MED25 (NM_030973) Human Untagged Clone
CAT#: SC108991
MED25 (untagged)-Human mediator complex subunit 25 (MED25)
CNY 5,480.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2; TCBAP0758 |
Vector | pCMV6-XL6 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_030973, the custom clone sequence may differ by one or more nucleotides
ATGGTCCCCGGGTCCGAGGGCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGG GTACGGCCAACCTGGGACCCTACTTCGAGGGGCTCCGCAAGCACTACCTGCTCCCGGCCATCGAGTATTT TAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACAGCCTCGTGGTG TTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTCCCACCAGCAGCGCCTATGAGT TTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGGGTGGTGAGAGCTGCAGCCTCATCGCGGAAGG ACTCAGCACAGCCTTGCAGCTGTTTGATGACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGG GTCTGCCTCCTCATCTGCAACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGAT GCACAACTGAGAATCTTGTGCAGCAGATTGGGGAGCGGGGGATCCACTTCTCCATTGTGTCTCCCCGGAA GCTGCCTGCGCTTCGGCTTCTGTTTGAGAAGGCAGCCCCCCCGGCCTTGCTGGAGCCGCTGCAGCCTCCG ACAGATGTGAGCCAGGACCCGAGGCACATGGTGCTGGTTCGGGGACTCGTGCTGCCTGTTGGGGGTGGCT CAGCCCCAGGCCCCCTCCAGTCAAAGCAGCCAGTCCCCCTGCCTCCCGCCGCACCCTCAGGTGCCACTCT CTCAGCAGCCCCCCAGCAGCCTCTGCCCCCCGTCCCCCCGCAGTACCAGGTTCCCGGGAACCTGAGTGCA GCTCAGGTGGCCGCGCAGAATGCAGTGGAGGCTGCCAAGAACCAGAAGGCTGGGCTGGGCCCTCGCTTCT CGCCCATCACCCCTCTCCAACAAGCTGCTCCCGGAGTGGGTCCCCCCTTCAGCCAGGCCCCAGCTCCCCA ACTACCCCCAGGACCCCCTGGCGCCCCCAAGCCACCACCTGCTTCCCAGCCCAGTCTGGTCTCCACTGTG GCCCCTGGCTCCGGCCTGGCTCCCACGGCACAGCCCGGGGCACCGTCCATGGCAGGCACTGTGGCCCCAG GAGGGGTGAGCGGCCCTTCCCCAGCCCAGCTGGGAGCCCCAGCCCTCGGTGGGCAGCAGTCAGTCTCCAA TAAGCTTCTGGCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATGCCAAC ACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACCTGAAGACGGAGCAGT GGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGACCACCCTGGGCCCTTTGTTCCGGAA CTCAAGGATGGTCCAGTTCCATTTCACCAACAAGGACCTGGAGTCTCTCAAAGGCCTCTACCGCATCATG GGCAACGGCTTCGCGGGCTGCGTGCACTTCCCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCC TGTACTCGTCCAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAACGGCAT CCGGCAGGTCATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAGCAGCAGCGAGGAATGGGG GGACAGCAGGCACCCCCAGGGCTGGGGCCCATTCTGGAGGACCAAGCCAGGCCCTCACAGAATCTGCTCC AGCTCCGCCCACCGCAGCCCCAGCCTCAGGGTACCGTAGGGGCCTCTGGGGCCACGGGGCAGCCCCAGCC CCAAGGTACTGCCCAGCCCCCGCCAGGTGCCCCTCAAGGCCCTCCTGGAGCAGCTTCTGGCCCACCCCCT CCTGGACCCATCCTTCGGCCCCAGAACCCTGGGGCCAACCCTCAGCTGCGAAGCCTCCTCCTCAACCCAC CACCGCCGCAGACTGGGGTGCCCCCACCCCAGGCCTCCCTCCACCACCTCCAGCCACCAGGGGCTCCTGC GCTGCTGCCTCCGCCGCACCAGGGCCTGGGGCAGCCCCAGTTGGGGCCCCCACTCCTGCATCCACCACCT GCCCAGTCCTGGCCCGCACAACTTCCCCCTCGGGCTCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTC CCCGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGATCTCAT CTGA >OriGene 5' read for NM_030973 unedited
TATAACACCCGCCCTGTTGAACGCAAATGGGCGGTAGGCGTGTACGGTGGGCAGGTCTAT ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG GCCGCGAATTCGGCACGAGGGTGGCGGGTACCGCACGGGGTATGGTCCCCGGGTCCGAGG GCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGGGTACGGCCA ACCTGGGACCCTACTTCGAGGGGCTCCGCACGCACTACCTGCTCCCGGCCATCGAGTATT TTAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACA GCCTCGTGGTGTTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTC CCACCAGCAGCGCCTATGAGTTTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGG GTGGTGAGAGCTGCAGCCTCATCGCGGAAGGACTCAGCACAGCCTTGCAGCTGTTTGATG ACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGGGTCTGCCTCCTCATCTGCA ACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGATGCACAACTG AGCATCTTGTGCAGCAGATTGGGGAGCGGNGGCACCACTTCTCCATTGNGTCTCCCCGCA GCTGCCTGCGCTTTCGCTTCTGATTGAGAGGCAGCCCCCTCGCCTTGCTGAGCGCTGCAC GCTCCGAAGATGTAGCTGGACCTGAGACAGGGGCTTGTTTGGGACTCGGCTGCTGTTGGG GGGGTTTAACCTAGGCCCTTTAGTAAGGAGCCGGCCCTTGCTTCCCCGCACCTAGGGGCA TTTTTTAAAGCA >OriGene 3' read for NM_030973 unedited
TTTCCCTTGGGAATTAACTATGNACCCGCGGCCGCTTNCTANGATCGAGTTTTTTTTTTT TTTTTTTATCATCAAATCGGATCTTTACTCAACTATGGTGAATGCATGGAACATAATACA AACATGGATGTTACTGGAGTTTATATTTTTAAATTCAAAATTGAAAAAATACAAATTGAA AAATAGAAATATTTAATTATTAAAATTTTCTCTTTCATAATTCTTTTTGCGCCTTTGAGT TGAAGCCAAAAGGCTGGCGTCTTCCCTTCCACACCGAGATGTCAGTGTCAGGCCTCGGGT GGCACGGTGGGGACGGGCAGTTTAGAATTCAGCCTTGAATCCTTCAACTGGGAACGATGT TTAATTTGATTCAGGTTCGTAACGGAAAACAGCTGTTCACAAGCGTGGGTACTCCCAAAC ACAGATATTCAGGACAAACTACTGGAGATAGTTGTAGAATCTCACCATCTTCACACAGAC TTGTATTTAGGAACCAGCGCCGTATTGCACTGTAGTTCAATTACTATTTATGGTCTGCAT TCACACAAGAAACATCTGCCAACAAAGGCAAACTGGACAGTGTCTGTTCCTTCTCATGAG TCTGGATTCCGAGAGCCTTTGGGGGAATTTGGTCTTCAACCCCAGGACGTAGGTGAATAC AGTCCGGGCCACCTCTTTCATTTCTGAAAGCCGACTGCAGCACAGGAAAGAGTAAAGGTT TTTTTTCCTTGCCGGTGGATTCCCCAGGACCAAGCATACACCATCATACATTCAAGTAGC TGTTTAGGGACATCCTTGCGGAGAAATATTCAGTGTTCAGAACGGTCATAACCTTGACCA GAAGTCTTTGA |
Restriction Sites | NotI-NotI |
ACCN | NM_030973 |
Insert Size | 4700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_030973.2, NP_112235.2 |
RefSeq Size | 2335 bp |
RefSeq ORF | 2244 bp |
Locus ID | 81857 |
UniProt ID | Q71SY5 |
Gene Summary | This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010] |
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Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209044 | MED25 (Myc-DDK-tagged)-Human mediator complex subunit 25 (MED25) |
CNY 5,472.00 |
|
RC209044L1 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), Myc-DDK-tagged |
CNY 7,872.00 |
|
RC209044L2 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), mGFP tagged |
CNY 7,700.00 |
|
RC209044L3 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC209044L4 | Lenti ORF clone of Human mediator complex subunit 25 (MED25), mGFP tagged |
CNY 7,700.00 |
|
RG209044 | MED25 (tGFP-tagged) - Human mediator complex subunit 25 (MED25) |
CNY 6,370.00 |
|
SC321957 | MED25 (untagged)-Human mediator complex subunit 25 (MED25) |
CNY 5,480.00 |