NIT2 (NM_020202) Human Untagged Clone
CAT#: SC108295
NIT2 (untagged)-Human nitrilase family, member 2 (NIT2)
CNY 2,400.00
CNY 6,270.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HEL-S-8a |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC108295 sequence for NM_020202 edited (data generated by NextGen Sequencing)
ATGACCTCTTTCCGCTTGGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAAC GTCACTCGCGCTTGTAGCTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCT TTGCCGGAATGCTTTAATTCTCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAA ATTCCTGGTGAATCCACACAGAAGCTTTCTGAAGTAGCAAAGGAATGCAGCATATATCTC ATTGGAGGCTCTATCCCTGAAGAGGATGCTGGGAAATTATATAACACCTGTGCTGTGTTT GGGCCTGATGGAACTTTACTAGCAAAGTATAGAAAGATCCATCTGTTTGACATTGATGTT CCTGGAAAAATTACATTTCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACA TTTGATACTCCTTACTGCAGAGTGGGTCTGGGCATCTGCTACGACATGCGGTTTGCAGAG CTTGCACAAATCTACGCACAGAGAGGCTGCCAGCTGTTGGTATATCCAGGAGCTTTTAAT CTGACCACTGGACCAGCCCATTGGGAGTTACTTCAGCGAAGCCGGGCTGTTGATAATCAG GTGTATGTGGCCACAGCCTCTCCTGCCCGGGATGACAAAGCCTCCTATGTTGCCTGGGGA CACAGCACCGTGGTGAACCCTTGGGGGGAGGTTCTAGCCAAAGCTGGCACAGAAGAAGCA ATCGTGTATTCAGACATAGACCTGAAGAAGCTGGCTGAAATACGCCAGCAAATCCCCGTT TTTAGACAGAAGCGATCAGACCTCTATGCTGTGGAGATGAAAAAGCCCTAA Clone variation with respect to NM_020202.4 >OriGene 5' read for NM_020202 unedited
NGGTCGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATCGGCACGAGGCCGGATCTC CAGCGCTCAGTCCGCGCCGCAGGTGGTGCTTGTCTGCAGAGTCATGACCTCTTTCCGCTT GGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAACGTCACTCGCGCTTGTAG CTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCTTTGCCGGAATGCTTTAA TTCTCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAAATTCCTGGTGAATCCAC ACAGAAGCTTTCTGAAGTAGCAAAGGAATGCAGCATATATCTCATTGGAGGCTCTATCCC TGAAGAGGATGCTGGGAAATTATATAACACCTGTGCTGTGTTTGGGCCTGATGGAACTTT ACTAGCAAAGTATAGAAAGATCCATCTGTTTGACATTGATGTTCCTGGAAAAATTACATT TCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACATTTGATACTCGTATGTA CCAGATAAGTTTGCCTCTTTAGCAATCTCAGTAGAAGACAATCAGGTATTTATTTCTTTT TTGTCTCTCTCCGATTTCTTCACATAACCTAACTGAAAGACCATAAGTGAGAAAGGCAGA GAATCATCACAGATCTGGAAAGTTCGGGCTTATTTGAGAACTAANGATTTGACACGATTT TGCCCTTTGATTTGATTGTAGCTTCCTGTTATGGCTTCCAGAGTATACCTATTAGGCTAC CGTTGAGTACCTCCCATCTAGATCATAAGCATTCAATTAGAAATGAATTTCTCATCCTTA GTCCGCTGAAGTAAATGCAGTCTTTATGAAATGAAGCCCAGTAAGAATGAGCTCGTAATT A >OriGene 3' read for NM_020202 unedited
CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCATTAAAGAATTTAATAGGGA GTTGATTATGTTGTAGAATCATATCGTCCTATTCTGTGACACATTAAAAACATAAACTTT AGGGCTTTTTCATCTCCACAGCATAGAGGTCTGATCGCTTCTGTCTAAAAACGGGGATTT GCTGGCGTATTTCAGCCAGCTTCTTCAGGTCTATGTCTGAATACACGATTGCTTCTTCTG TGCCAGCTTTGGCTAGAACCTCCCCCCAAGGGTTCACCACGGTGCTGTGTCCCCAGGCAA CATAGGAGGCTTTGTCATCCCGGGCAGGAGAGGCTGTGGCCACATACACCTGATTATCAA CAGCCCGGCTTCGCTGAAGTAACTCCCAATGGGCTGGTCCAGTGGTCAGATTAAAAGCTC CTGGATATACCAACAGCTGGCAGCCTCTCTGTGCGTAGATTTGTGCAAGCTCTGCAAACC GCATGTCGTAGCAGATGCCCAGACCCACTCTGCAGTAAGCTGTTTCCAAACAGAAAATGC ACAAATGAAAAGTTAACTCCCTACAGCATGGTGTGGCAGGGGAAATCCTCCATGACATAA TGATCACAAAACACTGACAGGGATAAATTAACACAGGACCTAAGGACAGAGATAATTTAC AAGCTCATTCCTACTTGGACTTCATCTCATAAAGACATCATTTACATCAGCGGACTAAAG ATGAGAAATTCATTCTAANTGAATGCTTATTATCTAGATGGGAGGTACTCAACTGTAGCC TAATAGGTATACTCTGGAAGCCATACAGGGAGCTACAATCAAATCAAGGGCANAATCGTG TCAAATCCTTAGTTCTCAAATAGCCCGAACTTCCAGACCTGGGATGATTCTCTGCCTTTC TCACTTAGGGNCTTTCACTTAGGGTTATGGGAAGAAATCGGAGAGAGACAAAAAGAAAAA AACCCCTGATGGCCTCTACTTGAAATGCCTAAGAAGA |
Restriction Sites | NotI-NotI |
ACCN | NM_020202 |
Insert Size | 1500 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_020202.2, NP_064587.1 |
RefSeq Size | 1019 bp |
RefSeq ORF | 831 bp |
Locus ID | 56954 |
UniProt ID | Q9NQR4 |
Domains | CN_hydrolase |
Gene Summary | Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210660 | NIT2 (Myc-DDK-tagged)-Human nitrilase family, member 2 (NIT2) |
CNY 2,400.00 |
|
RC210660L1 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC210660L2 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), mGFP tagged |
CNY 5,890.00 |
|
RC210660L3 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210660L4 | Lenti ORF clone of Human nitrilase family, member 2 (NIT2), mGFP tagged |
CNY 5,890.00 |
|
RG210660 | NIT2 (tGFP-tagged) - Human nitrilase family, member 2 (NIT2) |
CNY 4,000.00 |