United States
Germany
Japan
United Kingdom
China
Integrin beta 4 binding protein (EIF6) (NM_002212) Human Untagged Clone
CAT＃: SC109359
EIF6 (untagged)-Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | b(2)gcn; CAB; eIF-6; EIF3A; ITGB4BP; p27(BBP); p27BBP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC109359 sequence for NM_002212 edited (data generated by NextGen Sequencing)
ATGGCGGTCCGAGCTTCGTTCGAGAACAACTGTGAGATCGGCTGCTTTGCCAAGCTCACC AACACCTACTGTCTGGTAGCGATCGGAGGCTCAGAGAACTTCTACAGTGTGTTCGAGGGC GAGCTCTCCGATACCATCCCCGTGGTGCACGCGTCTATCGCCGGCTGCCGCATCATCGGG CGCATGTGTGTGGGGAACAGGCACGGTCTCCTGGTACCCAACAATACCACCGACCAGGAG CTGCAACACATTCGCAACAGCCTCCCAGACACAGTGCAGATTAGGCGGGTGGAGGAGCGG CTCTCAGCCTTGGGCAATGTCACCACCTGCAATGACTACGTGGCCTTGGTCCACCCAGAC TTGGACAGGGAGACAGAAGAAATTCTGGCAGATGTGCTCAAGGTGGAAGTCTTCAGACAG ACAGTGGCCGACCAGGTGCTAGTAGGAAGCTACTGTGTCTTCAGCAATCAGGGAGGGCTG GTGCATCCCAAGACTTCAATTGAAGACCAGGATGAGCTGTCCTCTCTTCTTCAAGTCCCC CTTGTGGCGGGGACTGTGAACCGAGGCAGTGAGGTGATTGCTGCTGGGATGGTGGTGAAT GACTGGTGTGCCTTCTGTGGCCTGGACACAACCAGCACAGAGCTGTCAGTGGTGGAGAGT GTCTTCAAGCTGAATGAAGCCCAGCCTAGCACCATTGCCACCAGCATGCGGGATTCCCTC ATTGACAGCCTCACCTGA Clone variation with respect to NM_002212.2 >OriGene 5' read for NM_002212 unedited
ACTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCGCGAGGGAGCTTGTTA CTGGTTACTTGGCCTCATGGCGGTCCGAGCTTCGTTCGAGAACAACTGTGAGATCGGCTG CTTTGCCAAGCTCACCAACACCTACTGTCTGGTAGCGATCGGAGGCTCAGAGAACTTCTA CAGTGTGTTCGAGGGCGAGCTCTCCGATACCATCCCCGTGGTGCACGCGTCTATCGCCGG CTGCCGCATCATCGGGCGCATGTGTGTGGGGAACAGGCACGGTCTCCTGGTACCCAACAA TACCACCGACCAGGAGCTGCAACACATTCGCAACAGCCTCCCAGACACAGTGCAGATTAG GCGGGTGGAGGAGCGGCTCTCAGCCTTGGGCAATGTCACCACCTGCAATGACTACGTGGC CTTGGTCCACCCAGACTTGGACAGGGAGACAGAAGAAATTCTGGCAGATGTGCTCAAGGT GGAAGTCTTCAGACAGACAGTGGCCGACCAGGTGCTAGTAGGAAGCTACTGTGTCTTCAG CAATCAGGGAGGGCTGGTGCATCCCAAGACTTCAATTGAAGACCAGGATGAGCTGTCCTC TCTTCTTCAAGTCCCCCTTGTGGCGGGGACTGTGAACCGAGGCAGTGAGGTGATTGCTGC TGGGATGGTGGTGAATGACTGGTGTGCCTTCTGTGGCCTGGACACACCCAGCACAGAGCT GTCAGTGGTGGAGAGTGTCTTCAAGCTGAATGAAGCCCAACCTAGCACCATTGCCACCAG CATGCGGGATTCCCTCATTGACAGCCTTCCCTGAGTCACCTTTCCAAGTTGTTCCATGGG CCTCCTGGCTTCTGGACTGTGGCCAACCTTTCTCCACATTCCGCCCATCTGTACCGNATG CTGTCAGGAAGGTGGCAGAAAGCCACCTGGACTGAGGGGCCTGGCACCCACCCTTTCC >OriGene 3' read for NM_002212 unedited
NCCCTTAGCTCTGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTGC CCGGNAGGAACTGCACTTTAATGGGGTGGCACAGGACAGCAGAACCCTCAGCCAGCAGCC ACAGGGCCTGCCAGCCAGCACAACAGAGCAGGTTTTTGCAGTAATGATAGATCCAGGCGA TAAGCACAGGTGGAAAAGGGTTGGGTGCCCAGCCCCTCAGTCCCAGTGAGCTCTCTGCCA CCTCCCTGCCAGCATCCGGTACAGATTGGGCGGAATGTGGAGAAGGTTGGCCACAGTCCA GAGCCAGGAGCCCATGGAACAACTTGGAAGGTGACTCAGGTGAGGCTGTCAATGAGGGAA TCCCGCATGCTGGTGGCAATGGTGCTAGGCTGGGCTTCATTCAGCTTGAAGACACTCTCC ACCACTGACAGCTCTGTGCTGGTTGTGTCCAGGCCACAGAAGGCACACCAGTCATTCACC ACCATCCCAGCAGCAATCACCTCACTGCCTCGGTTCACAGTCCCCGCCACAAGGGGGACT TGAAGAAGAGAGGACAGCTCATCCTGGTCTTCAATTGAAGTCTTGGGATGCACCAGCCCT CCCTGATTGCTGAAGACACAGTAGCTTCCTACTAGCACCTGGTCGGCCACTGTCTGTCTG AAGACTTCCACCTTGAGCACATCTGCCAGAATTTCTTCTGTCTCCCTGTCCAAGTCTGGG TGGACCAAGGCCACGTAGTCATTGCAGGTGGTGACATTGCCCAAGGCTGAGAGCCGCTCC TCCACCGCCTATCTGCCTGTGTCTGGGAGGCCTGTGCGAATGTGNTGCAGCTCCTGGTCG GCGGTATTTGGTGGGTACCAGNAGACCGTGCCTGTTCCCCACACACATGCGCCCCGAGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002212 |
| Insert Size | 1000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002212.2, NP_002203.1 |
| RefSeq Size | 1114 bp |
| RefSeq ORF | 738 bp |
| Locus ID | 3692 |
| UniProt ID | P56537 |
| Domains | eIF6 |
| Protein Families | Druggable Genome |
| Gene Summary | Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, and 6 all encode isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200568 | EIF6 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1 |
CNY 3,600.00 |
|
| RC200568L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC200568L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200568L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200568L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200568 | EIF6 (tGFP-tagged) - Human eukaryotic translation initiation factor 6 (EIF6), transcript variant 1 |
CNY 5,200.00 |
其它Integrin beta 4 binding protein产品
