Rad6 (UBE2A) (NM_003336) Human Untagged Clone
CAT#: SC109859
UBE2A (untagged)-Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HHR6A; MRXS30; MRXSN; RAD6A; UBC2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC109859 sequence for NM_003336 edited (data generated by NextGen Sequencing)
ATGTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCT CCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATT TTCGGGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACT GAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAAT GTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTAT GATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGT CCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGT GTTTCTGCAATAGTAGAACAAAGCTGGCGTGATTGTTGA Clone variation with respect to NM_003336.2 >OriGene 5' read for NM_003336 unedited
GTCAAGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGACTTCGGA GAGGCAGCGCGGTTCCTCTGGGTGCTTCCGCCTCCCCTTCTCCTGCTTCTCCAGCCTCTT CGGCCTCCTCGCCCGCCGCGGGAACCCGAGACCCCAGTGTATGCCCCACCCCTGACCCCG CTCGCGACATGTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGG AGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACG CGGTCATTTTCGGGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAG AATTCACTGAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCC ATCCAAATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTC CAACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATC CCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACANACGGGAATATG AAAAGCGTGTTTCTGNCATAGTAGAACAAAGCTGGCGTGATTGTTGACCCCCGGTACAGT TTAAAGAAAGCTGCCCATAGAAAATATATATNTGATGTGTTGTCACCTCCCTACTNCTGT CATTACATTTACTTTATTAAAAGCAAAATAACTGGTGTGCTGTTTCCCATCTTCTTGCCA AGTTTTCCTACCCCTCTACCCTCTTCTTAACATCAGAAACAACCTCTTTGAAAATCAATG TACTGTACCCTGGTTACTCTGGCAAAAATAACTAAGGCTCCAGTTTTTNCGGGTGAATTC CATTTCCAAGTTTCCAGGCCGTTATTTTTATTATTGACCTTAAGGCTTT >OriGene 3' read for NM_003336 unedited
TTTTTTTTTTTTTTTATGAAATTTTAAGGTTATTTTTATTTACAACTTTTGAAAAAGTAC ATTTTTTTTTACATGGGTTACTTGTGCAAAGTTAGATTTGGAAGTGATAAATGCATAAAA GGTGACAATAGAACATTAGACAAAACATTTACAAGCCTTGTCCCATACTGCTACTTAAAG GTACTATATATCTAAAAGTATAAATATCCAAAAAAAGATCGCAGACATTGGCTTTAAGGT TCTCAGATGCTGAAAGGGAG |
Restriction Sites | NotI-NotI |
ACCN | NM_003336 |
Insert Size | 1820 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003336.2, NP_003327.2 |
RefSeq Size | 1799 bp |
RefSeq ORF | 459 bp |
Locus ID | 7319 |
UniProt ID | P49459 |
Domains | UBCc |
Protein Families | Druggable Genome |
Protein Pathways | Ubiquitin mediated proteolysis |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204194 | UBE2A (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1 |
CNY 1,200.00 |
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RC204194L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1, Myc-DDK-tagged |
CNY 3,600.00 |
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RC204194L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC204194L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204194L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG204194 | UBE2A (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 1 |
CNY 2,800.00 |