NF2 (NM_181827) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACN; ACN, SCH, BANF, Merlin; BANF; merlin; moesin-ezrin-radixin like; moesin-ezrin-radizin-like protein; neurofibromin 2; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); SCH; schwannomerlin; schwannomin
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_181827, the custom clone sequence may differ by one or more nucleotides ATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACCCAAGACGTTCACCG TGAGGATCGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGATGAAGTGGAAAGGGAAGGACCTCTT TGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAACCTGGTTCTTTGGACTGCAGTACACAATCAAGGAC ACAGTGGCCTGGCTCAAAATGGACAAGAAGGTTGGGCTAGAACTCGATGAAACTGGTACTGGATCATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_181827 unedited CCCAAGTTTTTCCCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCT ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG CGGCCGCGAATCGGCACGAGGGGCGCGCGGAGTCTGGGCCGCTGCCGTCTAGGGGTCCCG TCCCGAGGCGTCCCCGGCATCTCCGGCCCGAATCCCGGAGTGCCGGGTCGCGCCTGCAGC GAAGGTCCCGGCTCCTGTGCCCTCCCTGCAGCCGTCAGGGCCCGTCCCCCAACTCCCCTT TCCGCTCAGGCAGGGTCCTCGCGGCCCATGCTGGCCGCTGGGGACCCGCGCAGCCCAGAC CGTTCCCGGGCCGGCCAGCCGGCCACCATGGTGGCCCTGAGGCCTGTGCAGCAACTCCAG GGGGGCTAAAGGGCTCAGAGTGCAGGCCGTGGGGCGCGAGGGTCCCGGGCCTGAGCCCCG CGCCATGGCCGGGGCCATCGCTTCCCGCATGAGCTTCAGCTCTCTCAAGAGGAAGCAACC CAAGACGTTCACCGTGAGGATCGTCACCATGGACGCCGAGATGGAGTTCAATTGCGAGAT GAAGTGGAAAGGGAAGGACCTCTTTGATTTGGTGTGCCGGACTCTGGGGCTCCGAGAAAC CTGGTTCTTTGGACTGCAGTACACAATCAAGGACACAGTGGCCTGGCTCACAAGGACCAA GACAGTACTGGATCATGATGCTTCAAAGGAAGAACCAGTCACCTTTCACTTCTTGGCCAA ATTNTATCCTGAGATGCTGAGAGGCACCTGGTTCCAGGAGACACACCACATTTATTCTTC TACAGGTAAGGAGCCGATTTAGATGGAAAAGACTACTGCCTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_181827 unedited GCACTGGAGGCAGGGGCTCACAGGGCATGCCACCCGGGTATCTGTTGCAGGAAAACAGCT ATGACCGCGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTAGACAAAGGAAGACATTTTCTTTTTAAAACAAAGACTCAGGTCAGAGAACTAGAACG CCATGAGATATTTCTCTACAGGGTCGTATTTCAAGGCAATTGCACATAAGAGGGGCTGGG CTCTTCACTCAGCTGGGGAAAGTTCTGGAGCTCCCACGGAAACCCCCAGCCAGCTCCCTA TGGATGGCTCTCTTGATATCTGGTCCATCCCGCGGTCCCGGTAGCAGGAGAAGTGGCAGG TCCTGGGGTGGCTGGGTCACCTGCTAGAGCTCTTCAAAGAAGGCCACTCGGGACTTGGCG CTCTGCAAGGTGAGTTTGAGGGCTCAAATGCAGATAGGTCTTCTGCCTTGGGCTTGAGGC TTTTTAATGGTATTGTGCTTGCTGCTGCCACCCCTGTCGGAGTTCTCATTGTGCAGAATA TCCAGAGCTGTCTCCCTCTCTTTCAGTTTCAAGGCCTCGATTTCTGTCTTGAGTTCATTG AGCTGCTCCTGCAGATGCTTGCTCTTTTCCATGTATTCCACTTTTTCTTTCTCTATCTCC ATGGAAAGCCGCTTCATGTCAGTATCTTTGAAGTCGAAAGACAGGCTGTCACCAATGAGG TTGAAGCTTGGTATGTCAAGAGGCAACGGTGCTGGAATTGGGTTCATGGGCGGGTACGTG GGCTTGGTGGCAATCTCCAGGAGCTTCTGCTTGGCTCTTCGCTCCGACTCGCGTGCTTCC TGGCAGGTCTGCTTAAGCTGATCTGGCTCTTT
Restriction Sites	NotI-NotI
ACCN	NM_181827
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_181827.1, NP_861965.1
RefSeq Size	6091 bp
RefSeq ORF	279 bp
Locus ID	4771
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) uses an alternate splice site in the 5' coding region that results in a frameshift, and includes an alternate segment in the 3' coding region, compared to variant 1. The resulting protein (isoform 4, also referred to as isoform insE2') is shorter than isoform 1.

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