Cyclin B1 (CCNB1) (NM_031966) Human Untagged Clone
CAT#: SC106758
CCNB1 (untagged)-Human cyclin B1 (CCNB1)
CNY 5,488.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CCNB |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC106758 sequence for NM_031966 edited (data generated by NextGen Sequencing)
ATGGCGCTCCGAGTCACCAGGAACTCGAAAATTAATGCTGAAAATAAGGCGAAGATCAAC ATGGCAGGCGCAAAGCGCGTTCCTACGGCCCCTGCTGCAACCTCCAAGCCCGGACTGAGG CCAAGAACAGCTCTTGGGGACATTGGTAACAAAGTCAGTGAACAACTGCAGGCCAAAATG CCTATGAAGAAGGAAGCAAAACCTTCAGCTACTGGAAAAGTCATTGATAAAAAACTACCA AAACCTCTTGAAAAGGTACCTATGCTGGTGCCAGTGCCAGTGTCTGAGCCAGTGCCAGAG CCAGAACCTGAGCCAGAACCTGAGCCTGTTAAAGAAGAAAAACTTTCGCCTGAGCCTATT TTGGTTGATACTGCCTCTCCAAGCCCAATGGAAACATCTGGATGTGCCCCTGCAGAAGAA GACCTGTGTCAGGCTTTCTCTGATGTAATTCTTGCAGTAAATGATGTGGATGCAGAAGAT GGAGCTGATCCAAACCTTTGTAGTGAATATGTGAAAGATATTTATGCTTATCTGAGACAA CTTGAGGAAGAGCAAGCAGTCAGACCAAAATACCTACTGGGTCGGGAAGTCACTGGAAAC ATGAGAGCCATCCTAATTGACTGGCTAGTACAGGTTCAAATGAAATTCAGGTTGTTGCAG GAGACCATGTACATGACTGTCTCCATTATTGATCGGTTCATGCAGAATAATTGTGTGCCC AAGAAGATGCTGCAGCTGGTTGGTGTCACTGCCATGTTTATTGCAAGCAAATATGAAGAA ATGTACCCTCCAGAAATTGGTGACTTTGCTTTTGTGACTGACAACACTTATACTAAGCAC CAAATCAGACAGATGGAAATGAAGATTCTAAGAGCTTTAAACTTTGGTCTGGGTCGGCCT CTACCTTTGCACTTCCTTCGGAGAGCATCTAAGATTGGAGAGGTTGATGTCGAGCAACAT ACTTTGGCCAAATACCTGATGGAACTAACTATGTTGGACTATGACATGGTGCACTTTCCT CCTTCTCAAATTGCAGCAGGAGCTTTTTGCTTAGCACTGAAAATTCTGGATAATGGTGAA TGGACACCAACTCTACAACATTACCTGTCATATACTGAAGAATCTCTTCTTCCAGTTATG CAGCACCTGGCTAAGAATGTAGTCATGGTAAATCAAGGACTTACAAAGCACATGACTGTC AAGAACAAGTATGCCACATCGAAGCATGCTAAGATCAGCACTCTACCACAGCTGAATTCT GCACTAGTTCAAGATTTAGCCAAGGCTGTGGCAAAGGTGTAA Clone variation with respect to NM_031966.2 >OriGene 5' read for NM_031966 unedited
CAGATTTGTATACGACNACTATAGGCGGCACGCGAATTCGCACGAGTTGGTTTCTGCTGG GTGTAAGTCCTTGGCTGGTCGGGCCTCCGGTGTTCTGCTTCTCCCCGCTGAGCTGCTGCC TGGTGAATAGGAAGCCATGGCGCTCCGAGTCACCAGGAACTCGAAAATTAATGCCTGAAA ATAAGGCGAAGATCAACATGGCAGGCGCAAAGCGCGTTCCTACGGCCCCTGCTGCAACCT CCAAGCCCGGACTGAGGCCAAGAACAGCTCTTGGGGACATTGGTAACAAAGTCAGTGAAC AACTGCAGGCCAAAATGCCTATGAAGAAAGAAGCAAAACCTTCAGCTACTGGAAAAGTCA TTGATAAAAAACTACCAAAACCTCTTGAAAAGGTACCTATGCTGGTGCCAGTGCCAGTGT CTGATCCAGTGCCAGATCCAGAACCTGAGCCAGAACCTGAGCCTGTTAAAGAAGAAAAAC TTTCGCCTGAGCCTATTTTGGTTGATACTGCCTCTCCAAGCCCAATGGAAACATCTGGAT GTGCCCCTGCAGAAGAAGACCTGTGTCAAGCTTTCTCTGATGTAATTCTTGCAGTAAATG ATGTGGATGCAGAAGATGGAGCTGATCCAAACCTTTGTAGAGAATATGTGAAAGATATTT ATGCTTATCTGAGACAACTTGAGGAAGATCAAGCAGTCAGACCAAAATACCTACTGGGTC GGGAAGTCACTGGATACATGAGAGCCATCCTAATTGACTGGCTAGTACAGGTTCAAATGA AATTCAAGTTGTTGCAAGAGACCATGTACATGACTGTCTNCTTATAGATCGGTTCATGCA GAATAATTGTGTGCCCATAATAGCTGCAGCTGGNTANAGTCACTGCATGTTTATTGCATG CAATATGAGATATGTCCCTNCANAATAGAGACTTGCTTTTGTGACGACACACTGTACTAT GCACAATCAGAAGAG >OriGene 3' read for NM_031966 unedited
NGGGGAATTACTGTGNNACCGCGGCCGCTTNCTAGNGATCGGTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTCACAGATGGCACATGGGGCCAATTTTATTTGGAA ATATAGTACTCCAACTCAAGTTTACAAGTTACACCTTTGCCACAGCCTTGGCTAAATCTT GAACTAGTGCAAAATTCAGCTGGGGTAGAGGGCTGATCTTAGCATGCTTCGATGGGGCAT ACTTGTTCTTGACAGTCATGTGCTTTGTAAGTCCTTGATTTACCATGACTACATTCTTAG CCAGGTGCTGCATAACTGGAAAAAAAAATTCTTCAGTATATGACAGGTAATGTTGTAAAG TTGGTGTCCATTCACCATTATCCAGAATTTTCAGTGCTAAGCAAAAAGCTCCTGCTGCAA TTTGAGAAGGAGGAAAGTGCACCATGTCATAGTCCAACATAGTTAGTTCCATCAGGTATT TGGCCAAAGTATGTTGCTCGACATCAACCTCTCCAATCTTAGATGCTCTCCGAAGGAAGT GCAAAGGTAGAGGCCGACCCAGACCAAAGTTTAAAGCTCTTAGAATCTTCATTTCCATCT GTCTGATTTGGTGCTTAGTATAAGTGTTGGCAGTCACAAAAGCAAAGTCACCAATTTCTG GAGGGTACATTTCTTCAATATTGCTTGGCATAAACATGGCAGTGACACCCAACAGCTGCA GCATCTTTTTGGGCACACAATTATTCTGGATGAACCGATCAATAATGGAGAACAGTCTGT ACATGGTCTCCTGCAACAACCTGGAATTTCATTTTGACCCTGGCCTAGCCAGTCCATTAA GATGGCTCTCATGGTTCCAGGGCCTTCCGGACCCAAAGGGATTTTGGCCTGACGG |
Restriction Sites | NotI-NotI |
ACCN | NM_031966 |
Insert Size | 1500 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031966.2, NP_114172.1 |
RefSeq Size | 2101 bp |
RefSeq ORF | 1302 bp |
Locus ID | 891 |
UniProt ID | P14635 |
Domains | cyclin_C, CYCLIN, cyclin |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation |
Gene Summary | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200812 | CCNB1 (Myc-DDK-tagged)-Human cyclin B1 (CCNB1) |
CNY 5,856.00 |
|
RC200812L1 | Lenti ORF clone of Human cyclin B1 (CCNB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200812L2 | Lenti ORF clone of Human cyclin B1 (CCNB1), mGFP tagged |
CNY 5,890.00 |
|
RC200812L3 | Lenti ORF clone of Human cyclin B1 (CCNB1), Myc-DDK-tagged |
CNY 8,256.00 |
|
RC200812L4 | Lenti ORF clone of Human cyclin B1 (CCNB1), mGFP tagged |
CNY 8,256.00 |
|
RG200812 | CCNB1 (tGFP-tagged) - Human cyclin B1 (CCNB1) |
CNY 7,456.00 |
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