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Vinculin (VCL) (NM_014000) Human Untagged Clone
CAT＃: SC109880
VCL (untagged)-Human vinculin (VCL), transcript variant 1
CNY 21,850.00
货期*
7周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CMD1W; CMH15; HEL114; MV; MVCL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_014000, the custom clone sequence may differ by one or more nucleotides
ATGCCAGTGTTTCATACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCACAGCAGATCTCCCACCTGG TGATAATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTGGCCGCCGT GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAGACTGTTCAAACCACTGAGGATCAGATTTTG AAGAGAGATATGCCACCAGCATTTATTAAGGTTGAGAATGCTTGCACCAAGCTTGTCCAGGCAGCTCAGA TGCTTCAGTCAGACCCTTACTCAGTGCCTGCTCGAGATTATCTAATTGATGGGTCAAGGGGCATCCTCTC TGGAACATCAGACCTGCTCCTTACCTTCGATGAGGCTGAGGTCCGTAAAATTATTAGAGTTTGCAAAGGA ATTTTGGAATATCTTACAGTGGCAGAGGTGGTGGAGACTATGGAAGATTTGGTCACTTACACAAAGAATC TTGGGCCAGGAATGACTAAGATGGCCAAGATGATTGACGAGAGACAGCAGGAGCTCACTCACCAGGAGCA CCGAGTGATGTTGGTGAACTCGATGAACACCGTGAAAGAGTTGCTGCCAGTTCTCATTTCAGCTATGAAG ATTTTTGTAACAACTAAAAACTCAAAAAACCAAGGCATAGAGGAAGCTTTAAAAAATCGCAATTTTACTG TAGAAAAAATGAGTGCTGAAATTAATGAGATAATTCGTGTGTTACAACTCACCTCTTGGGATGAAGATGC CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCATTGGCCTCCATAGACTCCAAACTGAACCAGGCC AAAGGTTGGCTCCGTGACCCTAGTGCCTCCCCAGGGGATGCTGGTGAGCAGGCCATCAGACAGATCTTAG ATGAAGCTGGAAAAGTTGGTGAACTCTGTGCAGGCAAAGAACGCAGGGAGATTCTGGGAACTTGCAAAAT GCTAGGGCAGATGACTGATCAAGTGGCTGACCTCCGTGCCAGAGGACAAGGATCCTCACCGGTGGCCATG CAGAAAGCTCAGCAGGTATCTCAGGGTCTGGATGTGCTCACAGCAAAAGTGGAAAATGCAGCTCGCAAGC TGGAAGCCATGACCAACTCAAAGCAGAGCATTGCAAAGAAGATCGATGCTGCTCAGAACTGGCTTGCAGA TCCAAATGGTGGACCGGAAGGAGAAGAGCAGATTCGAGGTGCTTTGGCTGAAGCTCGGAAAATAGCAGAA TTATGTGATGATCCTAAAGAAAGAGATGACATTCTACGTTCCCTTGGGGAAATATCTGCTCTGACTTCTA AATTAGCAGATCTACGAAGACAGGGGAAAGGAGATTCTCCAGAGGCTCGAGCCTTGGCCAAACAGGTGGC CACGGCCCTGCAGAACCTGCAGACCAAAACCAACCGGGCTGTGGCCAACAGCAGACCGGCCAAAGCAGCT GTACACCTTGAGGGCAAGATTGAGCAAGCACAGCGGTGGATTGATAATCCCACAGTGGATGACCGTGGAG TCGGTCAGGCTGCCATCCGGGGGCTTGTGGCCGAAGGGCATCGTCTGGCTAATGTTATGATGGGGCCTTA TCGGCAAGATCTTCTCGCCAAGTGTGACCGAGTGGACCAGCTGACAGCCCAGCTGGCTGACCTGGCTGCC AGAGGGGAAGGGGAGAGTCCTCAGGCACGAGCACTTGCATCTCAGCTCCAAGACTCCTTAAAGGATCTAA AAGCTCGGATGCAGGAGGCCATGACTCAGGAAGTGTCAGATGTTTTCAGCGATACCACAACTCCCATCAA GCTGTTGGCAGTGGCAGCCACGGCGCCTCCTGATGCGCCTAACAGGGAAGAGGTATTTGATGAGAGGGCA GCTAACTTTGAAAACCATTCAGGAAAGCTTGGTGCTACGGCCGAGAAGGCGGCTGCGGTTGGTACTGCTA ATAAATCAACAGTGGAAGGCATTCAGGCCTCAGTGAAGACGGCCCGAGAACTCACACCCCAGGTGGTCTC GGCTGCTCGTATCTTACTTAGGAACCCTGGAAATCAAGCTGCTTATGAACATTTTGAGACCATGAAGAAC CAGTGGATCGATAATGTTGAAAAAATGACAGGGCTGGTGGACGAAGCCATTGATACCAAATCTCTGTTGG ATGCTTCAGAAGAAGCAATTAAAAAAGACCTGGACAAGTGCAAGGTAGCTATGGCCAACATTCAGCCTCA GATGCTGGTTGCTGGGGCAACCAGTATTGCTCGTCGGGCCAACCGGATCCTGCTGGTGGCTAAGAGGGAG GTGGAGAATTCCGAGGATCCCAAGTTCCGTGAGGCTGTGAAAGCTGCCTCTGATGAATTGAGCAAAACCA TCTCCCCGATGGTGATGGATGCAAAAGCTGTGGCTGGAAACATTTCCGACCCTGGACTGCAAAAGAGCTT CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAGGTCAGAGAAGCCTTCCAACCTCAGGAGCCT GACTTCCCGCCGCCTCCACCAGACCTTGAACAACTCCGACTAACAGATGAGCTTGCTCCTCCCAAACCAC CTCTGCCTGAAGGTGAGGTCCCTCCACCTAGGCCTCCACCACCAGAGGAAAAGGATGAAGAGTTCCCTGA GCAGAAGGCCGGGGAGGTGATTAACCAGCCAATGATGATGGCTGCCAGACAGCTCCATGATGAAGCTCGC AAATGGTCCAGCAAGCCGGGCATCCCAGCCGCTGAGGTGGGTATAGGTGTTGTAGCTGAGGCAGATGCGG CCGATGCTGCTGGCTTCCCTGTCCCCCCTGACATGGAAGACGATTACGAACCTGAGCTGCTGTTAATGCC ATCCAATCAGCCGGTCAACCAGCCCATTCTGGCCGCGGCTCAGTCCTTGCATCGGGAAGCTACCAAGTGG TCTAGTAAGGGCAATGACATCATTGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCTGAGATGTCTCGGC TGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTCATTCAGTGTGCCAAGGACATCGCCAAGGCCTCAGA TGAGGTGACTCGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAACGGATTAGAACCAACCTCTTA CAGGTATGTGAGCGAATCCCAACCATAAGCACCCAGCTCAAAATCCTGTCCACAGTGAAGGCCACCATGC TGGGCCGGACCAACATCAGTGATGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCACAATGCCCAGAA CCTCATGCAGTCTGTGAAGGAGACTGTGCGGGAAGCTGAAGCTGCTTCAATCAAAATTCGAACAGATGCT GGATTTACACTGCGCTGGGTTAGAAAGACTCCCTGGTACCAGTAG >OriGene 5' read for NM_014000 unedited
GGTTCAAATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCGGTTCC CGGCCCCGTGGATCCTACTTCTCTGTCGCCCGCGGTTCGCCGCCCCGCTCGCCGCCGCGA TGCCAGTGTTTCATACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCACAGCAGATCT CCCACCTGGTGATAATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCA CCGCGCCCGTGGCCGCCGTGCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAGA CTGTTCAAACCACTGAGGATCAGATTTTGAAGAGAGATATGCCACCAGCATTTATTAAGG TTGAGAATGCTTGCACCAAGCTTGTCCAGGCAGCTCAGATGCTTCAGTCAGACCCTTACT CAGTGCCTGCTCGAGATTATCTAATTGATGGGTCAAGGGGCATCCTCTCTGGAACATCAG ACCTGCTCCTTACCTTCGATGAGGCTGAGGTCCGTAAAATTATTAGAGTTTGCAAAGGAA TTTTGGAATATCTTACAGTGGCAGAGGTGGTGGAGACTATGGAAGATTTGGTCACTTACA CAAAGAATCTTGGGCCAGGAATGACTAAGATGGCCAAGATGATTGACGAGAGACAGCAGG AGCTCACTCACCAGGAGCACCGAGTGATGTTGGTGAACTCGATGAACACCCGTGAAAGAG TTGCTGCCAGTTCTCATTTCAGCTATGAAGATTTTTGGTACCACTATNAACTCCAAAAAC CCAAGGCCTAGNAGGAAGCTTTTTAAAAATCCGCATTTTTACTGTAGAANAAAATGAGTG CCTGAAATAATGAAATAATTCGGGGGTACAACTTACCTCTTGGATGAAGATCCTGGCCAC CAAGGCCCCTGACCATGANGAAGCTTTGCCTCCTAAACTCAAATGGACCGGC >OriGene 3' read for NM_014000 unedited
NNNCCGTCCTGNACCGCGGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTTAATTT GCTTCACTGTGTCTCGAGCACTGATTTGGAGAAAAGCACATCCGGCATAAAGTGTAAACC AGTGTCTCAAACCACTGGAAGAACCGGGAGAGCAAACATGATTTTTCTTATTTCCTCTAA GTAATCTTTCTTTAGTAAAACAACAAGTGATCTTTGGCATAGATTCATACTTTAAAGGCA TTAATATTGCATTTATATCAGGCAAGCAACTATACAAATATGCTGAGGGCCTTGAAAATA ATCATCCTCATTATAAAGGAAATAGTGAAAGCCTGAGTGTAAAGGACCAACTTAAGTTGT ACACATTCGATGTTGGGAACTAACACACAGCGATGGGTGGGAAGGAAGGATGTTCAGGCA AGGTTCTTACTCCTTTACTCATCTGGTTCTGGCTTTGGGAAAAAATAAGGTTTCATGTGC TGGGAAATACTTACCAGTAATAAGTACCAAACAGGAAACACTGCCCTCTCATTTTGCCTA GTAGGAACTTACTGTGGGGATAAGAAATCTGAACCCCCTTCCTCCCTCTGACCCCGTCCT TCGGGATCTNACGCCACATAGCATTGTTTCTCCTTGCGTCGCCGGGACGCCCACGCCTCA CCGCCTACCTTCCCCCCCCTCGCTTCCACCCTCAACCTCCCTTTCAAACCTCACTTCGTC ATTCACCCTCCCGCGCCCTTGCGCCACCTCTCCTCTCGATCCACTTATTCCTGCCCCGCG CTCTAGACTACCTTTGCTGAGTCCGTTATCTAGCTCTTGCTCGTCTCCTGCTCTCCTATA CCGCCTCCCTCCCGCCTCCACTCTCGTCCCTCTGCTCCCCTTGCCTTGCGTGCTTTTCGT CCGCCCTGCNACCAACCATAGCCACTTCCTACCCATATCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014000 |
| Insert Size | 5450 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014000.2, NP_054706.1 |
| RefSeq Size | 5647 bp |
| RefSeq ORF | 3405 bp |
| Locus ID | 7414 |
| UniProt ID | P18206 |
| Domains | Vinculin |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton |
| Gene Summary | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) differs from variant 2 in having an additional exon in the 3' coding region. It thus encodes a longer isoform (meta-VCL). |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219299 | VCL (Myc-DDK-tagged)-Human vinculin (VCL), transcript variant 1 |
CNY 8,688.00 |
|
| RC219299L3 | Lenti-ORF clone of VCL (Myc-DDK-tagged)-Human vinculin (VCL), transcript variant 1 |
CNY 13,210.00 |
|
| RC219299L4 | Lenti-ORF clone of VCL (mGFP-tagged)-Human vinculin (VCL), transcript variant 1 |
CNY 13,210.00 |
|
| RG219299 | VCL (tGFP-tagged) - Human vinculin (VCL), transcript variant 1 |
CNY 12,450.00 |
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