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DFFB (NM_004402) Human Untagged Clone
CAT＃: SC107911
DFFB (untagged)-Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB)
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAD; CPAN; DFF-40; DFF2; DFF40 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107911 sequence for NM_004402 edited (data generated by NextGen Sequencing)
ATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCTGCGCAGCCCGAGGAAGTTCGGC GTGGCTGGCCGGAGCTGCCAGGAGGTGCTGCGCAAGGGCTGTCTCCGCTTCCAGCTCCCT GAGCGCGGTTCCCGGCTGTGCCTGTACGAGGATGGCACGGAGCTGACGGAAGATTACTTC CCCAGTGTTCCCGACAACGCCGAGCTGGTGCTGCTCACCTTGGGCCAGGCCTGGCAGGGC TATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCACGAGCCACAGGTGGGGCTCATC CAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCCACAGAGGCAGAGGCTGCTGGCT GACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGAGACCCGGGCTGAGGACCCGCCG TGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTCTGGCTATCTGAGATACAGCTGT GAGAGCCGGATCCGGAGTTACCTGAGGGAGGTGAGCTCCTACCCCTCCACGGTGGGTGCG GAGGCTCAGGAGGAATTCCTGCGGGTCCTCGGCTCCATGTGCCAGAAGCTCCGGTCCATG CAGTACAATGGCAGCTACTTCGACAGAGGAGCCAAGGGCGGCAGCCGCCTCTGCACACCG GAAGGCTGGTTCTCCTGCCAGGGTCCCTTTGACATGGACAGCTGCTTATCAAGACACTCC ATCAACCCCTACAGTAACAGGGAGAGCAGGATCCTCTTCAGCACCTGGAACCTGGATCGC ATAATAGAAAAGAAACGCACCATCATTCCTACACTGGTGGAAGCAATTAAGGAACAAGAT GGAAGAGAAGTGGACTGGGAGTATTTTTATGGCCTGCTTTTTACCTCAGAGAACCTAAAA CTAGTGCACATTGTCTGCCATAAGAAAACCACCCACAAGCTCAACTGTGACCCAAGCAGA ATCTACAAACCCCAGACAAGGTTGAAGCGGAAGCAGCCTGTGCGGAAACGCCAGTGA Clone variation with respect to NM_004402.2 587 g=>a;779 a=>g >OriGene 5' read for NM_004402 unedited
AAAAAAAGGGGANNNANAANNAAAAACACCCCCCCGGTTCACAATTGTATACGACTCATA TAGGCGGCCGCGNAATTCGCACGAGGCCAGCTTGCAGAGCTCACCAGGTGCAGACCCCTG CGGCCAGGGCGAGGACGGATCTGAGCAGCTGGGCAGCAGGTGCCACCGCCTGTGGGACCC AGAGGGCTTGAGGACATCTGCAATGCTCCAGAAGCCCAAGAGCGTGAAGCTGCGGGCCCT GCGCAGCCCGAGGAAGTTCGGCGTGGCTGGCCGGAGCTGCCAGGAGGTGCTGCGCAAGGG CTGTCTCCGCTTCCAGCTCCCTGAGCGCGGTTCCCGGCTGTGCCTGTACGAGGATGGCAC GGAGCTGACGGAAGATTACTTCCCCAGTGTTCCCGACAACGCCGAGCTGGTGCTGCTCAC CTTGGGCCAGGCCTGGCAGGGCTATGTGAGCGACATCAGGCGCTTCCTCAGTGCATTTCA CGAGCCACAGGTGGGGCTCATCCAGGCCGCCCAGCAGCTGCTGTGTGATGAGCAGGCCCC ACAGAGGCAGAGGCTGCTGGCTGACCTCCTGCACAACGTCAGCCAGAACATCGCGGCCGA GACCCGGGCTGAGGACCCGCCGTGGTTTGAAGGCTTGGAGTCCCGATTTCAGAGCAAGTC TGGCTATCTGAGATACAGCTGTGAGAGCCCNATCCGGAGTTACCTGNAGGAGGTGAGCTC CTACCCCTCCCACGTGGGTGCGGAGGCTCCAGAGGAATTCCTGCCGCTCCTCCGCTCCAT GGGCCANAAGCTTCCGTCCATGCAGTACAATGGCAGCTACTTCCACAGAGGAGCCAAGGC CGGCAGCCGCCTTTGCACACCGGAAGGGCTGTTCTCCCTGCCGGGTCCCTTTGACAGGAA CAGCTGCTTAAN >OriGene 3' read for NM_004402 unedited
NNTTTCTGTGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTATTTT TGAAAAATCTTTATTGCTTTTCCTGATTGTACAACAAATGTAAAAATTCAAATATTACAA AATGACTTAATTTAAAAAGTACATTTTAACATGTTATAAAATAAATGCCCATTTCAAAAA ATTCAAGTATCATAAAATACATGACGTAGAAAATGAGTCCTCTGTAATCTAGCCCAACCC CCGTATCCCCCAGGATTACTGCTGTTCAGATCCGCGTGTTTCCTTCCAGACCGTCTCGTG GTGNCNNNNNACCCNCCCGGNCCNCCCAACCCCCCCAACCAACGGGGCCTTGGACCATGA AGCCTTCCATATTGAATCCCGNAGNATGAAATAGTTAACCGCTGAACCCAACGGAAGCCA AGGGTTTCAANCTGCCCGGATGCCCCCTGGAAACAAGCTTCCCAGTTTTCCTTAACACCA GAAAACCCCTTTTTCCCTTCAATAAAAAAACCCTTGGAAAAACCTGGGGGAACACCAAAT TTTTTTGGCCCCTTTTGGTAAAATGGTATTCAAGGGTTGGAAATCCCTTGAAAAATAAAA TGGGATACCCCTTAACCCATTTTTTTTGGGCCCCTTAAAAAAAAAAAACAAGAGTTTTCC CCCGGGGGTTCAAAACGGGGAAAAAAACTTTGGTTTTCAAAAAAAAAACAATTTGGGGGG GGGGGGGTTTTCCCCCTTTTTTTTCTCTGTGGCCCCTTTTTTTAATAAAACAAATTTTTC AACCCGGGGGGGTCACGAGGGGGCCCCCACGGGGGGGGTTTTAAAAGCCCCCCCGGTGAT AAACCCCCCCCCACCACCCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004402 |
| Insert Size | 3340 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004402.2, NP_004393.1 |
| RefSeq Size | 3034 bp |
| RefSeq ORF | 1017 bp |
| Locus ID | 1677 |
| UniProt ID | O76075 |
| Domains | CAD |
| Protein Families | Druggable Genome |
| Protein Pathways | Apoptosis |
| Gene Summary | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (2) contains two consecutive alternate splice junctions compared to variant 1. The resulting isoform (2) has the same N- and C-termini but lacks an internal segment compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Caspase-dependent cell death-associated release of nucleosome and damage-associated molecular patterns
,Yoon, S;Park, SJ;Han, JH;Kang, JH;Kim, JH;Lee, J;Park, S;Shin, HJ;Kim, K;Yun, M;Chwae, YJ;,
Cell Death Dis
,PubMed ID 25356863
[DFFB]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208266 | DFFB (Myc-DDK-tagged)-Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB) |
CNY 3,656.00 |
|
| RC208266L1 | Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208266L2 | Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), mGFP tagged |
CNY 5,890.00 |
|
| RC208266L3 | Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208266L4 | Lenti ORF clone of Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), mGFP tagged |
CNY 5,890.00 |
|
| RG208266 | DFFB (tGFP-tagged) - Human DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB) |
CNY 5,256.00 |
