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TCP1 alpha (TCP1) (NM_030752) Human Untagged Clone
CAT＃: SC109713
TCP1 (untagged)-Human t-complex 1 (TCP1), transcript variant 1
CNY 4,152.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCT-alpha; CCT1; CCTa; D6S230E; TCP-1-alpha |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC109713 sequence for NM_030752 edited (data generated by NextGen Sequencing)
ATGGAGGGGCCTTTGTCCGTGTTCGGTGACCGCAGCACTGGGGAAACGATCCGCTCCCAA AACGTTATGGCTGCAGCTTCGATTGCCAATATTGTAAAAAGTTCTCTTGGTCCAGTTGGC TTGGATAAAATGTTGGTGGATGATATTGGTGATGTAACCATTACTAACGATGGTGCAACC ATCCTGAAGTTACTGGAGGTAGAACATCCTGCAGCTAAAGTTCTTTGTGAGCTGGCTGAT CTGCAAGACAAAGAAGTTGGAGATGGAACTACTTCAGTGGTTATTATTGCAGCAGAACTC CTAAAAAATGCAGATGAATTAGTCAAACAGAAAATTCATCCCACATCAGTTATTAGTGGC TATCGACTTGCTTGCAAGGAAGCAGTGCGTTATATCAATGAAAACCTAATTGTTAACACA GATGAACTGGGAAGAGATTGCCTGATTAATGCTGCTAAGACATCCATGTCTTCCAAAATC ATTGGAATAAATGGTGATTTCTTTGCTAACATGGTAGTAGATGCTGTACTTGCTATTAAA TACACAGACATAAGAGGCCAGCCACGCTATCCAGTCAACTCTGTTAATATTTTGAAAGCC CATGGGAGAAGTCAAATGGAGAGTATGCTCATCAGTGGCTATGCACTCAACTGTGTGGTG GGATCCCAGGGCATGCCCAAGAGAATCGTAAATGCAAAAATTGCTTGCCTTGACTTCAGC CTGCAAAAAACAAAAATGAAGCTTGGTGTACAGGTGGTCATTACAGACCCTGAAAAACTG GACCAAATTAGACAGAGAGAATCAGATATCACCAAGGAGAGAATTCAGAAGATCCTGGCA ACTGGTGCCAATGTTATTCTAACCACTGGTGGAATTGATGATATGTGTCTGAAGTATTTT GTGGAGGCTGGTGCTATGGCAGTTAGAAGAGTTTTAAAAAGGGACCTTAAACGCATTGCC AAAGCTTCTGGAGCAACTATTCTGTCAACCCTGGCCAATTTGGAAGGTGAAGAAACTTTT GAAGCTGCAATGTTGGGACAGGCAGAAGAAGTGGTACAGGAGAGAATTTGTGATGATGAG CTGATCTTAATCAAAAATACTAAGGCTCGTACGTCTGCATCGATTATCTTACGTGGGGCA AATGATTTCATGTGTGATGAGATGGAGCGCTCTTTACATGATGCACTTTGTGTAGTGAAG AGAGTTTTGGAGTCAAAATCTGTGGTTCCCGGTGGGGGTGCTGTAGAAGCAGCCCTTTCC ATATACCTTGAAAACTATGCAACCAGCATGGGGTCTCGGGAACAGCTTGCGATTGCAGAG TTTGCAAGATCACTTCTTGTTATTCCCAATACACTAGCAGTTAATGCTGCCCAGGACTCC ACAGATCTGGTTGCAAAATTAAGAGCTTTTCATAATGAGGCCCAGGTTAACCCAGAACGT AAAAATCTAAAATGGATTGGTCTTGATTTGAGCAATGGTAAACCTCGAGACAACAAACAA GCAGGGGTGTTTGAACCAACCATAGTTAAAGTTAAGAGTTTGAAATTTGCAACAGAAGCT GCAATCACCATTCTTCGAATTGATGATCTTATTAAATTACATCCAGAAAGTAAAGATGAT AAACATGGAAGTTATGAAGATGCTGTTCACTCTGGAGCCCTTAATGATTGA Clone variation with respect to NM_030752.2 >OriGene 5' read for NM_030752 unedited
ATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGGGCAGCCGGGAAGCGGGTGGG GTGGTGTGTTACCCAGTAGCTCCTGGGACATCGCTCGGGTACGCTCCACGCCGTCGCAGC CACTGCTGTGGTCGCCGGTCGGCCGAGGGGCCGCGATACTGGTTGCCCGCGGTGTAAGCA GAATTCGACGTGTATCGCTGCCGTCAAGATGGAGGGGCCTTTGTCCGTGTTCGGTGACCG CAGCACTGGGGAAACGATCCGCTCCCAAAACGTTATGGCTGCAGCTTCGATTGCCAATAT TGTAAAAAGTTCTCTTGGTCCAGTTGGCTTGGATAAAATGTTGGTGGATGATATTGGTGA TGTAACCATTACTAACGATGGTGCAACCATCCTGAAGTTACTGGAGGTAGAACATCCTGC AGCTAAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGGAACTAC TTCAGTGGTTATTATTGCAGCAGAACTCCTAAAAAATGCAGATGAATTAGTCAAACAGAA AATTCATCCCACATCAGTTATTAGTGGCTATCGACTTGCTTGCAAGGAAGCAGTGCGTTA TATCAATGAAAACCTAATTGTTAACACAGATGAACTGGGAAGAGATTGCCTGATTAATGC TGCTAAGACATCCATGTCTTCCAAATCATTGGATAAATGGTGATTTCTTTGCTAACATGT AGTAGATGCTGTACTTGCTATTAAATACACAGACATTAGAGGCCAGCCACGCTATCCAGT CAACTCTGTTAATATTNTGAAAGCCATGGGAGAGTCAAATGGAGAGTATGCTCATCAGTG GCTATGCACTCAACTGTGTGGGTGGGATCCCAGGGNCATGCCAGNAGATCGTAATGCAAA AATTGCTTGCCTTGACTTCAGCTGCAAAACAAAATGAAGCTGGNGTACAGTGGTCATTAC GACCTGANACT >OriGene 3' read for NM_030752 unedited
GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTACAGCTTGTACTTTACTTTA ATGTGTAATACTCAACTCAAGGTACAAGACAATTGCATTTAACATTGTTATAAATAAAAG GAACATCAGATCAATCATTAAGGGCTCCAGAGTGAACAGCATCTTCATAACTTCCATGTT TATCATCTTTACTTTCTGGATGTAATTTAATAAGATCATCAATTCGAAGAATGGTGATTG CAGCTTCTGTTGCAAATTTCAAACTCTTAACTTTAACTATGGTTGGTTCAAACACCCCTG CTTGTTTGTTGTCTCGAGGTTTACCATTGCTCAAATCAAGACCAATCCATTTTAGATTTT TACGTTCTGGGTTAACCTGGGCCTCATTATGAAAAGCTCTTAATTTTGCAACCAGATCTG TGGAGTCCTGGGCAGCATTAACTGCTAGTGTATTGGGAATAACAAGAAGTGATCTTGCAA ACTCTGCAATCGCAAGCTGTTCCCGAGACCCCATGCTGGTTGCATAGTTTTCAAGGTATA TGGAAAGGGCTGCTTCTACAGCACCCCCACCGGGAACCACAGATTTTGACTCCAAAACTC TCTTCACTACACAAAGTGCATCATGTAAAGAGCGCTCCATCTCATCACACATGAAATCAT TTGCCCCACGTAAGATAATCGATGCAGACGTACGAGCCTTAGTATTTTTGATTAAGATCA GCTCATCATCACAAATTCTCTCCTGTACCACTTCTTCTGCCTGTCCCACATTGCAGCTTC AAAAAGTTCTTCACCTTCCAATTGGCCAGGTTGACAGATAGTTGCTCCAGAAGCTTTGGC ATGCGTTTAAGGTCCTTTTTAAAACTCTTCTACTGCCATGCACCAGCCTCACAAATACTT CAGAACATATCATCATTCACCATGGGTAAAATACATGGCACAGTGGCAGATCTCTGAATC TCTCTGGGGAATCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_030752 |
| Insert Size | 1920 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030752.2, NP_110379.2 |
| RefSeq Size | 2463 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 6950 |
| UniProt ID | P17987 |
| Domains | cpn60_TCP1 |
| Gene Summary | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200686 | TCP1 (Myc-DDK-tagged)-Human t-complex 1 (TCP1), transcript variant 1 |
CNY 6,208.00 |
|
| RC200686L1 | Lenti ORF clone of Human t-complex 1 (TCP1), transcript variant 1, Myc-DDK-tagged |
CNY 8,608.00 |
|
| RC200686L2 | Lenti ORF clone of Human t-complex 1 (TCP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200686L3 | Lenti ORF clone of Human t-complex 1 (TCP1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200686L4 | Lenti ORF clone of Human t-complex 1 (TCP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200686 | TCP1 (tGFP-tagged) - Human t-complex 1 (TCP1), transcript variant 1 |
CNY 7,808.00 |
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