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Nck (NCK1) (NM_006153) Human Untagged Clone
CAT＃: SC108190
NCK1 (untagged)-Human NCK adaptor protein 1 (NCK1), transcript variant 1
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NCK; nck-1; NCKalpha |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108190 sequence for NM_006153 edited (data generated by NextGen Sequencing)
ATGGCAGAAGAAGTGGTGGTAGTAGCCAAATTTGATTATGTGGCCCAACAAGAACAAGAG TTGGACATCAAGAAGAATGAGAGATTATGGCTTCTGGATGATTCTAAGTCCTGGTGGCGA GTTCGAAATTCCATGAATAAAACAGGTTTTGTGCCTTCTAACTATGTGGAAAGGAAAAAC AGTGCTCGGAAAGCATCTATTGTGAAAAACCTAAAGGATACCTTAGGCATTGGAAAAGTG AAAAGAAAACCTAGTGTGCCAGATTCTGCATCTCCTGCTGATGATAGTTTTGTTGACCCA GGGGAACGTCTCTATGACCTCAACATGCCCGCTTATGTGAAATTTAACTACATGGCTGAG AGAGAGGATGAATTATCATTGATAAAGGGGACAAAGGTGATCGTCATGGAGAAATGCAGT GATGGGTGGTGGCGTGGTAGCTACAATGGACAAGTTGGATGGTTCCCTTCAAACTATGTA ACTGAAGAAGGTGACAGTCCTTTGGGTGACCATGTGGGTTCTCTGTCAGAGAAATTAGCA GCAGTCGTCAATAACCTAAATACTGGGCAAGTGTTGCATGTGGTACAGGCTCTTTACCCA TTCAGCTCATCTAATGATGAAGAACTTAATTTCGAGAAAGGAGATGTAATGGATGTTATT GAAAAACCTGAAAATGACCCAGAGTGGTGGAAATGCAGGAAGATCAATGGTATGGTTGGT CTAGTACCAAAAAACTATGTTACCGTTATGCAGAATAATCCATTAACTTCAGGTTTGGAA CCATCACCTCCACAGTGTGATTACATTAGGCCTTCACTCACTGGAAAGTTTGCTGGCAAT CCTTGGTATTATGGCAAAGTCACCAGGCATCAAGCAGAAATGGCATTAAATGAAAGAGGA CATGAAGGGGATTTCCTCATTCGTGATAGTGAATCTTCGCCAAATGATTTCTCAGTATCA CTAAAAGCACAAGGGAAAAACAAGCATTTTAAAGTCCAACTAAAAGAGACTGTCTACTGC ATTGGGCAGCGTAAATTCAGCACCATGGAAGAACTTGTAGAACATTACAAAAAGGCACCA ATTTTTACAAGTGAACAAGGAGAAAAATTATATCTTGTCAAGCATTTATCATGA Clone variation with respect to NM_006153.4 >OriGene 5' read for NM_006153 unedited
TGTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCG AATTCGGCACGAGGCCGCCTCTCTCCCAAAAGCTACGCGGCGGCGGCGGAGCGCAGGCCT CGTGCCGTTACGGCCATCACGGCGGCCGCAGTGGCGTCCTGGAGCCCTCCTCAGTGCTGA AGCTGCTGAAAGATGGCAGAAGAAGTGGTGGTAGTAGCCAAATTTGATTATGTGGCCCAA CAAGAACAAGAGTTGGACATCAAGAAGAATGAGAGATTATGGCTTCTGGATGATTCTAAG TCCTGGTGGCGAGTTCGAAATTCCATGAATAAAACAGGTTTTGTGCCTTCTAACTATGTG GAAAGGAAAAACAGTGCTCGGAAAGCATCTATTGTGAAAAACCTAAAGGATACCTTAGGC ATTGGAAAAGTGAAAAGAAAACCTAGTGTGCCAGATTCTGCATCTCCTGCTGATGATAGT TTTGTTGACCCAGGGGAACGTCTCTATGACCTCAACATGCCCGCTTATGTGAAATTTAAC TACATGGCTGAGAGAGAGGATGAATTATCATTGATAAAGGGGACAAAGGTGATCGTCATG GAGAAATGCAGTGATGGGTGGTGGCGTGGTAGCTACAATGGACAAGTTGGATGGTTCCCT TCAAACTATGTAACTGAAGAAGGTGACAGTCCTTTGGGTGACCATGTGGNGTCTCTGTCA GAGAAATTAGCAGCAGTCGTCAATAACCTANATACTGGGCAAGTGTTGCATGTGGTACAG GCTCTTTACCCATTCAGCTCATCTAATGATGAAGAAACTTATTTCGAGAAAGGAGATGTA ATGGATGNTATTGAAAAACCCTGAAATGACCCACAGTGGTGGAAATGCANNGAGACAATG GTTTGGGTTGGTCTAGTACAAAAAC >OriGene 3' read for NM_006153 unedited
CTATGAACCGCGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGAAAAAAATAA GTGATTTTTATTTCCAACAGTTGTAAATTAAAATTGTACAACATGTTTTCTAATCTATGT CTCATGTGTCTTGCTGTATGACTGAAGACAATGAATGTATTTTTAAAAATTATGGCAGAC ACAATATACTTGGTATTTACAATGGCCTGTGACAAATATAATACCTGGAAATAATTTCTA ATTTTCCACTTATTATTGCTATTTAGTCACAGAAAAGATTAACACTCTCCAAGAAATTTT AGCATATGTCATATATAGATATAATAATTTTATATAATGAAAAACCATAACAGAACAGGG TGCCTTTACAAGGAAACAAAGGAAAGGCAATAAAGGATTTCTTCCATATAAAGATTCTGA TATGCATTATTTGATTTTTGTTTACAAAATACAAAACTGTAATATTAGAAGCAAAGGGAA AACAGCAAAGAAAAAAACAATAAAACAGAAGGCCAAGGATAAGGAACTGTTCTATGCAGA TGCACTGCATACATAGTATATATGTATGGGCTGAGTTATAATAAAAATACTTATTGTCAA TTCTCATATAGATTTAGAAACAACAATTTCCAATCAAGCACGACTGGACCCAACATTTTC TCAGTCTTCAATTACATGACAAATTACAGCTACACAGCAGTCACTTCTGGTCAGCAGTAT CATGATAAATGCTTGACAGATATAATTTTTCTCCTTGTTCACTTGTAAAAATTGGTGCCT TTTTGTAATGTTCTACAAGTTCTTCCATGGTGCTGAATTTACGCTGCCCAATGCAGTAGA CAGTCTCTTTTAGNTGGGACTTAAAATGCTGNNTTNCCCTTGNNGCTTTANGATACTGAG AAATCATTGCGAAGATCACTATCACGAATGAGAAATCCCTNCAGTCCTCTTCATTAATGC ATNCTGCTGATGCCTGGGACTTGCATATACAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006153 |
| Insert Size | 1910 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006153.3, NP_006144.1 |
| RefSeq Size | 1938 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 4690 |
| UniProt ID | P16333 |
| Domains | SH2, SH3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance, ErbB signaling pathway, Pathogenic Escherichia coli infection, T cell receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein (isoform 1). Variants 1 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202869 | NCK1 (Myc-DDK-tagged)-Human NCK adaptor protein 1 (NCK1), transcript variant 1 |
CNY 3,656.00 |
|
| RC202869L1 | Lenti ORF clone of Human NCK adaptor protein 1 (NCK1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC202869L2 | Lenti ORF clone of Human NCK adaptor protein 1 (NCK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202869L3 | Lenti ORF clone of Human NCK adaptor protein 1 (NCK1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202869L4 | Lenti ORF clone of Human NCK adaptor protein 1 (NCK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202869 | NCK1 (tGFP-tagged) - Human NCK adaptor protein 1 (NCK1), transcript variant 1 |
CNY 5,256.00 |
|
| SC320755 | NCK1 (untagged)-Human NCK adaptor protein 1 (NCK1), transcript variant 1 |
CNY 3,656.00 |
