Fibulin 1 (FBLN1) (NM_001996) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FBLN; FIBL1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001996, the custom clone sequence may differ by one or more nucleotides ATGGAGCGCGCCGCGCCGTCGCGCCGGGTCCCGCTTCCGCTGCTGCTGCTCGGCGGCCTTGCGCTGCTGG CGGCCGGAGTGGACGCGGATGTCCTCCTGGAGGCCTGCTGTGCGGACGGACACCGGATGGCCACTCATCA GAAGGACTGCTCGCTGCCATATGCTACGGAATCCAAAGAATGCAGGATGGTGCAGGAGCAGTGCTGCCAC AGCCAGCTGGAGGAGCTGCACTGTGCCACGGGCATCAGCCTGGCCAACGAGCAGGACCGCTGTGCCACGC CCCACGGTGACAACGCCAGCCTGGAGGCCACATTTGTGAAGAGGTGCTGCCATTGCTGTCTGCTGGGGAG GGCGGCCCAGGCCCAGGGCCAGAGCTGCGAGTACAGCCTCATGGTTGGCTACCAGTGTGGACAGGTCTTC CGGGCATGCTGTGTCAAGAGCCAGGAGACCGGAGATTTGGATGTCGGGGGCCTCCAAGAAACGGATAAGA TCATTGAGGTTGAGGAGGAACAAGAGGACCCATATCTGAATGACCGCTGCCGAGGAGGCGGGCCCTGCAA GCAGCAGTGCCGAGACACGGGTGACGAGGTGGTCTGCTCCTGCTTCGTGGGCTACCAGCTGCTGTCTGAT GGTGTCTCCTGTGAAGATGTCAATGAATGCATCACGGGCAGCCACAGCTGCCGGCTTGGAGAATCCTGCA TCAACACAGTGGGCTCTTTCCGCTGCCAGCGGGACAGCAGCTGCGGGACTGGCTATGAGCTCACAGAGGA CAATAGCTGCAAAGATATTGACGAGTGTGAGAGTGGTATTCATAACTGCCTCCCCGATTTTATCTGTCAG AATACTCTGGGATCCTTCCGCTGCCGACCCAAGCTACAGTGCAAGAGTGGCTTTATACAAGATGCTCTAG GCAACTGTATTGATATCAATGAGTGTTTGAGTATCAGTGCCCCGTGCCCTATCGGGCATACATGCATCAA CACAGAGGGCTCCTACACGTGCCAGAAGAACGTGCCCAACTGTGGCCGTGGCTACCATCTCAACGAGGAG GGAACGCGCTGTGTTGATGTGGACGAGTGCGCGCCACCTGCTGAGCCCTGTGGGAAGGGACATCGCTGCG TGAACTCTCCCGGCAGTTTCCGCTGCGAATGCAAGACGGGTTACTATTTTGACGGCATCAGCAGGATGTG TGTCGATGTCAACGAGTGCCAGCGCTACCCCGGGCGCCTGTGTGGCCACAAGTGCGAGAACACGCTGGGC TCCTACCTCTGCAGCTGTTCCGTGGGCTTCCGGCTCTCTGTGGATGGCAGGTCATGTGAAGACATCAATG AGTGCAGCAGCAGCCCCTGTAGCCAGGAGTGTGCCAACGTCTACGGCTCCTACCAGTGTTACTGCCGGCG AGGCTACCAGCTCAGCGATGTGGATGGAGTCACCTGTGAAGACATCGACGAGTGCGCCCTGCCCACCGGG GGCCACATCTGCTCCTACCGCTGCATCAACATCCCTGGAAGCTTCCAGTGCAGCTGCCCCTCGTCTGGCT ACAGGCTGGCCCCCAATGGCCGCAACTGCCAAGACATTGATGAGTGTGTGACTGGCATCCACAACTGCTC CATCAACGAGACCTGCTTCAACATCCAGGGCGGCTTCCGCTGCCTGGCCTTCGAGTGCCCTGAGAACTAC CGCCGCTCCGCAGCCACCCGCTGTGAGCGCTTGCCTTGCCATGAGAATCGGGAGTGCTCCAAGCTGCCTC TGAGAATAACCTACTACCACCTCTCTTTCCCCACCAACATCCAAGCGCCCGCGGTGGTTTTCCGCATGGG CCCCTCCAGTGCTGTCCCCGGGGACAGCATGCAGCTGGCCATCACCGGCGGCAATGAGGAGGGCTTTTTC ACCACCCGGAAGGTGAGCCCCCACAGTGGGGTGGTGGCCCTCACCAAGCCTGTCCCCGAGCCCAGGGACT TGCTCCTGACCGTCAAGATGGATCTCTCTCGCCACGGCACCGTCAGCTCCTTTGTGGCCAAGCTTTTCAT CTTTGTGTCTGCAGAGCTCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_001996 unedited NGTTACCATTTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGCCCACCGCC CGTCGCCCGCCGCCCATGNAGCGCGCCGCGCCGTCGCGCCGGGTCCCGCTTCCGCTGCTG CTGCTCGGCGGCCTTGCGCTGCTGGCGGCCGGAGTGGACGCGGATGTCCTCCTGGAGGCC TGCTGTGCGGACGGACACCGGATGGCCACTCATCAGAAGGACTGCTCGCTGCCATATGCT ACGGAATCCAAAGAATGCAGGATGGTGCAGGAGCAGTGCTGCCACAGCCAGCTGGAGGAG CTGCACTGTGCCACGGGCATCAGCCTGGCCAACGAGCAGGACCGCTGTGCCACGCCCCAC GGTGACAACGCCAGCCTGGAGGCCACATTTGTGAAGAGGTGCTGCCATTGCTGTCTGCTG GGGAGGGCGGCCCAGGCCCAGGGCCAGAGCTGCGAGTACAGCCTCATGGTTGGCTACCAG TGTGGACAGGTCTTCCGGGCATGCTGTGTCAAGAGCCAGGAGACCGGAGATTTGGATGTC GGGGGCCTCCAAGAAACGGATAAGATCATTGAGGTTGAGGAGGAACAAGAGGACCCATAT CTGAATGACCGCTGCCGAGGCGGGCCCTGCAAGCAGCAGTGCCGAGACACGGGTGACGAG GTGGTCTGCTCCTGCTTCGTGGGCTACCAGCTGCTGTCTGATGGTGTCTCCTGTGAAGAT GTCAATGAATGCATCACGGGCAGCCACAGCTGCCGGCTTGGAGAATCCTGCATCAACACA GTGGGCTCTTTCCGCTGCCAGCGGGACAGCAGCTGCGGGACTGGCTATGAGCTCACAGAG GACAATAGCTGCNAAGATATGACGAGTGTGAGAGTGGTATTCATAACTGCCTNCCCGATT TNATCTGTCAGATACTCTGGGATCCTTNCGCTGCCGACCCAGCTACAGTGCAGAGTGGCT TATACAAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_001996 unedited CCGCGTATTGTGGGCGCTGCTAATTTTATTAAGCCCCGGAGGCAGGTTAGGAAAGCAACA GAGGGAGACCCCGCGACGCGAAGCGAGTGCTCAGAGCTCTGCAGACACAAAGATGAAAAG CTTGGCCACAAAGGAGCTGACGGTGCCGTGGCGAGAGAGATCCATCTTGACGGTCAGGAG CAAGTCCCTGGGCTCGGGGACAGGCTTGGTGAGGGCCACCACCCCACTGTGGGGGCTCAC CTTCCGGGTGGTGAAAAAGCCCTCCTCATTGCCGCCGGTGATGGCCAGCTGCATGCTGTC CCCGGGGACAGCACTGGAGGGGCCCATGCGGAAAACCACCGCGGGCGCTTGGATGTTGGT GGGGAAAGAGAGGTGGTAGTAGGTTATTCTCAGAGGCAGCTTGGAGCACTCCCGATTCTC ATGGCAAGGCAAGCGCTCACAGCGGGTGGCTGCGGAGCGGCGGTAGTTCTCAGGGCACTC GAAGGCCAGGCAGCGGAAGCCGCCCTGGATGTTGAAGCAGGTCTCGTTGATGGAGCAGTT GTGGATGCCAGTCACACACTCATCAATGTCTTGGCAGTTGCGGCCATTGGGGGCCAGCCT GTAGCCAGACGAGGGGCAGCTGCACTGGAAGCTTCCAGGGATGTTGATGCAACGGTAGGA GCAGATGTGGCCCCCGGTGGGCAAGGCGCACTCGTCGATGTCTTCACAGGTGACTCCATT CAAATCGCTGAGCTGGTAGCCTCCGCGGCAGTTAACACTTGGTGTGAGCCGTAAACGTTG GCACACTTCTGGTTACCGGGGCTGTTGCTGCACCCCTTGTTGTTTTTACACGACCCGGCT TCCACAGAAAGCCGCAACCCCTTGTACATT
Restriction Sites	NotI-NotI
ACCN	NM_001996
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001996.2, NP_001987.2
RefSeq Size	2312 bp
RefSeq ORF	2052 bp
Locus ID	2192
UniProt ID	P23142
Domains	ANATO, EGF_CA, EGF, EGF
Protein Families	Secreted Protein
Gene Summary	Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (C) has an alternate 3' sequence, as compared to variant D. The encoded isoform C is shorter and has a distinct C-terminus, as compared to isoform D.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.