sphingosine 1 phosphate phosphatase 1 (SGPP1) (NM_030791) Human Untagged Clone

CAT#: SC107997

SGPP1 (untagged)-Human sphingosine-1-phosphate phosphatase 1 (SGPP1)



  "NM_030791" in other vectors (7)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-SGPP1 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms SPP-1; SPPase1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC107997 sequence for NM_030791 edited (data generated by NextGen Sequencing)
ATGTCGCTGAGGCAGCGCCTGGCCCAGCTGGTTGGCCGTCTGCAGGACCCGCAGAAAGTG
GCCCGTTTCCAGCGGCTGTGCGGGGTGGAAGCGCCGCCGCGCCGCTCAGCAGACCGGAGG
GAGGATGAGAAAGCGGAGGCGCCTCTCGCCGGAGACCCTCGACTGCGAGGGCGGCAGCCA
GGGGCGCCTGGAGGCCCCCAGCCTCCCGGGAGCGACCGCAATCAGTGCCCGGCCAAGCCG
GACGGCGGCGGCGCCCCCAACGGCGTGCGGAACGGGCTGGCGGCCGAGCTGGGCCCGGCC
TCGCCGCGGCGCGCGGGCGCTCTGCGCCGCAACTCGCTGACGGGCGAGGAGGGCCAGCTG
GCCCGCGTGAGCAACTGGCCGCTCTACTGCCTGTTCTGCTTCGGCACGGAGCTGGGCAAC
GAACTCTTCTACATCCTGTTCTTCCCCTTCTGGATCTGGAACCTGGACCCTCTGGTGGGC
CGGAGGCTCGTGGTCATCTGGGTGCTGGTCATGTACCTGGGCCAGTGCACCAAGGACATC
ATCCGCTGGCCGAGGCCCGCCTCGCCGCCCGTGGTCAAGTTGGAGGTCTTCTACAACTCT
GAGTACAGCATGCCCTCCACCCATGCCATGTCCGGCACCGCCATCCCCATTTCTATGGTC
CTCCTCACCTATGGCCGCTGGCAGTACCCTCTTATATATGGACTGATTCTTATTCCCTGC
TGGTGTTCTCTAGTTTGCCTAAGTAGAATTTACATGGGAATGCACTCTATTCTGGATATT
ATTGCTGGATTCCTATATACCATTTTAATCTTAGCTGTCTTCTATCCATTTGTGGACCTG
ATTGACAACTTCAACCAAACTCACAAATATGCTCCATTCATCATCATCGGGCTTCATTTA
GCTTTGGGGATCTTTTCTTTCACTCTTGACACCTGGAGCACATCCCGAGGAGACACAGCC
GAGATACTAGGAAGTGGTGCTGGAATTGCATGTGGATCTCATGTTACTTATAACATGGGT
CTAGTATTAGATCCTTCTCTAGATACATTACCTTTAGCTGGGCCCCCCATTACTGTGACT
CTGTTTGGAAAAGCCATATTGCGGATCCTCATAGGGATGGTATTTGTACTAATAATCAGA
GATGTAATGAAAAAGATCACCATTCCTTTAGCCTGCAAAATCTTCAATATACCGTGTGAT
GATATTCGAAAAGCAAGACAGCACATGGAAGTTGAACTTCCTTATCGGTATATTACCTAT
GGAATGGTTGGTTTCTCCATCACATTTTTTGTTCCTTACATATTTTTCTTTATTGGTATC
TCTTGA

Clone variation with respect to NM_030791.2
>OriGene 5' read for NM_030791 unedited
NNNGGTTCGCAATTTTGTCATACGACTCCTATAGGCGGCCGCGNAATTCGCACCAGAAGC
GCTGTGCCGGTTCCGCTGCCGCTGGCCAGGAGCGCGGGGACAGTTCGGGGCTGCGCCGGA
GGCCGGCCCGCCTTCCCGGGGGTTCCGTTATCATGTCGCTGAGGCAGCGCCTGGCCCAGC
TGGTTGGCCGTCTGCAGGACCCGCAGAAAGTGGCCCGTTTCCAGCGGCTGTGCGGGGTGG
AAGCGCCGCCGCGCCGCTCAGCAGACCGGAGGGAGGATGAGAAAGCGGAGGCGCCTCTCG
CCGGAGACCCTCGACTGCGAGGGCGGCAGCCAGGGGCGCCTGGAGGCCCCCAGCCTCCCG
GGAGCGACCGCAATCAGTGCCCGGCCAAGCCGGACGGCGGCGGCGCCCCCAACGGCGTGC
GGAACGGGCTGGCGGCCGAGCTGGGCCCGGCCTCGCCGCGGCGCGCGGGCGCTCTGCGCC
GCAACTCGCTGACGGGCGAGGAGGGCCAGCTGGCCCGCGTGAGCAACTGGCCGCTCTACT
GCCTGTTCTGCTTCGGCACGGAGCTGGGCAACGAACTCTTCTACATCCTGTTCTTCCCCT
TCTGGATCTGGAACCTGGACCCTCTGGTGGGCCGGAGGCTCGTGGTCATCTGGGTGCTGG
TCATGTACCTGNGCCAGTGCACCAAGGACATCATCCGCTGGCCGAGGCCCGCCTCGCCGC
CCGTGGTCAAGTTGGAGGTCTTCTACAACTCTGAGTACAGCATGCCCTCCACCCATGCCA
TGTCCGGCACCGNCATCCCCATTTCTATGGTCCTNCTCACCTATGGCCGCTGGCAGTACC
CTCTTATATATGGACTGATTCTTATCCCTGCTGGNNNGTCTCTAGTTGCCTAAGTAGAAT
TACATGGGAATGCACTCTATTCTGGATATATNGCTGGATCCTATATAC
>OriGene 3' read for NM_030791 unedited
GAGCACAAATTATGNTACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTT
TAAACTTCAATTTGGGCTTTTAATTTTAAAATACATTTAGCATAGAACTGTCATAGGACA
GAAAATAATAAAACACAAATGGAACATTTTCAAATACCTTTTAAGCTAGACATAAAAATC
AACAGGATAGCATAGGCCCAAATTATGGGAAACGGTCCCTAAAATTCAATTCATTAAACA
TACATTTTGAAAGTAAAGCTCTTTTCACATTTTCCAACGTACCAATATTTTCCTACATGC
CTTGGTTTCCTTTTAACTAATAAGTAAAGGGTAAATTTAGTTGCTTTACTTAAAATTACC
AGCTTCAGTTTTGGTAAAAATTACCATGCCCCTAAATTCTCAATCAGAATTATAAAAATA
TGAGCTCAAATAGTTATGGTGCCCAAAATAGAAACATCTGAAGTGATAGTTCTGGATAAT
CATACAGATATTGCACTAAAATCAGTAATTTCATACCTGGTATTTATTTATATGGAAAGT
TAATCTCTTCTTTATGAAAAACTTTCTACAAATACACTTACTATTAACGTAAGTACAACC
ACACTTCAAAATATAAAGGTAGACAGAAAAAAGAATAGTTTAATACTATACGCAAATTTT
CCACTGTGAAAATAATAGAAAGTTGAAAAAATAATAGGATCTAAAATACTGATACTAATT
TTAGGTTTTATAATTTACCTTAAATAGCCAATAAATGGGTATTAAAAGTTAACGGTGCTA
AATCCAATCCTTCCAAATTATTTAGAAAATGCTTTGTTTACATATGAAGTATGTGCATTG
TCCCATCTATAAATTTCACGTTTCCATTCACTGG
Restriction Sites NotI-NotI     
ACCN NM_030791
Insert Size 3500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030791.2, NP_110418.1
RefSeq Size 3323 bp
RefSeq ORF 1326 bp
Locus ID 81537
UniProt ID Q9BX95
Domains acidPPc
Protein Families Druggable Genome, Phosphatase, Transmembrane
Protein Pathways Sphingolipid metabolism
Gene Summary Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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