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ITCH (NM_031483) Human Untagged Clone
CAT＃: SC108233
ITCH (untagged)-Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH)
CNY 16,630.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADMFD; AIF4; AIP4; NAPP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_031483, the custom clone sequence may differ by one or more nucleotides
ATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTG TCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGT AGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACA GTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTT TGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAGACTTGTCAATTTGTCTTGAT GGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATG ATGATGGCTCCAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAGG AGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTTCT AGACCTCCAAGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACAAATACAAATACAAATACATC TGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAAT CCTGTAACTCAAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGAGTTTACTATG TAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGAACCTCTACCTCCTGGCTGGGAACGGCGGGT TGACAACATGGGACGTATTTATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCAAGGAGCAATGCAGCAGTTTA ACCAGAGATTCATTTATGGGAATCAAGATTTATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGG TCCATTGCCACCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAACCACAACACA CGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAATTAAATGAAAAGCCCTTACCTGAAGGTTGGG AAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATGTTCGGGACTTCAAAGCAAAG GTTCAGTATTTCCGGTTCTGGTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAA AAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAAGACGTTTGTG GGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCA CATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATTGGCAGATTTATTGCCATGGC TCTGTTCCATGGGAAATTCATAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCA GTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAGGAAAACA ATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCA TGATCTGAAACCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTGAAGGCTTTAATGAAATTC TTCCCCAGCAATATTTGCAATACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGAT TGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACAAATCATGTGG TTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCT GCCGATTGCCAGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCCTGGACCTGCCACCATACAAG AGCTATGAGCAACTGAAGGAAAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAA >OriGene 5' read for NM_031483 unedited
GGGCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGATGCATTTCA CAGTGGCCTTGTGGAGACAACGCCTTAACCCAAGGAAGTGACTCAAACTGTGAGAACTTC AGGTTTTCCAACCTATTGGTGGTATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTA GCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATA AGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGA AGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCG TTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATG TTTTGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAAC TTGAAGAAGTAGTTGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAG GAGACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTG AAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTCCAGATCCAAGGATGAAA CAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAAGAGCTGGTGAAAATAGGA GAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTCTAGACCTCA AGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGGTCACCA TCCTGCCACTTTGAAAGTGAGGGTCTAGTACAAGCTCTCCTGCCCCGACAAAACCAATAC AATACATCTGG >OriGene 3' read for NM_031483 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTATCCCCCACCACGCTGCCC AAAAAAAAGAACAGGGTCTCCCTATGTTGCCCAGGCTGGTCTTTAACTCCTGGGCTAAAG GGATCCTCCTGCCTCGGCCTCCCAGAGTGCTAGGATTACAGGTATGAGCCACCATGCCCG GCCCTAGGTTAACTTCTTAAAACAATGAAGGAATCACAGTGTTAACATTGTTTGTAAAGT GTAGTATTTGTCAGGCAACTGAAACAATCAGACTTCCAAATGAAGTCATTTGCAGGCTGT GACCAACTTTTCCCTGAATTCAAATTGACAAAACTACTTTGTAGAGATGCAAGAGTCTTG CCTCACTTCCAGGAATGATGTATGGATTCATGCGCCAGAAAGGAAAAACAATAGTCAAGT CAGACTAAAATGAGAGGGAGAACACAAGCCCGAATTTTGCTCACATCCNCAGAAGCAGGA GTAAGCTTGCACTTTTAACAAATGCCTCTTACCAAGAGGCTTAATAGGAAAATCTTGTAT ATTGCAGTTACCAAAGGACTTCCCTGCATGTCTGCAGAATGTCTAANAGAAAAAACACTA CTCCCAAGTGAGACTTTTGACACACATAANATATTGTGTTAAAGGAATAACTAGTGGAAT AGACAAGGGCAATTCATTGTGTGTAAAATCTATGTTAGCAGGTTCTGACTAGTCATTGCA GACCTGATTCTGGAACGCTGAGTGAAATCACTGGAGATAATCTAATGAGACATTATTTAC TTCACTCAGATACTCTCTAAGACCCATTGTCATTTACAGATGTGCCACAGCANAAAAGAC AAACATGCAATTAGTCCTGCCATTCATGTGCCAGTTTAAAGTACTCTGCCAATCCTCTGT TTTCTATGCAACAAAGCTTNCTAGTGCTAAACTCTGGTGGGGCAGTCAGCGATAAACGGT GACTCTGGACCATTTCTTCCACTTTCAGCAATTCTTGGCATGCTCCAGAAAACAACTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031483 |
| Insert Size | 3910 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031483.3, NP_113671.3 |
| RefSeq Size | 6357 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 83737 |
| UniProt ID | Q96J02 |
| Domains | C2, HECT, WW |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220408 | ITCH (Myc-DDK-tagged)-Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH) |
CNY 6,312.00 |
|
| RC220408L1 | Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), Myc-DDK-tagged |
CNY 8,712.00 |
|
| RC220408L2 | Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), mGFP tagged |
CNY 11,020.00 |
|
| RC220408L3 | Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), Myc-DDK-tagged |
CNY 11,020.00 |
|
| RC220408L4 | Lenti ORF clone of Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH), mGFP tagged |
CNY 11,020.00 |
|
| RG220408 | ITCH (tGFP-tagged) - Human itchy E3 ubiquitin protein ligase homolog (mouse) (ITCH) |
CNY 7,912.00 |
