HACE1 (NM_020771) Human Untagged Clone
CAT#: SC107534
HACE1 (untagged)-Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1)
CNY 6,664.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | SPPRS |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC107534 sequence for NM_020771 edited (data generated by NextGen Sequencing)
ATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACC GTGGAGTTGCCCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCT GATCAACACAGGTCTGTTTCTGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCA TTCGGACGTGTGAAAAGAAGCTTGCTTCACATTGCAGCAAATTGTGGATCGGTGGAATGC TTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATCAAGATATTTCAGGCTGTACA CCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATTATTAGAATAT AGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTG AATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAG GATGCCATGGGGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTG CAGTGCTTGCTAGACAGTGGTGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCA TTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACAGCACAGATCCTACTATTACGAGGA GCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGGATTTATGTGTACAGGGTGGA TATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCAGACTATTATT CAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTG TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACA ACAAATGGTCATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTT TTAAGGATTGTGAGAATGTTTTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAAT GGAATTGATATGGGCTACAATGGGAATAAAACTCCAAGAAGCCAGGTGTTCAAGCCTCTG GAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAATAGCCACAGAATTGATGAAA AACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGGCCAAGATCAA GATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCC ACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGAT TGTCAGGATGTTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTAC ATGTGCTGTTCTTGTCAGATGCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTC TGCAAACATGATGAAGTTTTAAAATGCTTTGTTAATAGAAATCCCAAAATTATATTTGAC CACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAAGATTCATGCATATCATAAAA GCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTCAGGACAGCCA GATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGAT TCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAA GGGATTGCTGTACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGG TTTGATATTCTGTCCAATGAGATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCT GATGGAACAACTTTTCAGCCTAATAGCAACTCTTATGTAAATCCTGATCACTTGAACTAT TTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACCACAGGCAGCTGGTCAATATT TACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTACCAAGATGTG GCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGT GATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAG GTGCCTTTGAAACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTAC GTCCAGCTTGTTACTGAACTTCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTT TTACAGGGCTTTCATATGTTCATTCCACCCTCCCTCATACAGCTTTTTGATGAATATGAA TTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGAGTGATTGGATAAAAAATACA GAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTGGGAAGTTGTA GAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGG GTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATC GCTGCTGTGCCATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTC AAGTTACCTGAATACCCAAGTAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACAT TGTGGCAGCTATGGTTACACAATGGCATAA Clone variation with respect to NM_020771.3 >OriGene 5' read for NM_020771 unedited
ATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGGGCGGCGGGGGCTGCAGGAA AGGCGCGAGCAGAGGCGGCGGCGGGTGTACTGTAGGTGGTCGGTCCGGCAGCAGCCCGGC CCCCGGACGCAGGACGTGGCCCCAGGCAGCCCTCGCAGCTCAGTGCTCTAGCCGGGGCAA GCCCGCGTCTCCGCCTGCTGGACGGGCCCAGGCGAGATGTAGGGCTCTGGGCGCGGAGGC CGCCGGTGGGGCGGCTGATCGCGGAGGATCGCGGAGGGCGCGCCGAGGATGGAGAGAGCG ATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGTTGCCC GAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCTGATCAACACAGG TCTGTTTCTGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTG AAAAGAAGCTTGCTTCACATTGCAGCAAATTGTGGATCGGTGGAATGCTTGGTTTTGCTG TTAAAGAAAGGAGCAAATCCTAACTATCAAGATATTTCAGGCTGTACACCCCTTCATTTG GCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATTATTAGAATATAGCGCTGATGTC AACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTGAATGGGCGGACA GAACTACTCCATGACCTTGTGCAGCATGTCANTGATGNNTGATGTGAGGATGCCATGGGG CAGACAGCACTGCATGTTGCCTGCCAGAACGTCACAGAAGACAGTGCAGTGCTTGCTAGA CAGTGGTGCTGATATTACAGGCCAATGTATCAGGGAGCACTCATGTACTTTGCTGCAGTC ATGTCAAAAGT |
Restriction Sites | NotI-NotI |
ACCN | NM_020771 |
Insert Size | 4000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_020771.2, NP_065822.1 |
RefSeq Size | 4614 bp |
RefSeq ORF | 2730 bp |
Locus ID | 57531 |
UniProt ID | Q8IYU2 |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (1) encodes the longest isoform (a). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205602 | HACE1 (Myc-DDK-tagged)-Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1) |
CNY 6,656.00 |
|
RC205602L1 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), Myc-DDK-tagged |
CNY 9,056.00 |
|
RC205602L2 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), mGFP tagged |
CNY 9,030.00 |
|
RC205602L3 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), Myc-DDK-tagged |
CNY 9,030.00 |
|
RC205602L4 | Lenti ORF clone of Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1), mGFP tagged |
CNY 9,056.00 |
|
RG205602 | HACE1 (tGFP-tagged) - Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1) |
CNY 8,256.00 |