GGT1 (NM_013421) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD224; D22S672; D22S732; GGT; GGT 1; GGTD; GTG
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_013421, the custom clone sequence may differ by one or more nucleotides ATGAAGAAGAAGTTAGTGGTGCTGGGCCTGCTGGCCGTGGTCCTGGTGCTGGTCATTGTCGGCCTCTGTC TCTGGCTGCCCTCAGCCTCCAAGGAACCTGACAACCATGTGTACACCAGGGCTGCCGTGGCCGCGGATGC CAAGCAGTGCTCGAAGATTGGGAGGGATGCACTGCGGGACGGTGGCTCTGCGGTGGATGCAGCCATTGCA GCCCTGTTGTGTGTGGGGCTCATGAATGCCCACAGCATGGGCATCGGGGGTGGCCTCTTCCTCACCATCT ACAACAGCACCACACGAAAAGCTGAGGTCATCAACGCCCGCGAGGTGGCCCCCAGGCTGGCCTTTGCCAC CATGTTCAACAGCTCGGAGCAGTCCCAGAAGGGGGGGCTGTCGGTGGCGGTGCCTGGGGAGATCCGAGGC TATGAGCTGGCACACCAGCGGCATGGGCGGCTGCCCTGGGCTCGCCTCTTCCAGCCCAGCATCCAGCTGG CCCGCCAGGGCTTCCCCGTGGGCAAGGGCTTGGCGGCAGCCCTGGAAAACAAGCGGACCGTCATCGAGCA GCAGCCTGTCTTGTGTGAGGTGTTCTGCCGGGATAGAAAGGTGCTTCGGGAGGGGGAGAGACTGACCCTG CCGCAGCTGGCTGACACCTACGAGACGCTGGCCATCGAGGGTGCCCAGGCCTTCTACAACGGCAGCCTCA CGGCCCAGATTGTGAAGGACATCCAGGCGGCCGGGGGCATTGTGACAGCTGAGGACCTGAACAACTACCG TGCTGAGCTGATCGAGCACCCGCTGAACATCAGCCTGGGAGACGCGGTGCTGTACATGCCCAGTGCGCCG CTCAGCGGGCCCGTGCTGGCCCTCATCCTCAACATCCTCAAAGGGTACAACTTCTCCCGGGAGAGCGTGG AGAGCCCCGAGCAGAAGGGCCTGACGTACCACCGCATCGTAGAGGCTTTCCGGTTTGCCTACGCCAAGAG GACCCTGCTTGGGGACCCCAAGTTTGTGGATGTGACTGAGGTGGTCCGCAACATGACCTCCGAGTTCTTC GCTGCCCAGCTCCGGGCCCAGATCTCTGACGACACCACTCACCCGATCTCCTACTACAAGCCCGAGTTCT ACACGCCGGATGACGGGGGCACTGCTCACCTGTCTGTCGTCGCAGAGGACGGCAGTGCTGTGTCCGCCAC CAGCACCATCAACCTCTACTTTGGCTCCAAGGTCCGCTCCCCGGTCAGCGGGATCCTGTTCAATAATGAA ATGGACGACTTCAGCTCTCCCAGCATCACCAACGAGTTTGGGGTACCCCCCTCACCTGCCAATTTCATCC AGCCAGGGAAGCAGCCGCTCTCGTCCATGTGCCCGACGATCATGGTGGGCCAGGACGGCCAGGTCCGGAT GGTGGTGGGAGCTGCTGGGGGCACACAGATCACCACGGCCACTGCACTGGCCATCATCTACAACCTCTGG TTCGGCTATGACGTGAAGCGGGCCGTGGAGGAGCCCCGGCTGCACAACCAGCTTCTGCCCAACGTCACGA CAGTGGAGAGAAACATTGACCAGGCAGTGACTGCAGCCCTGGAGACCCGGCACCATCACACCCAGATCGC GTCCACCTTCATCGCTGTGGTGCAAGCCATCGTCCGCACGGCTGGTGGCTGGGCAGCTGCCTCGGACTCC AGGAAAGGCGGGGAGCCTGCCGGCTACTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_013421 unedited AGAAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCCGGTCAG AGCTTTAAGCTGCAGCTCTGATGCCCCAGGGACCTCTCAGACCCCCCTGCCCAGAGCAGG CAGCCCGCGGGTGCAGCCCAGAACTGTCTTCTGAGGAAGAGGTGCTCTCCTGGGCCCCCA CTGTCCCCAGGCCTCAGCAGCAAGGCAAGTGAGGTGCTGCCGTCATCCAGGCTGGACAGT TCAGTGATTTGCCTGAGGCCCCACAGCAGAGTTCAACTGGAGACAGAGAAACCAGCTAGA GGCAGAGGGAGGTAACACGGAGTCCCCCAGAAAGGTCTGGGCTGCGCGTGCTTCAGGTAA CCTCCCTTGACCTTCAGGAGAACGAGAAGGCTGTCTGATCAGAGAGTCCCTGAAGAAGAT TCTGTGGCTACAGGCTTCAGCAGAGTGTGAGGGAGACCCCGGTTATTTCCTCAGCTATTT CCACCAAATCCTCCTGTCTTTCGTGGCCAACACCCCAGGCAAGGCTTGGGGCCCCCGTCT GCTGCTGGACGCAGAGCCATGAAGAAGAAGTTAGTGGTGCTGGGCCTGCTGGCCGTGGTC CTGGTGCTGGTCATTGTCGGCCTCTGTCTCTGGCTGCCCTCAGCCTCCAAGGAACCTGAC AACCATGTGTACACCAGGGCTGCCGTGGCCGCGGATGCCAAGCAGTGCTCGAAGATTGGG AGGGATGCACTGCGGGACGGTGGCTCTGCGGTGGATGCAGCCATTGCAGCCCTGTTGTGT GTGGGGCTCATGAATGCCCACAGCATGGGCATCGGGGGGTGGCCTCTTCCTCACATCTAC AACAGCACCACACGAAAAAGCTGAGTCAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_013421 unedited GCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG AGGGGGGAAACAGGCCAAGGGAGGCCACCTGGAGCCTGGCACAGTGGCCTCATTTATTGG GCTGCTCTGCTGCTCACAAGGGAAACCGGTCCCCCAAAGTCCCCTTCCTGGGCCTGGGGA GTATTTTGTCCCTGGATTGCTTGTCAACCTTGTCCTCCTGGAGCACTCATTAGCCGGCAG GCTCCCCGCCTTTTCTGGAGTCCGAGGGAGCTGCCCAACCACCAACCGTGCGGACGATGG CTTGCACCACAGCGATGAAAGGGGACCCCATCTGGGTGTGATGGGGCCGGGTCTCCAAGG CTGGAGTCACTGCCTGGTCAATGGTTCTCTCCACTGTCGTGACGTTGGGCAAAAACTGGT TGTGCAACCGGGGCTTCTCCACGGGCCGCTTTACGTTATATCCGAACCAAAAGTTGTAAA TGATGGCCAATGCAGTGGCCGTGGTGATCTGTGTGCCCCCAGCAGCTCCCACCACCATTC GGACCTGGCCGTTCTGGCCCACCATGATCGTTGGGCACATGGACCAGAGCGGCTGCTTCC CTGGCTGGATGAAATTGGGCAGTGAAGGGGGTACCCCAAACTTCGTTGGAGATAGCTGGG AAAACCTAAATTCTCCATTTCATTATTGACAAGATCCCGCTGACCGGGAGCGGACCTTGG AGCCAAATANAAGTTGATGGGGCCTGTGGCGGACACAAGACTGTCGCTCTTTGGGACGAA AGAAAGGTGAACATTGCCCCCTTCTTCGGCGTGTAAAAACTCGGCTTTGATAAGGAGATC GGCTGAATGGTGTTCCCAAAAATTCTGGCCCCGGAGCTGGCCNCCAATAATTCGGAGTCA TGTTGCGGACACACTCATCCATTCACAAATTGGGGGTCCCAAGAGGGTCCTTTTGAGGTT GGAAACCGAAACACCTTACATGGNGGGTCCTAAGGCCCTTTGTTGGGGTTTC
Restriction Sites	NotI-NotI
ACCN	NM_013421
Insert Size	2590 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013421.1, NP_038265.1
RefSeq Size	2621 bp
RefSeq ORF	1710 bp
Locus ID	2678
UniProt ID	P19440
Domains	G_glu_transpept
Protein Families	Protease, Transmembrane
Protein Pathways	Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
Gene Summary	The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 6. Variants 2, 3, and 6 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.