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SH3MD2 (SH3RF1) (NM_020870) Human Untagged Clone
CAT＃: SC107385
SH3RF1 (untagged)-Human SH3 domain containing ring finger 1 (SH3RF1)
CNY 6,512.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | POSH; RNF142; SH3MD2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC107385 sequence for NM_020870 edited (data generated by NextGen Sequencing)
ATGGATGAATCAGCCTTGTTGGATCTTTTGGAGTGTCCGGTGTGTCTAGAGCGCCTTGAT GCTTCTGCGAAGGTCTTGCCTTGCCAGCATACGTTTTGCAAGCGATGTTTGCTGGGGATC GTAGGTTCTCGAAATGAACTCAGATGTCCCGAGTGCAGGACTCTTGTTGGCTCGGGTGTC GAGGAGCTTCCCAGTAACATCTTGCTGGTCAGACTTCTGGATGGCATCAAACAGAGGCCT TGGAAACCTGGTCCTGGTGGGGGAAGTGGGACCAACTGCACAAATGCATTAAGGTCTCAG AGCAGCACTGTGGCTAATTGTAGCTCAAAAGATCTGCAGAGCTCCCAGGGCGGACAGCAG CCTCGGGTGCAATCCTGGAGCCCCCCAGTGAGGGGTATACCTCAGTTACCATGTGCCAAA GCATTATACAACTATGAAGGAAAAGAGCCTGGAGACCTTAAATTCAGCAAAGGTGACATC ATCATTTTGCGAAGACAAGTGGATGAAAATTGGTACCATGGGGAAGTCAATGGAATCCAT GGCTTTTTCCCCACCAACTTTGTGCAGATTATTAAACCGTTACCTCAGCCCCCACCTCAG TGCAAAGCACTTTATGACTTTGAAGTGAAAGACAAGGAAGCAGACAAAGATTGCCTTCCA TTTGCAAAGGATGATGTTCTGACTGTGATCCGAAGAGTGGATGAAAACTGGGCTGAAGGA ATGCTGGCAGACAAAATAGGAATATTTCCAATTTCATATGTTGAGTTTAACTCGGCTGCT AAGCAGCTGATAGAATGGGATAAGCCTCCTGTGCCAGGAGTTGATGCTGGAGAATGTTCC TCGGCAGCAGCCCAGAGCAGCACTGCCCCAAAGCACTCCGACACCAAGAAGAACACCAAA AAGCGGCACTCCTTCACTTCCCTCACTATGGCCAACAAGTCCTCCCAGGCATCCCAGAAC CGCCACTCCATGGAGATCAGCCCCCCTGTCCTCATCAGCTCCAGCAACCCCACTGCTGCT GCACGGATCAGCGAGCTGTCTGGGCTCTCCTGCAGTGCCCCTTCTCAGGTTCATATAAGT ACCACCGGGTTAATTGTGACCCCGCCCCCAAGCAGCCCAGTGACAACTGGCCCCTCGTTT ACTTTCCCATCAGATGTTCCCTACCAAGCTGCCCTTGGAACTTTGAATCCTCCTCTTCCA CCACCCCCTCTCCTGGCTGCCACTGTCCTTGCCTCCACACCACCAGGCGCCACCGCCGCT GCTGCTGCTGCTGGAATGGGACCGAGGCCCATGGCAGGATCCACTGACCAGATTGCACAT TTACGGCCGCAGACTCGCCCCAGTGTGTATGTTGCTATATATCCATACACTCCTCGGAAA GAGGATGAACTAGAGCTGAGAAAAGGGGAGATGTTTTTAGTGTTTGAGCGCTGCCAGGAT GGCTGGTTCAAAGGGACATCCATGCATACCAGCAAGATAGGGGTTTTCCCTGGCAATTAT GTGGCACCAGTCACAAGGGCGGTGACAAATGCTTCCCAAGCTAAAGTCCCTATGTCTACA GCTGGCCAGACAAGTCGGGGAGTGACCATGGTCAGTCCTTCCACGGCAGGAGGGCCTGCC CAGAAGCTCCAGGGAAATGGCGTGGCTGGGAGTCCCAGTGTTGTCCCCGCAGCTGTGGTA TCAGCAGCTCACATCCAGACAAGTCCTCAGGCTAAGGTCTTGTTGCACATGACGGGGCAA ATGACAGTCAACCAGGCCCGCAATGCTGTGAGGACAGNNNNAGCGCACAACCAGGAACGC CCCACGGCAGCAGTGACACCCATCCAGGTACAGAATGCCGCCGGCCTCAGCCCTGCATCT GTGGGCCTGTCCCATCACTCGCTGGCCTCCCCACAACCTGCGCCTCTGATGCCAGGCTCA GCCACGCACACTGCTGCCATCAGTATCAGTCGAGCCAGTGCCCCTCTGGCCTGTGCAGCA GCTGCTCCACTGACTTCCCCAAGCATCACCAGTGCTTCTCTGGAGGCTGAGCCCAGTGGC CGGATAGTGACCGTTCTCCCTGGACTCCCCACATCTCCTGACAGTGCTTCATCAGCTTGT GGGAACAGTTCAGCAACCAAACCAGACAAGGATAGCAAAAAAGAAAAAAAGGGTTTGTTG AAGTTGCTTTCTGGCGCCTCCACTAAACGGAAGCCCCGCGTGTCTCCTCCAGCATCGCCC ACCCTAGAAGTGGAGCTGGGCAGTGCAGAGCTTCCTCTCCAGGGAGCGGTGGGGCCCGAA CTGCCACCAGGAGGTGGCCATGGCAGGGCAGGCTCCTGCCCTGTGGACGGGGACGGACCG GTCACGACTGCAGTGGCAGGAGCAGCCCTGGCCCAGGATGCTTTTCATAGGAAGGCAAGT TCCCTGGACTCCGCAGTTCCCATCGCTCCACCTCCTCGCCAGGCCTGTTCCTCCCTGGGT CCTGTCTTGAATGAGTCTAGACCTGTCGTTTGTGAAAGGCACAGGGTGGTGGTTTCCTAT CCTCCTCAGAGTGAGGCAGAACTTGAACTTAAAGAAGGAGATATTGTGTTTGTTCATAAA AAACGAGAGGATGGCTGGTTCAAAGGCACATTACAACGTAATGGGAAAACTGGCCTTTTC CCAGGAAGCTTTGTGGAAAACATATGA Clone variation with respect to NM_020870.3 1778 t=>n;1779 t=>n;1780 g=>n;1781 c=>n >OriGene 5' read for NM_020870 unedited
GCGCGCGATTCCGGAGCGAGCGGGGGGGGCGCATCTGCGTCANCCGCCGCGGGCGCGGGG GGGCCGCGAACAAAGAGGAGGAGCCGAGGCGCGAGAGCAAAGTCTGAGATGGATGTGACA TGAGTCATTTTAAGGGGTGCGCACAACTATGAACATTTCTGAAGATTTTTTCTCAGTAAA GTAAATAAAGATGGGTGAATCANGCTTGGTGGGTCTTTTGGAGGGGCCGGGGGCGCTANA NGGCGCTTGGTGCGTTCTGCGAAGGGGTTGCGCTTGGCANCATACGTTTTCGAGGCATGT GTGGTGGGGATCGTANGGTCTCNAAATGAACTCANATGGCCCGAGGGCAGGACTCTTGGT GGCTCNGGTGGCNAGGGGCTTCCCANTAACATCTTGGTGGGCANACTTCTGGATGGGATC AAACAGGGGCCTTGGAAACCTGGTCCTGGTGGGGGAAGGNGGACCAACTGCGCAAATGCA TTAAGGGCTCANAGCAGGACTGGGGGTAATTGGANCTCAAAAATCTGCAAAGCTCCAGGG GGGACAGCAGGCTCNGGTGGCGATCTGGAGCCCCCCGTGGGGGGATACCTCAGGTACCAT GGCCAAAGCATATACAACTTGAAGGAAAGAGCCTGGAGACCTAATTGAGGGAGGGGAGCA TATCATTTTGCGAGACAGGGGGTGAAATCTGGCCCTGGGGGAGCAAGGGGATCCTGGGTT TTCCCACAACTTG >OriGene 3' read for NM_020870 unedited
CCGTAAGGCCTTGGCTGATGGCACCTTCCAGGTCCAGGATAGCACTGGGGAAGGGTCACA GGGCATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTC GAGTTTTTTTTTTTTTTTTTTTTTTTTTTCTTGAACATCAAATTTTAATCTTGAAACCTT TTATCCAGTCCTCAAATTATTAACATGAAAAGGAGTGATAAATTGCAATTTTATCATTAC CATATCACTGTGTAACAAGCCCTTGTTACAAAATCTCCCTCTACTGTCTGCAAAAAACCA ATAGAAAACCCATACCTTATATTACCTAATGATCTATTAACAGATGAAATTTTAACCAAC TTTATACCAGGAAACTATGAACAGAGGTACTTCAAATCACATAGCTTAAAATATGGAGAA AAGACAGGTAAAAAAATTATCTTAACCTGTAGTAGTCTTTTTTCTTTTTAAAATTTTTAT AAAATACACTAATTTCCCAAAATAAAGAATATTATGACACATTGGTGTCAACTTACACAG ATGAAAGCTGATGCCAGCTTTTTCCTAACTAAAGTTCATTTCACCAAACCTTTATATATT ACATGAATATCCAAGTAAAGTATTTTTTTTTTTAAATTCAACACACAGGAACATAATATA CCTTGTTTTTTTTTTAAAAAAAGGTTAATTTAAGAATGATTTCATCTTCAACCACATAAA GTATCTTTTTAAGACCATGAAACCTCTTGTCATTGCAAGACTTTTCAGTTCATGTTTCTA TTTCCCAGTTTTAATTCTTAAAGGGAAAAAAAAAAGTGAAAGGGTTTATTATGTTTTTCC ATGAAAAAACTGTGGTAAAGATCTTATTTCATTACGGGCTGTCTTCCCTCCCTTTGTTTT CCGGATACCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020870 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020870.2, NP_065921.2 |
| RefSeq Size | 5196 bp |
| RefSeq ORF | 2667 bp |
| Locus ID | 57630 |
| UniProt ID | Q7Z6J0 |
| Gene Summary | This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224916 | SH3RF1 (Myc-DDK-tagged)-Human SH3 domain containing ring finger 1 (SH3RF1) |
CNY 6,504.00 |
|
| RC224916L3 | Lenti-ORF clone of SH3RF1 (Myc-DDK-tagged)-Human SH3 domain containing ring finger 1 (SH3RF1) |
CNY 9,030.00 |
|
| RC224916L4 | Lenti-ORF clone of SH3RF1 (mGFP-tagged)-Human SH3 domain containing ring finger 1 (SH3RF1) |
CNY 9,030.00 |
|
| RG224916 | SH3RF1 (tGFP-tagged) - Human SH3 domain containing ring finger 1 (SH3RF1) |
CNY 8,104.00 |
