DHFRL1 (DHFR2) (NM_176815) Human Untagged Clone
CAT#: SC108348
DHFRL1 (untagged)-Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DHFRL1; DHFRP4 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC108348 sequence for NM_176815 edited (data generated by NextGen Sequencing)
ATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACATGGGCATCGGCAAGAAC GGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATTTCCAGAGAATGACCACA ACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTAGGAAGACCTGGTTCTCC ATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAGTTCTCAGCAGAGAACTC AAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGGATGATGCCTTAAAACTT ACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGATAGTTGGTGGCAGTTCT GTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTATTTGTGACAAGGATCATG CAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGGAGAAATATAAACTTCTG CCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAACACATCAAGTACAAATTT GAAGTATGTGAGAAGGATGATTAA Clone variation with respect to NM_176815.4 >OriGene 5' read for NM_176815 unedited
NGGGGGTTCAGCAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACA CATCTGCGCGCCTGTGCGGCCCCTGCTTCTGCGGATGCTGAGGCACGTAAAAAAATTTGA AGAAGGGAATTTCGCGGCATTCTTGGCCTGGCTTCCTGGCGTAGCCAGCAAGTTCGGAGG TGTTAACCGCTGCTGTCATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACA TGGGCATCGGCAAGAACGGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATT TCCAGAGAATGACCACAACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTA GGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAG TTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGG ATGATGCCTTAAAACTTACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGA TAGTTGGTGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTAT TTGTGACAAGGATCATGCAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGG AGAAATATAAACTTCTGCCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAAC ACATCAAGTACAAATTTGAAGTATGTGAGAAGGATGATTAATATGAAANGGTGTTTCTGG TTTAAGNTGTTCCCCCTCCCTCTGAGAAAAGTATGCATTTTTACATTAGAAAAAGGACTT TTGTTGACTTCAGATCTATGGNATAATTATTTCTAAGCACGTGTTTTTATTCCTCACTAA TCTGGCCTTNTCCAGATCCAT >OriGene 3' read for NM_176815 unedited
NNGGGTCCTGNCATGNACCGCGGCACGCAATCTAAGATCGAGTTTTTTTTTTTTTTTTTT ACTTTTGCTTTATCTTCAATGCACTTCTTTCCAAATGCCCCAAATCTGGCAATAACCTAT TATTTTTAAGAGAAGGGATCTCACTATGGTGGCCAGGCTGCTCTGAACTCTTGGGCTCAA GCCATCCTTTTGCTTAGCCTCCTGAGTAGCTGGGACTACAAGCATGGGCCATCCCACCCA GCTAATCTATTAGTATTTTTACTGTTTCTTTTTAAAGCTAGCTCCATAGCTGGAATATTG CCTGTGTTGGATACTATTTGCTGGATCCACGTCATTCTCTGGTTTGTGTACGCCTTCATT TTGCTGGAGTATTTTCTCCGATAGCTTCCCAAGAAAGGGTACATGTAACCCCTGAGTCTT TGCTTGTCTAAAAATGTATTATTTTACCTTCACCCTTGATTCTTTGCCTGAATATAGATT TATCAGTTGAAAATAACTTTATAGAATTCTGAAGGCATGGTTCCGTTGTTTTCAGATTCT TTTTTGAGACAGGGTCTCTTCTGTCACCCAGGCAGGAGTGCAGTGGCAAAATCACGACTC ACTGCAGCCTTGAACTCATGGGCTCAATTGAATCCTCCATCTCAGCCCCGTGAGTAGACG AGACTACAGGTGCGTGCCACAATGCCCAGCCAATTTTTGTATCTTCTGTAGAGACAGGGC TCCACCATGTTGCCCAAGCTGGTCTCAAGTGATCCACCCACCTCATCCCCCCAAATGCTG GGACCCACAGCGTGAGCCACCCCTGCTGGCCAGTTTTAAATTCCCTCCCTAACTCTTTCA TTTCTCAAAGGGCTCTCAACCTCGTGGGGCCCAATAC |
Restriction Sites | NotI-NotI |
ACCN | NM_176815 |
Insert Size | 3300 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_176815.2, NP_789785.1 |
RefSeq Size | 3772 bp |
RefSeq ORF | 564 bp |
Locus ID | 200895 |
UniProt ID | Q86XF0 |
Protein Families | Druggable Genome |
Gene Summary | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208452 | DHFRL1 (Myc-DDK-tagged)-Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2 |
CNY 2,400.00 |
|
RC208452L3 | Lenti ORF clone of Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208452L4 | Lenti ORF clone of Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG208452 | DHFRL1 (tGFP-tagged) - Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2 |
CNY 4,000.00 |