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SKA2 (NM_182620) Human Untagged Clone
CAT＃: SC107420
SKA2 (untagged)-Human spindle and kinetochore associated complex subunit 2 (SKA2), transcript variant 1
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FAM33A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107420 sequence for NM_182620 edited (data generated by NextGen Sequencing)
ATGGAGGCGGAGGTCGATAAGCTGGAACTGATGTTCCAGAAAGCTGAGTCTGATCTGGAT TACATTCAATACAGGCTGGAATATGAAATCAAGACTAATCATCCTGATTCAGCAAGTGAG AAAAATCCAGTTACACTCTTAAAGGAATTGTCAGTGATAAAGTCTCGATATCAAACTTTG TATGCCCGCTTTAAACCAGTTGCTGTTGAGCAGAAAGAGAGTAAGAGCCGCATTTGTGCT ACTGTGAAAAAGACTATGAATATGATACAAAAACTACAGAAGCAAACAGACCTGGAGCTG TCACCACTGACTAAAGAAGAGAAAACTGCGGCAGAGCAATTCAAATTTCACATGCCAGAT TTATGA Clone variation with respect to NM_182620.3 >OriGene 5' read for NM_182620 unedited
TTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCGGAAGTCAACTATTCA ACTGGNAGCGGAGGTCGATAAGCTGGAACTGATGTTCCAGAAAGCTGAGTCTGATCTGGA TTACATTCAATACAGGCTGGAATATGAAATCAAGACTAATCATCCTGATTCAGCAAGTGA GAAAAATCCAGTTACACTCTTAAAGGAATTGTCAGTGATAAAGTCTCGATATCAAACTTT GTATGCCCGCTTTAAACCAGTTGCTGTTGAGCAGAAAGAGAGTAAGAGCCGCATTTGTGC TACTGTGAAAAAGACTATGAATATGATACAAAAACTACAGAAGCAAACAGACCTGGAGCT GTCACCACTGACTAAAGAAGAGAAAACTGCGGCAGAGCAATTCAAATCTTCACATGCCAG ATTTATGAAGAAATGGACTTGGAAAGGAAATTCTAACAGAGAAGAGCTTAATTCCGGAGA AATTTAGGAAGATGTCTTGTTAACCCTTGATGTCTAGAGATTGGGGGCTGGTGAAGGGGG TTTGGCTTCAATGACTGGATAATGATATCTTTCATGAGAGAGATTATAAGAAGAAGGGCA GATAATATATGAATAAAGTTCAGCCAAAAGGATCAAATGAGAATAAAACGATTTAAATAT ATGTACACACGCATGCACACACACACTTAGTCTTGTAATTTCAGGCCAGAAATTCTCAAC ACTATTTTGCATCTGTTTTCTTTTTCTAAGTCATGATAATATAGATGTTCTGGTCTATCA TAAAAGATGNTTATGTACATTTCATCATTCGGTATGTGGCTTTGTAAATTAAATATANGC AAAACATTGTGTTATACATGAAATAATTCATTTGGAAATGTGATNGACATGTGGTCACAT ATGTTGAGACTGCTTATGTGACN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_182620 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182620.1, NP_872426.1 |
| RefSeq Size | 1113 bp |
| RefSeq ORF | 366 bp |
| Locus ID | 348235 |
| UniProt ID | Q8WVK7 |
| Gene Summary | Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:17093495, PubMed:19289083). In the complex, it is required for SKA1 localization (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210602 | SKA2 (Myc-DDK-tagged)-Human spindle and kinetochore associated complex subunit 2 (SKA2), transcript variant 1 |
CNY 3,990.00 |
|
| RC210602L3 | Lenti-ORF clone of SKA2 (Myc-DDK-tagged)-Human spindle and kinetochore associated complex subunit 2 (SKA2), transcript variant 1 |
CNY 3,720.00 |
|
| RC210602L4 | Lenti-ORF clone of SKA2 (mGFP-tagged)-Human spindle and kinetochore associated complex subunit 2 (SKA2), transcript variant 1 |
CNY 5,890.00 |
|
| RG210602 | SKA2 (tGFP-tagged) - Human spindle and kinetochore associated complex subunit 2 (SKA2), transcript variant 1 |
CNY 2,920.00 |
