UBE1C (UBA3) (NM_198195) Human Untagged Clone
CAT#: SC107764
UBA3 (untagged)-Human ubiquitin-like modifier activating enzyme 3 (UBA3), transcript variant 2
CNY 3,656.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | hUBA3; NAE2; UBE1C |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC107764 sequence for NM_198195 edited (data generated by NextGen Sequencing)
ATGGCGGATGGCGAGGAGCCAATGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGG GAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCT GATTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAGTTCTAGTC ATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTGGCCTTGTCTGGTTTTAGA CAGATTCATGTTATAGATATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTA TTTAGGCCTAAAGATATTGGAAGACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGAC AGAGTTCCTAATTGCAATGTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACT TTCTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCAGAAGATGG ATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGTGTCTTAGATCCAAGCTCC ATTGTCCCTTTGATAGATGGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGTGATTCTG CCTGGAATGACTGCTTGTATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATTTT CCCATGTGCACCATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGG ATGTTGCAGTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGATGGAGATGAT CCTGAACATATACAATGGATTTTCCAAAAATCCCTAGAGAGAGCATCACAATATAATATT AGGGGTGTTACGTATAGGCTCACTCAAGGGGTAGTAAAAAGAATCATTCCTGCAGTAGCT TCCACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGT GCATACATTCCCTTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATAC ACATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCAAAATATT CAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTATCTAACCAATAGTGCTTCT CTGCAAATGAAATCTCCAGCCATCACAGCCACCCTAGAGGGAAAAAATAGAACACTTTAC TTACAGTCGGTAACCTCTATTGAAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAA GAATTGGGGCTTGTTGATGGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACT GTACTATTCAAACTTCATTTTACTTCTTAA Clone variation with respect to NM_198195.1 >OriGene 5' read for NM_198195 unedited
NGGGTTCGGAATTTGTNATACGACTCCTCTNAGGCGGCCGCGNAATTCGCACGAGGACAA TATGGCGGATGGCGAGGAGCCATGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGG GAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCT GATTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAGTTCTAGTC ATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTGGCCTTGTCTGGTTTTAGA CAGATTCATGTTATAGATATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTA TTTAGGCCTAAAGATATTGGAAGACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGAC AGAGTTCCTAATTGCAATGTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACT TTCTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCAGAAGATGG ATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGTGTCTTAGATCCAAGCTCC ATTGTCCCTTTGATAGATGGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGTGATTCTG CCTGGAATGACTGCTTGTATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATTTT CCCATGTGCACCATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGG ATGTTGCAGTGGCCTAAGAGCAGCCTTTTGGAAAGGNGTTNCATTAGATGGAGAGATCCT GAACATTACATGGGATTTCCAAAAATCCCTAGANAAGCATN >OriGene 3' read for NM_198195 unedited
GCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTACACAATATATGATTTTATTAA TAAATAGTGCAAAAGCATCAGTGATAACTGTTTGAACATTAAATTTTTTAAACAGCCATG TCTTGGCATTAGTTAATATTGTGCATATTGGCCTCTATGGCACTACAAGTAAACAGATGA AAATATTGCCCATTTTCATGCACAGGTATTCAGCTATAACACCATTTACAAATCATTATG AACAAGATAAATTCTGCAATAATATTCATTTGGATGGCCACAATTAAATGAGTGTTATAT CAAGAAATAGCCTATGTTCAATATACTCCAGATGTCAGATTGTAAAATGTAATGTTATTT AAAACTTAATTCTTTATTTTCCTTAAAGGGACACCTTTTGTGTATTTGGGTACTCAAATG AAAACTTAAGAATGCATTCTTTGACCATAATAACAAAATTCACACAAAAGAGGTTGTATG CTTCCTCCTCTAAAAGAAGCAATACATTTGCTCATAATCTCTCTCTCCAGGTATCATTTC TCATATTATTAATGAAAATGCTTACAAGCACCAACACCAAAATTCTGTCTTCAAGGGAAG GTTACAAAGTTAAAAAAGAGTGGTTCATTATATAAAAAAAGACAAATCGTGGCAACACTA TTGCTAAAAATGACATCGATTCAACTTCTTAGCATCCACAAAGTATATTATTTCCATGAG TTCTCTATTATGTGGAGATCCTCCTTAAGAGTAAAATGAAGTTTGATAGTACAGTCTGTG GAGTGGTGACATCAGCAACCGCCAGTTCTTGTCCATCAACAAGCCCCAATTNCTTCAATG TTTTGGAGAGAATTAGCCCTTGNTCGTTCTTCAATAGAAGGTACCGACTGNTAGTAAAAG TGTCTATTNTTTCCCTCTAGGNAGGCTGTGATGGCTGGAGATTNCATTGCAGAAAGCACT ATTGGNTAGATATCCAAACCTNCCGTAGTTAAN |
Restriction Sites | NotI-NotI |
ACCN | NM_198195 |
Insert Size | 2010 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_198195.1, NP_937838.1 |
RefSeq Size | 2094 bp |
RefSeq ORF | 1350 bp |
Locus ID | 9039 |
UniProt ID | Q8TBC4 |
Protein Pathways | Ubiquitin mediated proteolysis |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, as compared to variant 1. The encoded isoform (2) is thus missing an internal segment in the N-terminal region, as compared to isoform 1. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216801 | UBA3 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 3 (UBA3), transcript variant 2 |
CNY 3,656.00 |
|
RC216801L3 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 3 (UBA3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216801L4 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 3 (UBA3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG216801 | UBA3 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 3 (UBA3), transcript variant 2 |
CNY 5,256.00 |