PHD4 (P4HTM) (NM_177938) Human Untagged Clone

CAT#: SC107589

P4HTM (untagged)-Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 3



  "NM_177938" in other vectors (5)

CNY 9,120.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal PHD4/HIF Prolyl Hydroxylase 4 Antibody
    • 100 ug

CNY 5,302.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EGLN4; HIDEA; HIFPH4; P4H-TM; PH-4; PH4; PHD4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_177938, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCAGCGGCGGTGACAGGCCAGCGGCCTGAGACCGCGGCGGCCGAGGAGGCCTCGAGGCCGCAGT
GGGCGCCGCCAGACCACTGCCAGGCTCAGGCGGCGGCCGGGCTGGGCGACGGCGAGGACGCACCGGTGCG
TCCGCTGTGCAAGCCCCGCGGCATCTGCTCGCGCGCCTACTTCCTGGTGCTGATGGTGTTCGTGCACCTG
TACCTGGGTAACGTGCTGGCGCTGCTGCTCTTCGTGCACTACAGCAACGGCGACGAAAGCAGCGATCCCG
GGCCCCAACACCGTGCCCAGGGCCCCGGGCCCGAGCCCACCTTAGGTCCCCTCACCCGGCTGGAGGGCAT
CAAGGTGGGGCACGAGCGTAAGGTCCAGCTGGTCACCGACAGGGATCACTTCATCCGAACCCTCAGCCTC
AAGCCGCTGCTCTTCGAAATCCCCGGCTTCCTGACTGATGAAGAGTGTCGGCTCATCATCCATCTGGCGC
AGATGAAGGGGTTACAGCGCAGCCAGATCCTGCCTACTGAAGAGTATGAAGAGGCAATGAGCACTATGCA
GGTCAGCCAGCTGGACCTCTTCCGGCTGCTGGACCAGAACCGTGATGGGCACCTTCAGCTCCGTGAGGTT
CTGGCCCAGACTCGCCTGGGAAATGGATGGTGGATGACTCCAGAGAGCATTCAGGAGATGTACGCCGCGA
TCAAGGCTGACCCTGATGGTGACGGAGTGCTGAGTCTGCAGGAGTTCTCCAACATGGACCTTCGGGACTT
CCACAAGTACATGAGGAGCCACAAGGCAGAGTCCAGTGAGCTGGTGCGGAACAGCCACCATACCTGGCTC
TACCAGGGTGAGGGTGCCCACCACATCATGCGTGCCATCCGCCAGAGGGTGCTGCGCCTCACTCGCCTGT
CGCCTGAGATCGTGGAGCTCAGCGAGCCGCTGCAGGTTGTTCGATATGGTGAGGGGGGCCACTACCATGC
CCACGTGGACAGTGGGCCTGTGTACCCAGAGACCATCTGCTCCCATACCAAGCTGGTAGCCAACGAGTCT
GTACCCTTCGAGACCTCCTGCCGGCAAGTATCTCCCAACTGGGGGCTGCCTTCAATCCTCAGACCAGGAA
CACCCATGACACAGGCACAGCCCTGCACTGTGGGCGTGCCCCTTGGCATGGGGCCAGGAGATCACTGGGT
TATCCCGGTAAGCCCCTGGGAGCATCCACAACTGGGGACCTGCTCAGTGCCCCCCCTGCCTTACAGCTAC
ATGACAGTGCTGTTTTATTTGAACAACGTCACTGGTGGGGGCGAGACTGTTTTCCCTGTAGCAGATAACA
GAACCTACGATGAAATGAGTCTGATTCAGGATGACGTGGACCTCCGTGACACACGGAGGCACTGTGACAA
GGGAAACCTGCGTGTCAAGCCCCAACAGGGCACAGCAGTCTTCTGGTACAACTACCTGCCTGATGGGCAA
GGTTGGGTGGGTGACGTAGACGACTACTCGCTGCACGGGGGCTGCCTGGTCACGCGCGGCACCAAGTGGA
TTGCCAACAACTGGATTAATGTGGACCCCAGCCGAGCGCGGCAAGCGCTGTTCCAACAGGAGATGGCCCG
CCTTGCCCGAGAAGGGGGCACCGACTCACAGCCCGAGTGGGCTCTGGACCGGGCCTACCGCGATGCGCGC
GTGGAACTCTGA


>OriGene 5' read for NM_177938 unedited
GGGTGCAGATTTTGTATACGATTCATATAGGGCGGCCGCGAATTCGCACCAGGCCATGGC
GGCAGCGGCGGTGACAGGCCAGCGGCCTGAGACCGCGGCGGCCGAGGAGGCCTCGAGGCC
GCAGTGGGCGCCGCCAGACCACTGCCAGGCTCAGGCGGCGGCCGGGCTGGGCGACGGCGA
GGACGCACCGGTGCGTCCGCTGTGCAAGCCCCGCGGCATCTGCTCGCGCGCCTACTTCCT
GGTGCTGATGGTGTTCGTGCACCTGTACCTGGGTAACGTGCTGGCGCTGCTGCTCTTCGT
GCACTACAGCAACGGCGACGAAAGCAGCGATCCCGGGCCCCAACACCGTGCCCAGGGCCC
CGGGCCCGAGCCCACCTTAGGTCCCCTCACCCGGCTGGAGGGCATCAAGGTGGGGCACGA
GCGTAAGGTCCAGCTGGTCACCGACAGGGATCACTTCATCCGAACCCTCAGCCTCAAGCC
GCTGCTCTTCGAAATCCCCGGCTTTCTGACTGATGAAGAGTGTCGGCTCATCATCCATCT
GGCGCAGATGAAGGGGTTACAGCGCAGCCAGATCCTGCCTACTGAAGAGTATGAAGAGGC
AATGAGCACTATGCAGGTCAGCCAGCTGGACCTTTCCGGCTGCTGGACCAGACCGTGATG
GGCACCTTCAGCTCCGTGAGTTTCTGCCCAGACTCGCTGGGAAAGGGATGGTGGATGGAC
TCCGAGAGCATCCAGGAGATGTCGCCGCGATTCAGGCTGAACCTGATGGTGACGGTGAGC
TACAACCTTTGGAAAGTCCTTTCCGTGGACCTTCTGGCCCTTCCCAGAGGGCTGAGTCTG
CGGAATTTTCCAACTGGACCTTCGGGACTTCCCAAGTCCTGGAGGGCACAGGGAAATTCA
GTGGG
Restriction Sites NotI-NotI     
ACCN NM_177938
Insert Size 2750 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177938.2, NP_808807.2
RefSeq Size 2294 bp
RefSeq ORF 1692 bp
Locus ID 54681
UniProt ID Q9NXG6
Protein Families Druggable Genome, Transmembrane
Gene Summary The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) contains two additional segments in the coding region, compared to variant 1. The resulting isoform (c) is longer, compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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