MED25 (NM_030973) Human Untagged Clone

CAT#: SC108991

MED25 (untagged)-Human mediator complex subunit 25 (MED25)



  "NM_030973" in other vectors (7)

CNY 5,480.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-MED25 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACID1; ARC92; BVSYS; CMT2B2; P78; PTOV2; TCBAP0758
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_030973, the custom clone sequence may differ by one or more nucleotides


ATGGTCCCCGGGTCCGAGGGCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGG
GTACGGCCAACCTGGGACCCTACTTCGAGGGGCTCCGCAAGCACTACCTGCTCCCGGCCATCGAGTATTT
TAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACAGCCTCGTGGTG
TTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTCCCACCAGCAGCGCCTATGAGT
TTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGGGTGGTGAGAGCTGCAGCCTCATCGCGGAAGG
ACTCAGCACAGCCTTGCAGCTGTTTGATGACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGG
GTCTGCCTCCTCATCTGCAACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGAT
GCACAACTGAGAATCTTGTGCAGCAGATTGGGGAGCGGGGGATCCACTTCTCCATTGTGTCTCCCCGGAA
GCTGCCTGCGCTTCGGCTTCTGTTTGAGAAGGCAGCCCCCCCGGCCTTGCTGGAGCCGCTGCAGCCTCCG
ACAGATGTGAGCCAGGACCCGAGGCACATGGTGCTGGTTCGGGGACTCGTGCTGCCTGTTGGGGGTGGCT
CAGCCCCAGGCCCCCTCCAGTCAAAGCAGCCAGTCCCCCTGCCTCCCGCCGCACCCTCAGGTGCCACTCT
CTCAGCAGCCCCCCAGCAGCCTCTGCCCCCCGTCCCCCCGCAGTACCAGGTTCCCGGGAACCTGAGTGCA
GCTCAGGTGGCCGCGCAGAATGCAGTGGAGGCTGCCAAGAACCAGAAGGCTGGGCTGGGCCCTCGCTTCT
CGCCCATCACCCCTCTCCAACAAGCTGCTCCCGGAGTGGGTCCCCCCTTCAGCCAGGCCCCAGCTCCCCA
ACTACCCCCAGGACCCCCTGGCGCCCCCAAGCCACCACCTGCTTCCCAGCCCAGTCTGGTCTCCACTGTG
GCCCCTGGCTCCGGCCTGGCTCCCACGGCACAGCCCGGGGCACCGTCCATGGCAGGCACTGTGGCCCCAG
GAGGGGTGAGCGGCCCTTCCCCAGCCCAGCTGGGAGCCCCAGCCCTCGGTGGGCAGCAGTCAGTCTCCAA
TAAGCTTCTGGCCTGGAGCGGGGTCCTGGAGTGGCAAGAGAAACCCAAACCTGCCTCAGTGGATGCCAAC
ACCAAGCTGACGCGGTCACTGCCCTGCCAGGTCTACGTGAATCATGGCGAGAACCTGAAGACGGAGCAGT
GGCCCCAGAAGCTGATCATGCAGCTCATCCCCCAGCAGCTGCTGACCACCCTGGGCCCTTTGTTCCGGAA
CTCAAGGATGGTCCAGTTCCATTTCACCAACAAGGACCTGGAGTCTCTCAAAGGCCTCTACCGCATCATG
GGCAACGGCTTCGCGGGCTGCGTGCACTTCCCCCACACGGCGCCCTGTGAGGTGCGCGTGCTCATGCTCC
TGTACTCGTCCAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAACGGCAT
CCGGCAGGTCATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAGCAGCAGCGAGGAATGGGG
GGACAGCAGGCACCCCCAGGGCTGGGGCCCATTCTGGAGGACCAAGCCAGGCCCTCACAGAATCTGCTCC
AGCTCCGCCCACCGCAGCCCCAGCCTCAGGGTACCGTAGGGGCCTCTGGGGCCACGGGGCAGCCCCAGCC
CCAAGGTACTGCCCAGCCCCCGCCAGGTGCCCCTCAAGGCCCTCCTGGAGCAGCTTCTGGCCCACCCCCT
CCTGGACCCATCCTTCGGCCCCAGAACCCTGGGGCCAACCCTCAGCTGCGAAGCCTCCTCCTCAACCCAC
CACCGCCGCAGACTGGGGTGCCCCCACCCCAGGCCTCCCTCCACCACCTCCAGCCACCAGGGGCTCCTGC
GCTGCTGCCTCCGCCGCACCAGGGCCTGGGGCAGCCCCAGTTGGGGCCCCCACTCCTGCATCCACCACCT
GCCCAGTCCTGGCCCGCACAACTTCCCCCTCGGGCTCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTC
CCCGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGATCTCAT
CTGA


>OriGene 5' read for NM_030973 unedited
TATAACACCCGCCCTGTTGAACGCAAATGGGCGGTAGGCGTGTACGGTGGGCAGGTCTAT
ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
GCCGCGAATTCGGCACGAGGGTGGCGGGTACCGCACGGGGTATGGTCCCCGGGTCCGAGG
GCCCGGCCCGCGCCGGGAGCGTGGTGGCCGACGTGGTGTTTGTGATTGAGGGTACGGCCA
ACCTGGGACCCTACTTCGAGGGGCTCCGCACGCACTACCTGCTCCCGGCCATCGAGTATT
TTAATGGTGGTCCTCCTGCTGAGACGGACTTCGGGGGAGACTATGGGGGGACCCAGTACA
GCCTCGTGGTGTTCAACACAGTGGACTGCGCTCCCGAGTCCTACGTACAATGTCACGCTC
CCACCAGCAGCGCCTATGAGTTTGTCACCTGGCTCGATGGCATTAAGTTCATGGGCGGGG
GTGGTGAGAGCTGCAGCCTCATCGCGGAAGGACTCAGCACAGCCTTGCAGCTGTTTGATG
ACTTCAAGAAGATGCGCGAGCAGATTGGCCAGACGCACCGGGTCTGCCTCCTCATCTGCA
ACTCACCCCCATACTTGTTGCCTGCTGTTGAGAGCACCACGTACTCTGGATGCACAACTG
AGCATCTTGTGCAGCAGATTGGGGAGCGGNGGCACCACTTCTCCATTGNGTCTCCCCGCA
GCTGCCTGCGCTTTCGCTTCTGATTGAGAGGCAGCCCCCTCGCCTTGCTGAGCGCTGCAC
GCTCCGAAGATGTAGCTGGACCTGAGACAGGGGCTTGTTTGGGACTCGGCTGCTGTTGGG
GGGGTTTAACCTAGGCCCTTTAGTAAGGAGCCGGCCCTTGCTTCCCCGCACCTAGGGGCA
TTTTTTAAAGCA
>OriGene 3' read for NM_030973 unedited
TTTCCCTTGGGAATTAACTATGNACCCGCGGCCGCTTNCTANGATCGAGTTTTTTTTTTT
TTTTTTTATCATCAAATCGGATCTTTACTCAACTATGGTGAATGCATGGAACATAATACA
AACATGGATGTTACTGGAGTTTATATTTTTAAATTCAAAATTGAAAAAATACAAATTGAA
AAATAGAAATATTTAATTATTAAAATTTTCTCTTTCATAATTCTTTTTGCGCCTTTGAGT
TGAAGCCAAAAGGCTGGCGTCTTCCCTTCCACACCGAGATGTCAGTGTCAGGCCTCGGGT
GGCACGGTGGGGACGGGCAGTTTAGAATTCAGCCTTGAATCCTTCAACTGGGAACGATGT
TTAATTTGATTCAGGTTCGTAACGGAAAACAGCTGTTCACAAGCGTGGGTACTCCCAAAC
ACAGATATTCAGGACAAACTACTGGAGATAGTTGTAGAATCTCACCATCTTCACACAGAC
TTGTATTTAGGAACCAGCGCCGTATTGCACTGTAGTTCAATTACTATTTATGGTCTGCAT
TCACACAAGAAACATCTGCCAACAAAGGCAAACTGGACAGTGTCTGTTCCTTCTCATGAG
TCTGGATTCCGAGAGCCTTTGGGGGAATTTGGTCTTCAACCCCAGGACGTAGGTGAATAC
AGTCCGGGCCACCTCTTTCATTTCTGAAAGCCGACTGCAGCACAGGAAAGAGTAAAGGTT
TTTTTTCCTTGCCGGTGGATTCCCCAGGACCAAGCATACACCATCATACATTCAAGTAGC
TGTTTAGGGACATCCTTGCGGAGAAATATTCAGTGTTCAGAACGGTCATAACCTTGACCA
GAAGTCTTTGA
Restriction Sites NotI-NotI     
ACCN NM_030973
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030973.2, NP_112235.2
RefSeq Size 2335 bp
RefSeq ORF 2244 bp
Locus ID 81857
UniProt ID Q71SY5
Gene Summary This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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