EMC9 (NM_016049) Human Untagged Clone
CAT#: SC108275
EMC9 (untagged)-Human family with sequence similarity 158, member A (FAM158A)
CNY 2,400.00
CNY 2,950.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | C14orf122; CGI-112; FAM158A |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC108275 sequence for NM_016049 edited (data generated by NextGen Sequencing)
ATGGGGGAGGTGGAGATCTCGGCCCTGGCCTACGTGAAGATGTGCCTGCATGCTGCCCGG TACCCACACGCCGCAGTCAACGGGCTGTTTTTGGCGCCAGCGCCGCGGTCTGGAGAATGC CTGTGCCTCACCGACTGTGTGCCCCTCTTCCACAGCCACCTGGCCCTGTCCGTCATGTTG GAGGTCGCCCTCAACCAGGTGGATGTGTGGGGAGCACAGGCCGGTCTGGTGGTGGCTGGT TACTACCATGCCAATGCAGCTGTGAACGATCAGAGCCCTGGGCCCCTGGCCTTGAAAATT GCTGGGCGAATTGCAGAATTCTTCCCTGATGCAGTACTTATTATGTTGGATAATCAGAAA CTGGTGCCTCAGCCTCGTGTGCCCCCGGTCATCGTCCTGGAGAACCAAGGTCTCCGCTGG GTCCCTAAGGATAAGAACTTAGTGATGTGGAGGGACTGGGAAGAGTCACGGCAGATGGTG GGAGCTCTACTGGAAGATCGGGCCCACCAGCACCTTGTGGACTTTGACTGCCACCTTGAT GACATCCGGCAGGACTGGACCAACCAGCGGCTCAACACTCAAATCACCCAGTGGGTTGGT CCCACTAATGGAAATGGAAATGCCTGA Clone variation with respect to NM_016049.3 >OriGene 5' read for NM_016049 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGAGCCGGCGGGCTACC TGCGAGTCGAGTTAGCGTTGTCGCCGAACCGAAGCCTCGCTCGCCATGGGGGAGGTGGAG ATCTCGGCCCTGGCCTACGTGAAGATGTGCCTGCATGCTGCCCGGTACCCACACGCCGCA GTCAACGGGCTGTTTTTGGCGCCAGCGCCGCGGTCTGGAGAATGCCTGTGCCTCACCGAC TGTGTGCCCCTCTTCCACAGCCACCTGGCCCTGTCCGTCATGTTGGAGGTCGCCCTCAAC CAGGTGGATGTGTGGGGAGCACAGGCCGGTCTGGTGGTGGCTGGTTACTACCATGCCAAT GCAGCTGTGAACGATCAGAGCCCTGGGCCCCTGGCCTTGAAAATTGCTGGGCGAATTGCA GAATTCTTCCCTGATGCAGTACTTATTATGTTGGATAATCAGAAACTGGTGCCTCAGACT CGTGTGCCCCCGGTCATCGTCCTGGAGAACCAAGGTCTCCGCTGGGTCCCTAAGGATAAG AACTTANTGATGTGGAGGGACTGGGAAGAGTCACGGCAGATGGTGGGAGCTCTACTGGAA GATCGGGCCCACCAGCACCTTGTGGACTTTGACTGCCACCTTGATGACATCCGGCAGGAC TGGACCAACCAGTGGCTCAACACTCAAATCAACCAGTGGGTTGGTCCCACTAATGGAAAA TGGAAATGCCTGAACCCAGGGCCATGCGGGACCCGTTTCCATTAAAGAGACTTCGGGCTG ACAAACAAAAACACCACACTAATCCAATATTTTCCTCGTATTTCAAATTGCCGGCCGCGG NCTTAACTTGTTATCTTGAACAAATCCCCGGGTTGTCTTCTCTGTGAACCCTTCACCAGG CCTTCTTCTGCCCTGGAAGAGGACATCCAATGCCCACACCTCTGGCTAATAAATAATTCG CTCATCTT >OriGene 3' read for NM_016049 unedited
GCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGCCCAA TTCTTTTTATTGGAACCGGGCCCCGTTGGCCCTGGCTAAGGCATTTCCATTTCCATTAGG GGGACCAACCCACTGGGTGATTTGAGTGTTGACCCGTTGGTTGGTCCATTCCTGCCGGAT GTCATCAAGGTGGCAGTCAAAGTCCACAAGGTGCTGGTGGGCCCGATCTTCCAGTAAAGC TCCCACCATTTGCCGGGATTTTTCCCAGTCCCTCCACATAACTAAGTTCTTATCCTTAGG GACCCAGCGGAGACCTTGGTTTTCCAGGACGATGACCGGGGGCACACGAGGCTGAGGCAC CAGTTTCTGATTATCCAACATAATAAGTACTGCTTCAGGGAAAAATTCTGCATTTCGCCC AGCATTTTTAAAGGCCAGGGGCCCAGGGTTTTGATCGTTCACAGTTGCTTTGGCATGGTA TTAACCAGCCACCCACCAGACCGGCCTGTGCTCCCCACACTTTCCCCTTGGTTGAGGGCG ACCTCCACCTGACGGACAGGGCCAGGTGGCTGTGGAATAGGGGCACACAGTCGGTGAGGC AAAGGTTTTCTCCAAACCGCGGGGCTGGGGCCAAAAACACCCCGTTGACTTGCGCGTGTG GGTTACCGGCAACATGCAGGCCATTTTTCCTATGGCCAGGGCCCGATATTCTCCACCTTC CCCATGGTAAGCGAGGTTTCTGGTTCGGGAACAACGCTTACTTCCATTCCTAGGTACCCC GCCGGTTCCCCCTCGGGCCGAATTCGCGGCCGCCTTATTTGGAGTCCGTTTCAAAACTCT GCACGGTAACTATAAGAACCTTTGTTTTATATATCTCCCCCGATCACCCCTCACGCGCTT TTGCGTTAACGGGCCGGTGTTTTTCAATTTTTTGGAATACCCGTTGTCTTGTGGCATAAN AACTTCATTTGCGACTTGAGGCGGGATCTCGTACACCCCG |
Restriction Sites | NotI-NotI |
ACCN | NM_016049 |
Insert Size | 800 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016049.3, NP_057133.2 |
RefSeq Size | 896 bp |
RefSeq ORF | 627 bp |
Locus ID | 51016 |
UniProt ID | Q9Y3B6 |
Domains | UPF0172 |
Gene Summary | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (a). |
Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200034 | EMC9 (Myc-DDK-tagged)-Human family with sequence similarity 158, member A (FAM158A) |
CNY 2,400.00 |
|
RC200034L3 | Lenti ORF clone of Human family with sequence similarity 158, member A (FAM158A), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200034L4 | Lenti ORF clone of Human family with sequence similarity 158, member A (FAM158A), mGFP tagged |
CNY 5,890.00 |
|
RG200034 | EMC9 (tGFP-tagged) - Human family with sequence similarity 158, member A (FAM158A) |
CNY 4,000.00 |