EIF2B4 (NM_015636) Human Untagged Clone

CAT#: SC108336

EIF2B4 (untagged)-Human eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 3



  "NM_015636" in other vectors (4)

CNY 7,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
EIF2B4 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EIF-2B; EIF2B; EIF2Bdelta
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_015636, the custom clone sequence may differ by one or more nucleotides


ATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTCGGGATCCGGGATGAAGGCGGAGCTTCCCCCTGGGC
CTGGGGCAGTGGGGAGGGAAATGACCAAAGAAGAAAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAA
GAAGAAACGGAAGGAAGAAAAGGGGGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAA
GGCCCAACCAGAGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTC
GGAGTAAGGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGAAACAGGCAAG
AAAAGGGGAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTGGAGAAACCCCCTCAGGAGTG
AAGCGTCTCCCTGAGTACCCTCAGGTTGATGACCTACTTCTGAGAAGGCTTGTTAAAAAACCAGAGCGTC
AACAGGTTCCTACACGAAAGGATTATGGATCCAAAGTCAGTCTCTTCTCTCACCTACCCCAGTACAGCAG
ACAAAACTCTCTGACCCAGTTTATGAGCATCCCATCCTCTGTGATCCACCCAGCCATGGTGCGACTCGGC
CTGCAGTACTCCCAGGGCCTGGTCAGTGGCTCCAATGCCCGGTGTATTGCCCTGCTTCGTGCCTTGCAGC
AGGTGATTCAGGATTACACAACACCGCCTAATGAAGAACTCTCCAGGGATCTAGTGAATAAACTAAAACC
CTACATGAGCTTCCTGACTCAGTGCCGTCCCCTGTCAGCGAGCATGCACAACGCCATCAAGTTCCTTAAC
AAGGAAATCACCAGTGTGGGCAGTTCCAAGCGGGAAGAGGAGGCCAAGTCAGAACTTCGAGCAGCCATTG
ATCGGTATGTGCAAGAGAAGATTGTGCTAGCAGCTCAGGCAATTTCACGCTTTGCTTACCAGAAGATCAG
TAATGGAGATGTGATCCTGGTATATGGATGCTCATCTCTGGTATCACGAATTCTTCAGGAGGCTTGGACA
GAGGGCCGGCGGTTTCGGGTGGTAGTGGTGGACAGCCGGCCATGGCTGGAAGGAAGGCACACACTACGTT
CTCTAGTCCATGCTGGTGTCCCAGCCTCCTACCTGCTGATTCCTGCAGCCTCCTATGTGCTCCCAGAGGT
TTCCAAGGTGCTATTGGGAGCTCATGCACTCTTGGCCAACGGGTCTGTGATGTCACGGGTAGGGACAGCA
CAGTTAGCCCTGGTGGCTCGAGCCCATAATGTACCAGTGCTGGTTTGCTGTGAAACATACAAGTTCTGTG
AGCGTGTGCAGACTGATGCCTTTGTCTCTAATGAGCTAGATGACCCTGATGATCTGCAATGTAAGCGGGG
AGAACATGTTGCGCTGGCTAACTGGCAGAACCACGCATCCCTACGGTTGTTGAATCTAGTCTATGATGTG
ACTCCCCCAGAGCTTGTGGATCTGGTGATCACGGAGCTGGGGATGATCCCTTGCAGTTCTGTACCTGTTG
TTCTACGAGTCAAGAGCAGTGACCAGTGA


>OriGene 5' read for NM_015636 unedited
ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGGGGCGGCTCAGCCTCCACCCGGTGG
CGCTGTGGACCCTGCAGCCGCGGAGCGAGGCAGCGGGGCGGCTCCGTGACCACGGCGGGC
GGAGCCTAGGACTGAGGGCGATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTCGGGAT
CCGGGATGAAGGCGGAGCTTCCCCCTGGGCCTGGGGCAGTGGGGAGGGAAATGACCAAAG
AAGAAAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAAGAAGAAACGGAAGGAAGAAA
AGGGGGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAAGGCCCAACCA
GAGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTC
GGAGTAAGGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGA
AACAGGCAAGAAAAGGGGAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTG
GAGAAACCCCCTCAGGAGTGAAGCGTCTCCCTGAGTACCCTCAGGTTGATGACCTACTTC
TGAGAAGGCTTGTTAAAAAACCAGAGCGTCAACAGGTAGGAAGTGGGTTTGGTGCCTGGG
CTAGAAGAGCCTANGGAAATGGACTGGANGGAGGAAAGGTGCNTGGTGCANAGTGAGAAG
GATAGGTGCTTGGGTCANAGTAGTCTTACTGGTCACTTCCTTTCAGCNTTATCCCATCCT
TTNTAAACTCTNGGCCATNGNTCCTGTNCCCTTGNNTTGTCAGGNTCCTACACGAAGGAT
TATGGATCCAAGTCAGTCTCTCTCTCACTACCCNAGTCAGCAGACAAACTCTCTGACCAG
TNATGAGGTAGATCCTATGAANTGNCATACTTTGAGTCTGAGAGTTTANTAGTCGTATGG
AAGTCTATATCTGAATGACTTG
>OriGene 3' read for NM_015636 unedited
ACCTCAGAGNACCAGGCCGCTTTCTAAGATCGGTTTTTTTTTTTTTTTTTTGCTGAGGGT
AGGGAGTATGGCATTTATTAACCCTGTGTTTCCCCCGTCACTGGTCACTGCTCTTGACTC
GTAGAACAACAGGTACAGAACTGCAAGGGATCATCCCCAGCTCCGTGATCACCAGATCCA
CAAGCTCTGGGGGAGTCACATCATAGACTAGATTCAACAACCGTAGGGATGCGTGGTTCT
GCCAGTTAGCCAGCGCAACATGTTCTCCCCGCTTACATTGCAGATCATCAGGGTCATCTA
GCTCATTAGAGACAAAGGCATCAGTCTGCACACGCTCACAGAACTTGTATGTTTCACAGC
AAACCAGCACTGGTACATTATGGGCTCGAGCCACCAGGGCTAACTGTGCTGTCCCTACCC
GTGACATCACAGACCCGTTGGCCAAGAGTGCATGAGCTCCCAATAGCACCTTGGAAACCT
CTGGGAGCACATAGGAGGCTGCAGGAATCAGCAGGTAGGAGGCTGGGACACCAGCATGGA
CTAGAGAACGTAGTGTGTGCCTTCCTTCCAGCCATGGCCGGCTGTCCACCACTACCACCC
GAAACCGCCGGCCCTCTGTCCAAGCCCCCTGAAGAATTCGTGATACCAGAGATGAGCATC
CATATACCAGGATCACATCTCCATTACTGATCTTCTGGTAAGCAAAGCGTGAAATTGCCT
GAGCTGCTAGCACAATCTTTCTCTTGCCCATACCGATCAATGGCTGCTCGAAGTTCTGAC
CTGGCCTAAATGGAGTAAAATCCCTAACCGAACAAGGAAATGGACACCTTTATACCCTCT
GATGATCATGGCCAGGGGCCTGAATTTGCCGTATGATGACAAGCCCATAGAAGGCTCANA
GAAGGCCACAGTCATAATGGCCAACCATTCCTTTACCCCTGCATAGGGCAACAAAC
Restriction Sites NotI-NotI     
ACCN NM_015636
Insert Size 2320 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015636.2, NP_056451.2
RefSeq Size 1643 bp
RefSeq ORF 1569 bp
Locus ID 8890
UniProt ID Q9UI10
Domains IF-2B
Gene Summary Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction compared to variant 4. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an internal aa compared to isoform 4.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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