NIT2 (NM_020202) Human Untagged Clone

CAT#: SC108295

NIT2 (untagged)-Human nitrilase family, member 2 (NIT2)



  "NM_020202" in other vectors (6)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-NIT2 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-8a
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC108295 sequence for NM_020202 edited (data generated by NextGen Sequencing)
ATGACCTCTTTCCGCTTGGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAAC
GTCACTCGCGCTTGTAGCTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCT
TTGCCGGAATGCTTTAATTCTCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAA
ATTCCTGGTGAATCCACACAGAAGCTTTCTGAAGTAGCAAAGGAATGCAGCATATATCTC
ATTGGAGGCTCTATCCCTGAAGAGGATGCTGGGAAATTATATAACACCTGTGCTGTGTTT
GGGCCTGATGGAACTTTACTAGCAAAGTATAGAAAGATCCATCTGTTTGACATTGATGTT
CCTGGAAAAATTACATTTCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACA
TTTGATACTCCTTACTGCAGAGTGGGTCTGGGCATCTGCTACGACATGCGGTTTGCAGAG
CTTGCACAAATCTACGCACAGAGAGGCTGCCAGCTGTTGGTATATCCAGGAGCTTTTAAT
CTGACCACTGGACCAGCCCATTGGGAGTTACTTCAGCGAAGCCGGGCTGTTGATAATCAG
GTGTATGTGGCCACAGCCTCTCCTGCCCGGGATGACAAAGCCTCCTATGTTGCCTGGGGA
CACAGCACCGTGGTGAACCCTTGGGGGGAGGTTCTAGCCAAAGCTGGCACAGAAGAAGCA
ATCGTGTATTCAGACATAGACCTGAAGAAGCTGGCTGAAATACGCCAGCAAATCCCCGTT
TTTAGACAGAAGCGATCAGACCTCTATGCTGTGGAGATGAAAAAGCCCTAA

Clone variation with respect to NM_020202.4
>OriGene 5' read for NM_020202 unedited
NGGTCGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATCGGCACGAGGCCGGATCTC
CAGCGCTCAGTCCGCGCCGCAGGTGGTGCTTGTCTGCAGAGTCATGACCTCTTTCCGCTT
GGCCCTCATCCAGCTTCAGATTTCTTCCATCAAATCAGATAACGTCACTCGCGCTTGTAG
CTTCATCCGGGAGGCAGCAACGCAAGGAGCCAAAATAGTTTCTTTGCCGGAATGCTTTAA
TTCTCCATATGGAGCGAAATATTTTCCTGAATATGCAGAGAAAATTCCTGGTGAATCCAC
ACAGAAGCTTTCTGAAGTAGCAAAGGAATGCAGCATATATCTCATTGGAGGCTCTATCCC
TGAAGAGGATGCTGGGAAATTATATAACACCTGTGCTGTGTTTGGGCCTGATGGAACTTT
ACTAGCAAAGTATAGAAAGATCCATCTGTTTGACATTGATGTTCCTGGAAAAATTACATT
TCAAGAATCTAAAACATTGAGTCCGGGTGATAGTTTCTCCACATTTGATACTCGTATGTA
CCAGATAAGTTTGCCTCTTTAGCAATCTCAGTAGAAGACAATCAGGTATTTATTTCTTTT
TTGTCTCTCTCCGATTTCTTCACATAACCTAACTGAAAGACCATAAGTGAGAAAGGCAGA
GAATCATCACAGATCTGGAAAGTTCGGGCTTATTTGAGAACTAANGATTTGACACGATTT
TGCCCTTTGATTTGATTGTAGCTTCCTGTTATGGCTTCCAGAGTATACCTATTAGGCTAC
CGTTGAGTACCTCCCATCTAGATCATAAGCATTCAATTAGAAATGAATTTCTCATCCTTA
GTCCGCTGAAGTAAATGCAGTCTTTATGAAATGAAGCCCAGTAAGAATGAGCTCGTAATT
A
>OriGene 3' read for NM_020202 unedited
CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCATTAAAGAATTTAATAGGGA
GTTGATTATGTTGTAGAATCATATCGTCCTATTCTGTGACACATTAAAAACATAAACTTT
AGGGCTTTTTCATCTCCACAGCATAGAGGTCTGATCGCTTCTGTCTAAAAACGGGGATTT
GCTGGCGTATTTCAGCCAGCTTCTTCAGGTCTATGTCTGAATACACGATTGCTTCTTCTG
TGCCAGCTTTGGCTAGAACCTCCCCCCAAGGGTTCACCACGGTGCTGTGTCCCCAGGCAA
CATAGGAGGCTTTGTCATCCCGGGCAGGAGAGGCTGTGGCCACATACACCTGATTATCAA
CAGCCCGGCTTCGCTGAAGTAACTCCCAATGGGCTGGTCCAGTGGTCAGATTAAAAGCTC
CTGGATATACCAACAGCTGGCAGCCTCTCTGTGCGTAGATTTGTGCAAGCTCTGCAAACC
GCATGTCGTAGCAGATGCCCAGACCCACTCTGCAGTAAGCTGTTTCCAAACAGAAAATGC
ACAAATGAAAAGTTAACTCCCTACAGCATGGTGTGGCAGGGGAAATCCTCCATGACATAA
TGATCACAAAACACTGACAGGGATAAATTAACACAGGACCTAAGGACAGAGATAATTTAC
AAGCTCATTCCTACTTGGACTTCATCTCATAAAGACATCATTTACATCAGCGGACTAAAG
ATGAGAAATTCATTCTAANTGAATGCTTATTATCTAGATGGGAGGTACTCAACTGTAGCC
TAATAGGTATACTCTGGAAGCCATACAGGGAGCTACAATCAAATCAAGGGCANAATCGTG
TCAAATCCTTAGTTCTCAAATAGCCCGAACTTCCAGACCTGGGATGATTCTCTGCCTTTC
TCACTTAGGGNCTTTCACTTAGGGTTATGGGAAGAAATCGGAGAGAGACAAAAAGAAAAA
AACCCCTGATGGCCTCTACTTGAAATGCCTAAGAAGA
Restriction Sites NotI-NotI     
ACCN NM_020202
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020202.2, NP_064587.1
RefSeq Size 1019 bp
RefSeq ORF 831 bp
Locus ID 56954
UniProt ID Q9NQR4
Domains CN_hydrolase
Gene Summary Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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