NME2 (NM_002512) Human Untagged Clone
CAT#: SC108805
NME2 (untagged)-Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1
CNY 1,800.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_002512, the custom clone sequence may differ by one or more nucleotides
ATGGCCAACCTGGAGCGCACCTTCATCGCCATCAAGCCGGACGGCGTGCAGCGCGGCCTGGTGGGCGAGA TCATCAAGCGCTTCGAGCAGAAGGGATTCCGCCTCGTGGCCATGAAGTTCCTCCGGGCCTCTGAAGAACA CCTGAAGCAGCACTACATTGACCTGAAAGACCGACCATTCTTCCCTGGGCTGGTGAAGTACATGAACTCA GGGCCGGTTGTGGCCATGGTCTGGGAGGGGCTGAACGTGGTGAAGACAGGCCGAGTGATGCTTGGGGAGA CCAATCCAGCAGATTCAAAGCCAGGCACCATTCGTGGGGACTTCTGCATTCAGGTTGGCAGGAACATCAT TCATGGCAGTGATTCAGTAAAAAGTGCTGAAAAAGAAATCAGCCTATGGTTTAAGCCTGAAGAACTGGTT GACTACAAGTCTTGTGCTCATGACTGGGTCTATGAATAA >OriGene 5' read for NM_002512 unedited
GCACGAGGCCCGGGTGTGGTGGTCGCTCCAGCTCTCTGCTCTCCCAGCGCAGCGCCGCCG CCCGGCCCCTCCAGCTTCCCGGACCATGGCCAACCTGGAGCGCACCTTCATCGCCATCAA GCCGGACGGCGTGCAGCGCGGCCTGGTGGGCGAGATCATCAAGCGCTTCGAGCAGAAGGG ATTCCGCCTCGTGGCCATGAAGTTCCTCCGGGCCTCTGAAGAACACCTGAAGCAGCACTA CATTGACCTGAAAGACCGACCATTCTTCCCTGGGCTGGTGAAGTACATGAACTCAGGGCC GGTTGTGGCCATGGTCTGGGAGGGGCTGAACGTGGTGAAGACAGGCCGAGTGATGCTTGG GGAGACCAATCCAGCAGATTCAAAGCCAGGCACCATTCGTGGGGACTTCTGCATTCAGGT TGGCAGGAACATCATTCATGGCAGTGATTCAGTAAAAAGTGCTGAAAAAGAAATCAGCCT ATGGTTTAAGCCTGAAGAACTGGTTGACTACAAGTCTTGTGCTCATGACTGGGTCTATGA ATAAGAGGTGGACACAACAGCAGTCTCCTTCAGCACGGCGTGGTGTGTCCCTGGACACAG CTCTTCATTCCATTGACTTAGAGGCAACAGGATTGATCATTCTTTTATAGAGCATATTTG CCAATAAAGCTTTTGGGAAG >OriGene 3' read for NM_002512 unedited
GCTTGCAAGCTTCTGGTTTATTGCTCTATAAAGNATGATCAATCCTGTTGCCTCTAAGTC AATGGAATGAAGAGCTGTGTCCAGGGACACACCACGCCGTGCTGAAGGAGACTGCTGTTG TGTCCACCTCTTATTCATAGACCCAGTCATGAGCACAAGACTTGTAGTCAACCAGTTCTT CAGGCTTAAACCATAGGCTGATTTCTTTTTCAGCACTTTTTACTGAATCACTGCCATGAA TGATGTTCCTGCCAACCTGAATGCAGAAGTCCCCACGAATGGTGCCTGGCTTTGAATCTG CTGGATTGGTCTCCCCAAGCATCACTCGGCCTGTCTTCACCACGTTCAGCCCCTCCCAGA CCATGGCCACAACCGGCCCTGAGTTCATGTACTTCACCAGCCCAGGGAAGAATGGTCGGT CTTTCAGGTCAATGTAGTGCTGCTTCAGGTGTTCTTCAGAGGCCCGGAGGAACTTCATGG CCACGAGGCGGAATCCCTTCTGCTCGAAGCGCTTGATGATCTCGCCCACCAGGCCGCGCT GCACGCCGTCCGGCTTGATGGCGATGAAGGTGCGCTCCAGGTTGGCCATGGTCCGGGAAG CTGGAGGGGCCGGGCGGCGGCGCTGCGCTGGGAGAGCAGAGAGCTGGAGCGACCACCACA CCCGGGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGAC GGTTCACTAAACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGCCCATTT GCGTCAACGGGGCGGGGTTATTACGACATTTTGGGAAAGTCCCGTTGATTTTGGTGCCAA ACATACTCCCATGACGTCATGGGGTGGAGACTGGAAATCCCGGGAGTAAACCGTTNCACC CCATGTGTCTGCCAAACGCTCACATGTATAGCGAGACTATC |
Restriction Sites | NotI-NotI |
ACCN | NM_002512 |
Insert Size | 840 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002512.2, NP_002503.1 |
RefSeq Size | 822 bp |
RefSeq ORF | 459 bp |
Locus ID | 4831 |
UniProt ID | P22392 |
Domains | NDK |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
Gene Summary | Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010] Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1-4 encode the same isoform. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219564 | NME2 (Myc-DDK-tagged)-Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1 |
CNY 1,800.00 |
|
RC219564L1 | Lenti ORF clone of Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
RC219564L2 | Lenti ORF clone of Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC219564L3 | Lenti ORF clone of Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC219564L4 | Lenti ORF clone of Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG219564 | NME2 (tGFP-tagged) - Human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 1 |
CNY 3,400.00 |