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FZD3 (NM_017412) Human Untagged Clone
CAT＃: SC107940
FZD3 (untagged)-Human frizzled family receptor 3 (FZD3), transcript variant 1
CNY 7,328.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Fz-3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107940 sequence for NM_017412 edited (data generated by NextGen Sequencing)
ATGGCTATGACTTGGATTGTCTTCTCTCTTTGGCCCTTGACTGTGTTCATGGGGCATATA GGTGGGCACAGTTTGTTTTCTTGTGAACCTATTACCTTGAGGATGTGCCAAGATTTGCCT TATAATACTACCTTCATGCCTAATCTTCTGAATCATTATGACCAACAGACAGCAGCTTTG GCAATGGAGCCATTCCACCCTATGGTGAATCTGGATTGTTCTCGGGATTTCCGGCCTTTT CTTTGTGCACTCTACGCTCCTATTTGTATGGAATATGGACGTGTCACACTTCCCTGTCGT AGGCTGTGTCAGCGGGCTTACAGTGAGTGTTCGAAGCTCATGGAGATGTTTGGTGTTCCT TGGCCTGAAGATATGGAATGCAGTAGGTTCCCAGATTGTGATGAGCCATATCCTCGACTT GTGGATCTGAATTTAGCTGGAGAACCAACTGAAGGAGCCCCAGTGGCAGTGCAGAGAGAC TATGGTTTTTGGTGTCCCCGAGAGTTAAAAATTGATCCTGATCTGGGTTATTCTTTTCTG CATGTGCGTGATTGTTCACCTCCTTGTCCAAATATGTACTTCAGAAGAGAAGAACTGTCA TTTGCTCGCTATTTCATAGGATTGATTTCAATCATTTGCCTCTCGGCCACATTGTTTACT TTTTTAACTTTTTTGATTGATGTCACAAGATTCCGTTATCCTGAAAGGCCTATTATATTT TATGCAGTCTGCTACATGATGGTATCCTTAATTTTCTTCATTGGATTTTTGCTTGAAGAT CGAGTAGCCTGCAATGCATCCATCCCTGCACAATATAAGGCTTCCACAGTGACACAAGGA TCTCATAATAAAGCCTGTACCATGCTTTTTATGATACTCTATTTTTTTACTATGGCTGGC AGTGTATGGTGGGTAATTCTTACCATCACATGGTTTTTAGCAGCTGTGCCAAAGTGGGGT AGTGAAGCTATTGAGAAGAAAGCATTGCTGTTTCACGCCAGTGCATGGGGCATCCCCGGA ACTCTAACCATCATCCTTTTAGCGATGAATAAAATTGAAGGTGACAATATTAGTGGCGTG TGTTTTGTTGGCCTCTACGATGTTGATGCATTGAGATATTTTGTTCTTGCTCCCCTCTGC CTGTATGTGGTAGTTGGGGTTTCTCTCCTCTTAGCTGGCATTATATCCCTAAACAGAGTT CGAATTGAGATTCCATTAGAAAAGGAGAACCAAGATAAATTAGTGAAGTTTATGATCCGG ATCGGTGTTTTCAGCATTCTTTATCTCGTACCACTCTTGGTTGTAATTGGATGCTACTTT TATGAGCAAGCTTACCGGGGCATCTGGGAAACAACGTGGATACAAGAACGCTGCAGAGAA TATCACATTCCATGTCCATATCAGGTTACTCAAATGAGTCGTCCAGACTTGATTCTCTTT CTGATGAAATACCTGATGGCTCTCATAGTTGGCATTCCCTCTGTATTTTGGGTTGGAAGC AAAAAGACATGCTTTGAATGGGCCAGTTTTTTTCATGGTCGTAGGAAAAAAGAGATAGTG AATGAGAGCCGACAGGTACTCCAGGAACCTGATTTTGCTCAGTCTCTCCTGAGGGATCCA AATACTCCTATCATAAGAAAGTCAAGGGGAACTTCCACTCAAGGAACATCCACCCATGCT TCTTCAACTCAGCTGGCTATGGTGGATGATCAAAGAAGCAAAGCAGGAAGCATCCACAGC AAAGTGAGCAGCTACCACGGCAGCCTCCACAGATCACGTGATGGCAGGTACACGCCCTGC AGTTACAGAGGAATGGAGGAGAGACTACCTCATGGCAGCATGTCACGACTAACAGATCAC TCCAGGCATAGTAGTTCTCATCGGCTCAATGAACAGTCACGACATAGCAGCATCAGAGAT CTCAGTAATAATCCCATGACTCATATCACACATGGCACCAGCATGAATCGGGTTATTGAA GAAGATGGAACCAGTGCTTAA Clone variation with respect to NM_017412.3 >OriGene 5' read for NM_017412 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGATATGTTGGCCAAAT GTGCCCCATGTAATAAAATGAAAAGAAGAGACAAGATGATGTCATTTTCCCATATTGTGA AACCAAAAACAAACGCCTTTTGTGAGACCAAGCTAACAAACCTCTGACGGTGCGAAGAGT ATTTAACTGTTTGAAGAATTTAACAGTAAGATACAGAAGAAGTACCTTCGAGCTGAGACC TGCAGGTGTATAAATATCTAAAATACATATTGAATAGGCCTGATCATCTGAATCTCCTTC AGACCCAGGAAGGATGGCTATGACTTGGATTGTCTTCTCTCTTTGGCCCTTGACTGTGTT CATGGGGCATATAGGTGGGCACAGTTTGTTTTCTTGTGAACCTATTACCTTGAGGATGTG CCAAGATTTGCCTTATAATACTACCTTCATGCCTAATCTTCTGAATCATTATGACCAACA GACAGCAGCTTTGGCAATGGAGCCATTCCACCCTATGGTGAATCTGGATTGTTCTCGGGA TTTCCGGCCTTTTCTTTGTGCACTCTACGCTCCTATTTGTATGGAATATGGACGTGTCAC ACTTCCCTGTCGTANGCTGTGTCAGCGGGCTTACAGTGAGTGTTCGAAGCTCATGGAGAT GTTTGGTGTTCCTTGGCCTGAAGAATGGAATGCAGTAGGTTCCCAGATTGTGATGAGCCA TATCCTCGACTTGTGGATCTGAATNTAGCTGGAGAACCAACTGANAGAGCCCCAGTGGCA GTGCAAAGAGACTATGGTTTTTGGTGTCCCCGAGAGTTAAAAATGATCCTGATCTGGGNT ATTCTTTTCTGCATGTGCGTGATTGGTCACCTTCTTGTCAATATGTACTTCAAAAGAGAA GACTGTCATTTGCTCGCTTTNCATAGGNAATGATTCATCATTTGCTCTCGGNACCATGTT ACTTTTTAACTTTTGATGATGCACAGATCCGT >Forward primer walk for NM_017412 unedited
TGGTTGGTACTCATCTTGACTGGCATTATATCCCTAAACATAGTTCGAATTGAGATTCCA TTATAAATGGAGAACCAAGATAAATTAGTGAAGTTTATGATCCGGTTCGGTGTTTTCAGC ATTCTTTATCTCGTACCACTCTTGGTTGTAATTGGATGCTACTTTTATGAGCAAGCTTAC CGGGGCATCTGGGAAACAACGTGGATACAAGAACGCTGCAGAGAATATCACATTCCATGT CCATATCAGGTTACTCAAATGAGTCGTCCAGACTTGATTCTCTTTCTGATGAAATACCTG ATGGCTCTCATAGTTGGCATTCCCTCTGTATTTTGGGTTGGAAGCAAAAAGACATGCTTT GAATGGGCCAGTTTTTTTCATGGTCGTAGGAAAAAAGAGATAGTGAATGAGAGCCGACAG GTACTCCAGGAACCTGATTTTGCTCAGTCTCTCCTGAGGGATCCAAATACTCCTATCATA AGAAAGTCAAGGGGAACTTCCACTCAAGGAACATCCACCCATGCTTCTTCAACTCAGCTG GCTATGGTGGATGATCAAAGAAGCAAAGCATGAAGCATCCACAGCATAGTGAGCAGCTAC CACGGCAGCCTCCACAGATCACGTGATGGCAGGTACACGCCCTGCAGTTACAGAGGAATG GAGGAGAGACTACCTCATGGCAGCATGTCACGACTAACAGATCACTCCCAGCATAGTAGT TCTCATCGGCTCAATGAACAGTCACGACATAGCAGCATCAGAGATCCTCAGTATAATCCC ATGACTCATATCACACATGGCACCAGCATGAATCGGGTTATTGAAAGAGATGGAACCAGT NGCTNAATTNGNNCTGNCTAAAGGAGGAAATCTGGTGCTGNTTAAAAAAGCAGATTTATT CCTTGNCTTTTGCATGACTGATAGCTGGTACTNACAAGTAACATGCTTTCAGTCAAGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017412 |
| Insert Size | 3900 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017412.2, NP_059108.1 |
| RefSeq Size | 3933 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 7976 |
| UniProt ID | Q9NPG1 |
| Domains | FRI, Frizzled |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
| Gene Summary | This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer
,Y Kawano, S Diez, P Uysal-Onganer, R S Darrington, J Waxman, R M Kypta,
British Journal of Cancer 100, 1165-1174 (10 March 2009)
[FZD3]
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R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6
,ME Binnerts, KA Kim, JM Bright, SM Patel, K Tran, M Zhou, JM Leung, Y Liu, WE Lomas 3rd, M Dixon, SA Hazell, M Wagle, WS Nie, N Tomasevic, J Williams, X Zhan, MD Levy, WD Funk, and A Abo,
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14700-5.
[FZD3]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214553 | FZD3 (Myc-DDK-tagged)-Human frizzled family receptor 3 (FZD3), transcript variant 1 |
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| RC214553L1 | Lenti ORF clone of Human frizzled family receptor 3 (FZD3), transcript variant 1, Myc-DDK-tagged |
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| RC214553L2 | Lenti ORF clone of Human frizzled family receptor 3 (FZD3), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
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| RC214553L3 | Lenti ORF clone of Human frizzled family receptor 3 (FZD3), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC214553L4 | Lenti ORF clone of Human frizzled family receptor 3 (FZD3), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG214553 | FZD3 (tGFP-tagged) - Human frizzled family receptor 3 (FZD3), transcript variant 1 |
CNY 6,370.00 |
