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TXNRD1 (NM_182729) Human Untagged Clone
CAT＃: SC107562
TXNRD1 (untagged)-Human thioredoxin reductase 1 (TXNRD1), transcript variant 3 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
CNY 6,688.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Synonyms | GRIM-12; TR; TR1; TRXR1; TXNR |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_182729, the custom clone sequence may differ by one or more nucleotides
ATGAACGGCCCTGAAGATCTTCCCAAGTCCTATGACTATGACCTTATCATCATTGGAGGTGGCTCAGGAG GTCTGGCAGCTGCTAAGGAGGCAGCCCAATATGGCAAGAAGGTGATGGTCCTGGACTTTGTCACTCCCAC CCCTCTTGGAACTAGATGGGGTCTCGGAGGAACATGTGTGAATGTGGGTTGCATACCTAAAAAACTGATG CATCAAGCAGCTTTGTTAGGACAAGCCCTGCAAGACTCTCGAAATTATGGATGGAAAGTCGAGGAGACAG TTAAGCATGATTGGGACAGAATGATAGAAGCTGTACAGAATCACATTGGCTCTTTGAATTGGGGCTACCG AGTAGCTCTGCGGGAGAAAAAAGTCGTCTATGAGAATGCTTATGGGCAATTTATTGGTCCTCACAGGATT AAGGCAACAAATAATAAAGGCAAAGAAAAAATTTATTCAGCAGAGAGATTTCTCATTGCCACTGGTGAAA GACCACGTTACTTGGGCATCCCTGGTGACAAAGAATACTGCATCAGCAGTGATGATCTTTTCTCCTTGCC TTACTGCCCGGGTAAGACCCTGGTTGTTGGAGCATCCTATGTCGCTTTGGAGTGCGCTGGATTTCTTGCT GGTATTGGTTTAGACGTCACTGTTATGGTTAGGTCCATTCTTCTTAGAGGATTTGACCAGGACATGGCCA ACAAAATTGGTGAACACATGGAAGAACATGGCATCAAGTTTATAAGACAGTTCGTACCAATTAAAGTTGA ACAAATTGAAGCAGGGACACCAGGCCGACTCAGAGTAGTAGCTCAGTCCACCAATAGTGAGGAAATCATT GAAGGAGAATATAATACGGTGATGCTGGCAATAGGAAGAGATGCTTGCACAAGAAAAATTGGCTTAGAAA CCGTAGGGGTGAAGATAAATGAAAAGACTGGAAAAATACCTGTCACAGATGAAGAACAGACCAATGTGCC TTACATCTATGCCATTGGCGATATATTGGAGGATAAGGTGGAGCTCACCCCAGTTGCAATCCAGGCAGGA AGATTGCTGGCTCAGAGGCTCTATGCAGGTTCCACTGTCAAGTGTGACTATGAAAATGTTCCAACCACTG TATTTACTCCTTTGGAATATGGTGCTTGTGGCCTTTCTGAGGAGAAAGCTGTGGAGAAGTTTGGGGAAGA AAATATTGAGGTTTACCATAGTTACTTTTGGCCATTGGAATGGACGATTCCGTCAAGAGATAACAACAAA TGTTATGCAAAAATAATCTGTAATACTAAAGACAATGAACGTGTTGTGGGCTTTCACGTACTGGGTCCAA ATGCTGGAGAAGTTACACAAGGCTTTGCAGCTGCGCTCAAATGTGGACTGACCAAAAAGCAGCTGGACAG CACAATTGGAATCCACCCTGTCTGTGCAGAGGTATTCACAACATTGTCTGTGACCAAGCGCTCTGGGGCA AGCATCCTCCAGGCTGGCTGCTGAGGTTAA >OriGene 5' read for NM_182729 unedited
AGTTTTCTTCACTCCGGCATTTGCAGCAGAGCGAAAGGTGGTCGAGTCCTGAAGGAGGGC CTGATGTCTTCATCATTCTCAAATTCTTAGGACGGTCGGGCCCTGGAAGGAACGCTCTCG GAATTGGCCGCGGAAACCGATCTGCCCGTTGTGTTTGTGAAACAGAGAAAGATAGGCGGC CATGGTCCAACCTTGAAGGCTTATCAGGAGGGCAGACTTCAAAAGCTACTAAAAATGAAC GGCCCTGAAGATCTTCCCAAGTCCTATGACTATGACCTTATCATCATTGGAGGTGGCTCA GGAGGTCTGGCAGCTGCTAAGGAGGCAGCCCAATATGGCAAGAAGGTGATGGTCCTGGAC TTTGTCACTCCCACCCCTCTTGGAACTAGATGGGGTCTCGGAGGAACATGTGTGAATGTG GGTTGCATACCTAAAAAACTGATGCATCAAGCAGCTTTGTTAGGACAAGCCCTGCAAGAC TCTCGAAATTATGGATGGAAAGTCGAGGAGACAGTTAAGCATGATTGGGACAGAATGATA GAAGCTGTACAGAATCACATTGGCTCTTTGAATTGGGGCTACCGAGTAGCTCTGCGGGAG AAAAAAGTCGTCTATGAGAATGCTTATGGGCAATTTATTGGGTCCTCACAGGATTAAGGC AACAAATAATAAAGGCAAAGAAAAAATTTATTCAGCAGAGAGATTTCTCATTGNCACTGG TGAAAGAACACGTTACTTGGGCATCCCTGGGACAAAAATACTGCATCACAGGGAAGATC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_182729 |
| Insert Size | 3600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182729.1, NP_877393.1 |
| RefSeq Size | 3694 bp |
| Locus ID | 7296 |
| UniProt ID | Q16881 |
| Protein Families | Druggable Genome |
| Protein Pathways | Pyrimidine metabolism |
| Gene Summary | The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1 (also known as TXNRD1_v1). This isoform is thought to regulate expression of genes associated with differentiation and adhesion (PMID:26464515). Variants 1-3 encode the same isoform. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A direct and continuous assay for the determination of thioredoxin reductase activity in cell lysates
,null,
Analytical biochemistry
,PubMed ID 23973629
[TXNRD1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216045 | TXNRD1 (Myc-DDK-tagged)-Human thioredoxin reductase 1 (TXNRD1), transcript variant 3, (Note, selenocysteine protein, internal stop codon, see reference data summary) |
CNY 3,656.00 |
|
| RC216045L1 | Lenti-ORF, TXNRD1 (Myc-DDK-tagged)-Human thioredoxin reductase 1 (TXNRD1), transcript variant 3, (Note, selenocystein protein, internal stop codon, see summary) |
CNY 6,056.00 |
|
| RC216045L3 | Lenti-ORF, TXNRD1 (Myc-DDK-tagged)-Human thioredoxin reductase 1 (TXNRD1), transcript variant 3, (Note, selenocystein protein, internal stop codon, see summary) |
CNY 6,056.00 |
|
| RG216045 | TXNRD1 (GFP-tagged) - Human thioredoxin reductase 1 (TXNRD1), transcript variant 3, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 5,256.00 |
