PLAT (NM_000931) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	T-PA; TPA
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_000931 edited GAATTCGGCACGAGGCATGGATGCAATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTG TGGAGCAGTCTTCGTTTCGCCCAGCCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAG ATCTTACCAAGTGATCTGCAGAGATGAAAAAACGCAGATGATATACCAGCAACATCAGTC ATGGCTGCGCCCTGTGCTCAGAAGCAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAG GGCACAGTGCCACTCAGTGCCTGTCAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGG CACCTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGC TGGGAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTA CAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGC GTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAA CCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCCCTGGTGCTACGTCTTTAAGGC GGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGAGGGAAACAGTGACTG CTACTTTGGGAATGGGTCAGCCTACCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTC CTGCCTCCCGTGGAATTCCATGATCCTGATAGGCAAGGTTTACACAGCACAGAACCCCAG TGCCCAGGCACTGGGCCTGGGCAAACATAATTACTGCCGGAATCCTGATGGGGATGCCAA GCCCTGGTGCCACGTGCTGAAGAACCGCAGGCTGACGTGGGAGTACTGTGATGTGCCCTC CTGCTCCACCTGCGGCCTGAGACAGTACAGCCAGCCTCAGTTTCGCATCAAAGGAGGGCT CTTCGCCGACATCGCCTCCCACCCCTGGCAGGCTGCCATCTTTGCCAAGCACAGGAGGTC GCCCGGAGAGCGGTTCCTGTGCGGGGGCATACTCATCAGCTCCTGCTGGATTCTCTCTGC CGCCCACTGCTTCCAGGAGAGGTTTCCGCCCCACCACCTGACGGTGATCTTGGGCAGAAC ATACCGGGTGGTCCCTGGCGAGGAGGAGCAGAAATTTGAAGTCGAAAAATACATTGTCCA TAAGGAATTCGATGATGACACTTACGACAATGACATTGCGCTGCTGCAGCTGAAATCGGA TTCGTCCCGCTGTGCCCAGGAGAGCAGCGTGGTCCGCACTGTGTGCCTTCCCCCGGCGGA CCTGCAGCTGCCGGACTGGACGGAGTGTGAGCTCTCCGGCTACGGCAAGCATGAGGCCTT GTCTCCTTTCTATTCGGAGCGGCTGAAGGAGGCTCATGTCAGACTGTACCCATCCAGCCG CTGCACATCACAACATTTACTTAACAGAACAGTCACCGACAACATGCTGTGTGCTGGAGA CACTCGGAGCGGCGGGCCCCAGGCAAACTTGCACGACGCCTGCCAGGGCGATTCGGGAGG CCCCCTGGTGTGTCTGAACGATGGCCGCATGACTTTGGTGGGCATCATCAGCTGGGGCCT GGGCTGTGGACAGAAGGATGTCCCGGGTGTGTACACCAAGGTTACCAACTACCTAGACTG GATTCGTGACAACATGCGACCGTGACCAGGAACACCCGACTCCTCAAAAGCAAATGAGAT CCCGCCTCTTCTTCTTCAGAAGACACTGCAAAGGCGCAGTGCTTCTCTACAGACTTCTCC AGACCCACCACACCGCAGAAGCGGGACGAGACCCTACAGGAGAGGGAAGAGTGCATTTTC CCAGATACTTCCCATTTTGGAAGTTTTCAGGACTTGGTCTGATTTCAGGATACTCTGTCA GATGGGAAGACATGAATGCACACTGGCCTCTCCAGGAATGCCTCCTCCCTGGGCAGAAAG TGGCCATGCCACCCTGTTTTCAGCTAAAGCCCAACCTCCTGACCTGTCACCGTGAGCAGC TTTGGAAACAGGACCACAAAAATGAAAGCATGTCTCAATAGTAAAAGATAACAAGATCTT TCAGGAAAGACGGATTGCATTAGAAATAGACAGTATATTTATAGTCACAAGAGCCCAGCA GGGCCTCAAAGTTGGGGCAGGCTGGCTGGCCCGTCATGTTCCTCAAAAGCACCCTTGACG TCAAGTCTCCTTCCCCTTTCCCCACTCCCTGGCTCTCAGAAGGTATTCCTTTTGTGTACA GTGTGTAAAGTGTAAATCCTTTTTCTTTATAAACTTTAGAGTAGCATGAGAGAATTGTAT CATTTGAACAACTAGGCTTCAGCATATTTATAGCAATCCATGTTAGTTTTTACTTTCTGT TGCCACAACCCTGTTTTATACTGTACTTAATAAATTCAGATATAAAAAAAAAAAAAAAAA AAAAAAAAACTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_000931 unedited TGTTCGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCATGGATGC AATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCAG CCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAGATCTTACCAAGTGATCTGCAGAGA TGAAAAAACGCAGATGATATACCAGCAACATCAGTCATGGCTGCGCCCTGTGCTCAGAAG CAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAGGGCACAGTGCCACTCAGTGCCTGT CAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGGCACCTGCCAGCAGGCCCTGTACTT CTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGCTGGGAAGTGCTGTGAAATAGATAC CAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTACAGGGGCACGTGGAGCACAGCGGA GAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGCGTTGGCCCAGAAGCCCTACAGCGG GCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAACCACAACTACTGCAGAAACCCAGA TCGAGACTCANAGCCCTGGTGCTACGTCTTTAAGGCGGNGAAGTACAGCTCAGAGTTCTG CAGCACCCCTGCCTGCTCTGNAGGAAACAGTGACTGCTACTTTGNGAATGGGTCAGCCTA CCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTCCTGCCTCCCGTGGAATNCATGATC CTGATAGGGCAGGGTTACACAGCACAGAACCCCAGTGCCCCAGCACTGGGCCTGGGGCAN ACATATTACTGCCGGNATNCTGATGGGGATGCCCAGCCCTGGTGCCACGTGCTGAAAAAC CGCAGCTGACGTGGNAGTACTGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_000931 unedited GTACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTATATCT GAATTTATTAAGTACAGTATAAAACAGGGTTGTGGCAACAGAAAGTAAAAACTAACATGG ATTGCTATAAATATGCTGAAGCCTAGTTGTTCAAATGATACAATTCTCTCATGCTACTCT AAAGTTTATAAAGAAAAAGGATTTACACTTTACACACTGTACACAAAAGGAATACCTTCT GAGAGCCAGGGAGTGGGGAAAGGGGAAGGAGACTTGACGTCAAGGGTGCTTTTGAGGAAC ATGACGGGCCAGCCAGCCTGCCCCAACTTTGAGGCCCTGCTGGGCTCTTGTGACTATAAA TATACTGTCTATTTCTAATGCAATCCGTCTTTCCTGAAAGATCTTGTTATCTTTTACTAT TGAGACATGCTTTCATTTTTGTGGTCCTGTTTCCAAAGCTGCTCACGGTGACAGGTCAGG AGGTTGGGCTTTAGCTGAAAACAGGGTGGCATGGCCACTTTCTGCCCAGGGAGGAGGCAT TCCTGGAGAGGCCAGTGTGCATTCATGTCTTCCCATCTGACAGAGTATCCTGAAATCAGA CCAAGTCCTGAAAACTTCCCAAATGGGAAGTATCTGGGAAAATGCCCTCTTCCCTTTCCT GTAAGGGCTCGCCCCGCTTTTGCGGTGTGGTGGGCTTGAAGAATCCTGTTACAAAACCTC GCCCCTTTGCCATGCTTTCTGAACAACAAAAGGCGGATCTCCATTGCTTTTGAAGAGCTC GGCGTTCCCGGCAACGGCCCCTCGTCCCACCATCCCTCTAAGGACTTGGTCACCTTGGGC CCCCCCCGGACACCTTCTGCCACACTCAGCTCACCTGTCGTAGCCCCCCAACCTGCGCTC TCGTCCAACCACCCGGGCTCCATCCACAC
Restriction Sites	NotI-NotI
ACCN	NM_000931
Insert Size	2600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_000931.1, NP_000922.1
RefSeq Size	2461 bp
RefSeq ORF	2160 bp
Locus ID	5327
Domains	KR, FN1, EGF
Protein Families	Druggable Genome, Protease, Secreted Protein
Protein Pathways	Complement and coagulation cascades
Gene Summary	This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (2) lacks exons 9, 10, and 11, and uses an alternate splice acceptor site in exon 12 resulting in a frameshift, as compared to variant 1. It encodes isoform 2 which has a distinct C-terminus as compared to the other isoforms and likely does not form a two chain disulfide linked protein like isoform 1.

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