCAAGCTGGAA GAGAAAAGCGAAGACTCCATAGCTGGGTCTGGTGTAGAACATTCCACTCCAGACACTGAA CCCGGTAAAGAAGAATCCTGGGTCTCAATCAAGAAGTTTATTCCTGGACGAAGGAAGAAA AGGCCAGATGGGAAACAAGAACAAGCCCCTGTTGAAGACGCAGGGCCAACAGGGGCCAAC GAAGATGACTCTGATGTCCCGGCCGTGGTCCCTCTGTCTGAGTATGATGCTGTAGAAAGG GAGAAAATGGAGGCACAGCAAGCCCAAAAAAGCGCAGAGCAGCCCGAGCAGAAGGCAGCC ACTGAGGTGTCCAAGGAGCTCAGCGAGAGTCAGGTTCATATGATGGCAGCAGCTGTCGCT GACGGGACGAGGGCAGCTACCATTATTGAAGAAAGGTCTCCTTCTTGGATATCTGCTTCA GTGACAGAACCTCTTGAACAAGTAGAAGCTGAAGCCGCACTGTTAACTGAGGAGGTATTG GAAAGAGAAGTAATTGCAGAAGAAGAACCCCCCACGGTTACTGAACCTCTGCCAGAGAAC AGAGAGGCCCGGGGCGACACGGTCGTTAGTGAGGCGGAATTGACCCCCGAAGCTGTGACA GCTGCAGAAACTGCAGGGCCATTGGGTGCCGAAGAAGGAACCGAAGCATCTGCTGCTGAA GAGACCACAGAAATGGTGTCAGCAGTCTCCCAGTTAACCGACTCCCCAGACACCACAGAG GAGGCCACTCCGGTGCAGGAGGTGGAAGGTGGCGTACCTGACATAGAAGAGCAAGAGAGG CGGACTCAAGAGGTCCTCCAGGCAGTGGCAGAAAAAGTGAAAGAGGAATCCCAGCTGCCT GGCACCGGTGGGCCAGAAGATGTGCTTCAGCCTGTGCAGAGAGCAGAGGCAGAAAGACCA GAAGAGCAGGCTGAAGCGTCGGGTCTGAAGAAAGAGACGGATGTAGTGTTGAAAGTAGAT GCTCAGGAGGCAAAAACTGAGCCTTTTACACAAGGGAAGGTGGTGGGGCAGACCACCCCA GAAAGCTTTGAAAAAGCTCCTCAAGTCACAGAGAGCATAGAGTCCAGTGAGCTTGTAACC ACTTGTCAAGCCGAAACCTTAGCTGGGGTAAAATCACAGGAGATGGTGATGGAACAGGCT ATCCCCCCTGACTCGGTGGAAACCCCTACAGACAGTGAGACTGATGGAAGCACCCCCGTA GCCGACTTTGACGCACCAGGCACAACCCAGAAAGACGAGATTGTGGAAATCCATGAGGAG AATGAGGTCGCATCTGGTACCCAGTCAGGGGGCACAGAAGCAGAGGCAGTTCCTGCACAG AAAGAGAGGCCTCCAGCACCTTCCAGTTTTGTGTTCCAGGAAGAAACTAAAGAACAATCA AAGATGGAAGACACTCTAGAGCATACAGATAAAGAGGTGTCAGTGGAAACTGTATCCATT CTGTCAAAGACTGAGGGGACTCAAGAGGCTGACCAGTATGCTGATGAGAAAACCAAAGAC GTACCATTTTTCGAAGGACTTGAGGGGTCTATAGACACAGGCATAACAGTCAGTCGGGAA AAGGTCACTGAAGTTGCCCTTAAAGGTGAAGGGACAGAAGAAGCTGAATGTAAAAAGGAT GATGCTCTTGAACTGCAGAGTCACGCTAAGTCTCCTCCATCCCCCGTGGAGAGAGAGATG GTAGTTCAAGTCGAAAGGGAGAAAACAGAAGCAGAGCCAACCCATGTGAATGAAGAGAAG CTTGAGCACGAAACAGCTGTTACCGTATCTGAAGAGGTCAGTAAGCAGCTCCTCCAGACA GTGAATGTGCCCATCATAGATGGGGCAAAGGAAGTCAGCAGTTTGGAAGGAAGCCCTCCT CCCTGCCTAGGTCAAGAGGAGGCAGTATGCACCAAAATTCAAGTTCAGAGCTCTGAGGCA TCATTCACTCTAACAGCGGCTGCAGAGGAGGAAAAGGTCTTAGGAGAAACTGCCAACATT TTAGAAACAGGTGAAACGTTGGAGCCTGCAGGTGCACATTTAGTTCTGGAAGAGAAATCC TCTGAAAAAAATGAAGACTTTGCCGCTCATCCAGGGGAAGATGCTGTGCCCACAGGGCCC GACTGTCAGGCAAAATCGACACCAGTGATAGTATCTGCTACTACCAAGAAAGGCTTAAGT TCGGACCTGGAAGGAGAGAAAACCACATCACTGAAGTGGAAGTCAGATGAAGTCGATGAG CAGGTTGCTTGCCAGGAGGTCAAAGTGAGTGTAGCAATTGAGGATTTAGAGCCTGAAAAT GGGATTTTGGAACTTGAGACCAAAAGCAGTAAACTTGTCCAAAACATCATCCAGACAGCC GTTGACCAGTTTGTACGTACAGAAGAAACAGCCACCGAAATGTTGACGTCTGAGTTACAG ACACAAGCTCACGTGATAAAAGCTGACAGCCAGGACGCTGGACAGGAAACGGAGAAAGAA GGAGAGGAACCTCAGGCCTCTGCACAGGATGAAACACCAATTACTTCAGCCAAAGAGGAG TCAGAGTCAACCGCAGTGGGACAAGCACATTCTGATATTTCCAAAGACATGAGTGAAGCC TCAGAAAAGACCATGACTGTTGAGGTAGAAGGTTCCACTGTAAATGATCAGCAGCTGGAA GAGGTCGTCCTCCCATCTGAGGAAGAGGGAGGTGGAGCTGGAACAAAGTCTGTGCCAGAA GATGATGGTCATGCCTTGTTAGCAGAAAGAATAGAGAAGTCACTAGTTGAACCGAAAGAA GATGAAAAAGGTGATGATGTTGATGACCCTGAAAACCAGAACTCAGCCCTGGCTGATACT GATGCCTCAGGAGGCTTAACCAAAGAGTCCCCAGATACAAATGGACCAAAACAAAAAGAG AAGGAGGATGCCCAGGAAGTAGAATTGCAGGAAGGAAAAGTGCACAGTGAATCAGATAAA GCGATCACACCCCAAGCACAGGAGGAGTTACAGAAACAAGAGAGAGAATCTGCAAAGTCA GAACTTACAGAATCTTAAAACATCATGCAGTTAAACTCATTGTCTGTTTGGAAGACCAGA ATGTGAAGACAAGTAGTAGAAGAAAATGAATGCTGCTGCTGAGACTGAAGACCAGTATTT CAGAACTTTGAGAATTGGAGAGCAGGCACATCAACTGATCTCATTTCTAGAG >OriGene 5' read for NM_005100 unedited
TGAATTAGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGTTTGCCGCGAGCG CGTCTCCTTCATTCGCAGGCTGGGCGCGTTCGCAGTCGGCTGGCGGCGAAGGAAGGCGCT CTCGGGACCTCGCGGGCGCGCGTCTTTTGGCTCTTGCCCCTGTCCCTGCGGCTTGGGGAA GGCGTAACCCGGCGGCTAGGCGCGGGAGAAGTGCGGAGGAGCCATGGGCGCCGGGAGCTC CACCGAGCAGCGCAGCCCGGAGCAGCCGCCCGAGGGGAGCTCCACGCCGGCTGAGCCCGA GCCCAGCGGCGGCGGCCCCTCGGCCGAGGCGGCGCCAGACACCACCGCGGACCCCGCCAT CGCTGCCTCGGACCCCGCCACCAAGCTCCTACAGAAGAATGGTCAGCTGTCCACCATCAA TGGCGTAGCTGAGCAAGATGAGCTCAGCCTCCAGGAGGGTGACCTAAATGGCCAGAAAGG AGCCCTGAACGGTCAAGGAGCCCTAAACAGCCAGGAGGAAGAAGAAGTCATTGTCACAGA GGTTGGACAGAGAGACTCTGAAGATGTGAGCAAAAGAGACTCCGATAAAGAGATGGCTAC TAAGTCAGCGGTTGTTCACGACATCACAGATGATGGGCAGGAGGAGACACCCGAAATAAT CGAACAGATTCCTTCTTCAGANAGCAATTTAGAAGAGCTAACACAACCCACTGAGTCCCA GGCTAATGATATTGGATTTAAGAAGGTGGTTAAGTTTGTTGGCTTTAAAATCACTGTNGA AAAGGATNAGACAGAGAAGCCTGACACTGTCCAGCTACTCACTGTGAAGAAAGATGAAGG GGAGGGAGCAGCAGGNGCTGGCGACCACAAGGACCCAGCCTTGNNGCTGNAGAAGCAGCA TCCACAGAAGCGAACCCAACATCTACGAGAAACCCGAGAGACCTG >OriGene 3' read for NM_005100 unedited
TGTAACGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTAAGTGAAGCAAA TATTTTATTTAAATCAGTCGTCAAATCACAACTTATCCAAATGAATGTGTTGCAACATTG TTCTTAAAAGAATGGCACAAATATGGCACAAACACAATCCAGTATCTATCAAAATACCAT TTGTAAAGAACCTGACTTATTTCATGCCATGTGTGAATGCAGTCCAGCATATAAGAGTAG AATCAAAAACTTACAACAATTCTTTTCTTCATAACATATAAAACATATTCACTACTTTGC CTCAGGATTAAGCACACTTATCGGTCCAAACAACCATAACAAGTAGGAAAAAGGATGTAA AGTTAATTTGCTACCAGAATATAACTTCTTTCTAACCTCCACTGGGTTAGAAAGAAAAAT TTCCCAATGACAATAGAACATTCTGTATGTTTCAAAATGCCTTTTTAGTTTTAGCACACT CCGTGTAATAGGAACGTGACCAATTTCAGAGCACCACTAAATATGGACCTCAATCATTAG CGAATTTTTAAAGAATTGCACCTTGATCAGGAATGTTACATCTAGGAGGCTCTGTTTTTC CGGGCTATATAACTGAAGCTTAAAGCCTCCCAAGCCTGCACTATGGCACATGCCCCATCA TATACTGGGGGATACAAAAACCTGGAGTATCCTGCCCATTCCCTTATACTTTAGAATAAA TTTCCACTTGATACCGGAGGACTACTTAACCACTTCATATAAATTTGGAAAGAATGGATA CTCAATTTCCACAACCAAAACTAGGTTTTGCCAACTTCAGTTCCAGGTTAAGAATTTAAG GATCTCTAATCACAATCTATTAGGCGTTCAAGCGAAATCGGCCGTTGGTCCTTGCAACAG AAAAGGCAAAGGCCCTAACCTCCGATAAACCCTAGGAATGCCAGAGGCCTTCTAAAAGTA ATACATGAGGGCCCGGCTCCACATTCCAAANTTTGAAAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005100 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005100.2, NP_005091.2 |
| RefSeq Size | 6609 bp |
| RefSeq ORF | 5349 bp |
| Locus ID | 9590 |
| UniProt ID | Q02952 |
| Domains | PkinA_anch |
| Protein Families | Druggable Genome |
| Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Re-Expression of AKAP12 Inhibits Progression and Metastasis Potential of Colorectal Carcinoma In Vivo and In Vitro
,null,
PLoS ONE
,PubMed ID 21918680
[AKAP12]
|
|
Quantitative assessment of AKAP12 promoter methylation in colorectal cancer using methylation-sensitive high resolution melting: Correlation with Duke's stage.
,null,
Cancer biology & therapy
,PubMed ID 20364105
[AKAP12]
|
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