EIF5 (NM_183004) Human Untagged Clone
CAT#: SC107610
EIF5 (untagged)-Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | EIF-5; EIF-5A |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_183004, the custom clone sequence may differ by one or more nucleotides
ATGTCTGTCAATGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTCTGATTGCCA AGGTTGAGGGCAAAGGCAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAA TCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAG AATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTA AAAAATTTGTTCTCTGTCCTGAATGTGAGAATCCTGAAACAGATTTGCATGTCAATCCAAAGAAGCAAAC AATAGGTAATTCTTGTAAAGCCTGTGGCTATCGAGGCATGCTTGACACACATCATAAACTCTGCACATTC ATTCTCAAAAACCCACCTGAGAATAGTGACAGTGGTACAGGAAAGAAAGAAAAAGAAAAGAAAAACAGAA AGGGCAAAGACAAGGAAAATGGCTCCGTATCCAGCAGTGAGACACCACCACCACCACCACCACCAAATGA AATTAATCCTCCTCCACATACAATGGAAGAAGAGGAGGATGATGACTGGGGAGAAGATACAACTGAGGAA GCTCAAAGGCGTCGAATGGATGAAATCAGTGACCATGCAAAAGTTCTGACACTCAGTGATGATTTGGAAA GAACAATTGAGGAGAGGGTCAATATCCTCTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGTGTTATTGA TTCATCTGACAAAGAAATCGTTGCTGAAGCAGAAAGACTGGATGTAAAAGCCATGGGCCCTCTTGTTCTA ACTGAAGTTCTTTTTAATGAGAAGATTAGAGAACAGATTAAGAAATACAGGCGCCATTTCCTACGATTTT GTCACAACAACAAAAAAGCCCAACGGTACCTTCTTCATGGTTTGGAGTGTGTGGTAGCAATGCATCAAGC TCAGCTTATCTCCAAGATTCCACATATCTTGAAGGAGATGTACGATGCAGACCTTTTAGAAGAAGAGGTC ATCATCAGCTGGTCGGAAAAGGCCTCTAAGAAATATGTCTCCAAAGAACTTGCCAAAGAGATTCGTGTCA AAGCAGAACCATTTATAAAATGGTTGAAGGAGGCAGAGGAAGAATCTTCTGGTGGCGAAGAAGAAGATGA AGATGAGAACATTGAGGTGGTGTATTCGAAGGCTGCCAGTGTACCGAAAGTTGAGACTGTAAAGTCAGAC AACAAGGATGACGACATCGATATTGATGCCATTTAA >OriGene 5' read for NM_183004 unedited
GGGGGCACTGTATTCGGCACCAGCTGGGGCCGAGGGTAGCTTGAGCGCGGCGGCGGCGTT GTTCAGTCAGAGCGAGAACATTCCAGAGGTCGCCCGGCCGCTCCTCGCTGCGCTTCGCCT CCGCCTCCTCGGACTCGGACTCGGGTTTATATCGCGCCTCACTTCATCCCAGTCCCGGGC GAGCAGCGTTGGGTTTATGGCTCCATTTGACGAAAACGAGCTGTTGCGCAGCCATTGGTA CCTGTATTGGGGAAACATAGCATACAAGCAAGAAGCTTACAGCCTCAGTGGCGAAAATTT TTTCATGTCAGAGACCGAGAACTCTTGCAGTCGTTTATGTCATCCCTTCTTCTCCAGACA GAAGATACCAAAAAGTTGCAATCAAAGATCTCTTCATCTTATTGATAAAGCCACTAATAA GCCAAAATGTCTGTCAATGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATG CCCCGTCTGATTGCCAAGGTTGAGGGCAAAGGCAATGGAATCAAGACAGTTATAGTCAAC ATGGTTGACGTTGCAAAGGCGCTTAATCGGCCTCCAACGTATCCCACCAAATATTTTGGT TGTGAGCTGGGAGCACAGACCCAGTTTTGATGTTAAGAATGACCGTTACATTGTCAATGG ATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTC |
Restriction Sites | NotI-NotI |
ACCN | NM_183004 |
Insert Size | 4700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_183004.3, NP_892116.2 |
RefSeq Size | 5782 bp |
RefSeq ORF | 1296 bp |
Locus ID | 1983 |
UniProt ID | P55010 |
Gene Summary | Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213587 | EIF5 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2 |
CNY 3,656.00 |
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RC213587L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
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RC213587L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
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RG213587 | EIF5 (tGFP-tagged) - Human eukaryotic translation initiation factor 5 (EIF5), transcript variant 2 |
CNY 4,370.00 |