DHFRL1 (DHFR2) (NM_176815) Human Untagged Clone

CAT#: SC108348

DHFRL1 (untagged)-Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2



  "NM_176815" in other vectors (4)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
DHFRL1 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DHFRL1; DHFRP4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC108348 sequence for NM_176815 edited (data generated by NextGen Sequencing)
ATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACATGGGCATCGGCAAGAAC
GGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATTTCCAGAGAATGACCACA
ACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTAGGAAGACCTGGTTCTCC
ATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAGTTCTCAGCAGAGAACTC
AAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGGATGATGCCTTAAAACTT
ACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGATAGTTGGTGGCAGTTCT
GTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTATTTGTGACAAGGATCATG
CAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGGAGAAATATAAACTTCTG
CCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAACACATCAAGTACAAATTT
GAAGTATGTGAGAAGGATGATTAA

Clone variation with respect to NM_176815.4
>OriGene 5' read for NM_176815 unedited
NGGGGGTTCAGCAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACA
CATCTGCGCGCCTGTGCGGCCCCTGCTTCTGCGGATGCTGAGGCACGTAAAAAAATTTGA
AGAAGGGAATTTCGCGGCATTCTTGGCCTGGCTTCCTGGCGTAGCCAGCAAGTTCGGAGG
TGTTAACCGCTGCTGTCATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACA
TGGGCATCGGCAAGAACGGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATT
TCCAGAGAATGACCACAACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTA
GGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAG
TTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGG
ATGATGCCTTAAAACTTACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGA
TAGTTGGTGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTAT
TTGTGACAAGGATCATGCAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGG
AGAAATATAAACTTCTGCCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAAC
ACATCAAGTACAAATTTGAAGTATGTGAGAAGGATGATTAATATGAAANGGTGTTTCTGG
TTTAAGNTGTTCCCCCTCCCTCTGAGAAAAGTATGCATTTTTACATTAGAAAAAGGACTT
TTGTTGACTTCAGATCTATGGNATAATTATTTCTAAGCACGTGTTTTTATTCCTCACTAA
TCTGGCCTTNTCCAGATCCAT
>OriGene 3' read for NM_176815 unedited
NNGGGTCCTGNCATGNACCGCGGCACGCAATCTAAGATCGAGTTTTTTTTTTTTTTTTTT
ACTTTTGCTTTATCTTCAATGCACTTCTTTCCAAATGCCCCAAATCTGGCAATAACCTAT
TATTTTTAAGAGAAGGGATCTCACTATGGTGGCCAGGCTGCTCTGAACTCTTGGGCTCAA
GCCATCCTTTTGCTTAGCCTCCTGAGTAGCTGGGACTACAAGCATGGGCCATCCCACCCA
GCTAATCTATTAGTATTTTTACTGTTTCTTTTTAAAGCTAGCTCCATAGCTGGAATATTG
CCTGTGTTGGATACTATTTGCTGGATCCACGTCATTCTCTGGTTTGTGTACGCCTTCATT
TTGCTGGAGTATTTTCTCCGATAGCTTCCCAAGAAAGGGTACATGTAACCCCTGAGTCTT
TGCTTGTCTAAAAATGTATTATTTTACCTTCACCCTTGATTCTTTGCCTGAATATAGATT
TATCAGTTGAAAATAACTTTATAGAATTCTGAAGGCATGGTTCCGTTGTTTTCAGATTCT
TTTTTGAGACAGGGTCTCTTCTGTCACCCAGGCAGGAGTGCAGTGGCAAAATCACGACTC
ACTGCAGCCTTGAACTCATGGGCTCAATTGAATCCTCCATCTCAGCCCCGTGAGTAGACG
AGACTACAGGTGCGTGCCACAATGCCCAGCCAATTTTTGTATCTTCTGTAGAGACAGGGC
TCCACCATGTTGCCCAAGCTGGTCTCAAGTGATCCACCCACCTCATCCCCCCAAATGCTG
GGACCCACAGCGTGAGCCACCCCTGCTGGCCAGTTTTAAATTCCCTCCCTAACTCTTTCA
TTTCTCAAAGGGCTCTCAACCTCGTGGGGCCCAATAC
Restriction Sites NotI-NotI     
ACCN NM_176815
Insert Size 3300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_176815.2, NP_789785.1
RefSeq Size 3772 bp
RefSeq ORF 564 bp
Locus ID 200895
UniProt ID Q86XF0
Protein Families Druggable Genome
Gene Summary Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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