UBE2J2 (AF296658) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NCUBE-2; NCUBE2; PRO2121
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for AF296658 edited GCGGCGGCGGCGGCGGCGCTGCGGCGGGTTCGGTGGGCCCAATCCCGGGGCGGTGCGGCT GTTTCGGGCGCGGGCCCCGCTTTTCCGCACCCTGCTCCGGCCTCGACTACGGCGAGCCTG AGCGCGGCGGCGGCCCACGCGCAGCGACAGGGAGAGATGAGCAGCACCAGCAGTAAGAGG GCTCCGACCACGGCAACCCAGAGGCTGAAGCAGGACTACCTTCGCATTAAGAAAGACCCG GTGCCTTACATCTGTGCCGAGCCCCTCCCTTCGAATATTCTCGAGTGGCACTATGTCGTC CGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAACTAATTTTTCCC AGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCACTCCCAACGGGAGGTTTAAG TGCAACACCAGGCTGTGTCTTTCTATCACGGATTTCCACCCGGACACGTGGAACCCGGCC TGGTCTGTCTCCACCATCCTGACTGGGCTCCTGAGCTTCATGGTGGAGAAGGGCCCCACC CTGGGCAGTATAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTGCAGAGTTTAGCA TTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTGGAGGAGATTAAA CAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCCAGACTCTCCCCTTGCCAGAC GTGGTTCCAGACGGGGAGACGCACCTCGTCCAGAACGGGATTCAGCTGCTCAACGGGCAT GCGCCGGGGGCCGTCCCAAACCTCGCAGGGCTCCAGCAGGCCAACCGGCACCACGGACTC CTGGGTGGCGCCCTGGCGAACTTGTTTGTGATAGTTGGGTTTGCAGCCTTTGCTTACACG GTCAAGTACGTGCTGAGGAGCATCGCGCAGGAGTGAGGCCCAGGCGCCGAGACCCAAGGC GCCACTGAGGGCACCGCGCACCAGAGCGTGACCTCGGCAGGCTGGACACACTGCCCAGCA CAGGCAGACCCACC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF296658 unedited CCCCCCAGGAGACCCCAAAACCCCCCCCCCCNCCCCCCTTTGCGGCAATTTGAATACGTT TCACTATAGGGCGGCCGCGCAATCGGCACCAGNAGGCGGCTTTTGCGGCGGCGCTGCGGG GGGGTCGGTGGGCCCAATCCCGGGGCGGTGCGGCTGTTTCGGGCGCGGGCCCCGCTTTTC CGCACCCTGCTCCGGCCTCGACTACGGCGAGCCTGAGCGCGGCGGCGGCCCACGCGCAGC GACAGGGAGAGATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCAGAGGC TGAAGCAGGACTACCTTCGCATTAAGAAAGACCCGGTGCCTTACATCTGTGCCGAGCCCC TCCCTTCGAATATTCTCGAGTGGCACTATGTCGTCCGAGGCCCAGAGATGACCCCTTATG AAGGTGGCTATTATCATGGAAAACTAATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCA GTATCTATATGATCACTCCCAACGGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTA TCACGGATTTCCACCCGGACACGTGGAACCCGGCCTGGTCTGTCTCCACCATCCTGACTG GGCTCCTGAGCTTAATGGTGGAGAAGGGCCCCACCCTGGGCAGTATAGAGACGTCCGACT TCACGAAAAGACAACTGGAAGTGCACAGTTTACACTTTAATTTGAAAGATAAAGTCTTTT GTGAACCATCCCCTGAAGCCCGCGCGGGAGATTAAACACAACCAGAAAGCCCCAGGACGA ACCCCGTAGCCGACACCAGAACTCTCCCCTTGCACACCGGGTTCCCGACGGGGAGAACGA ACTTGTCCAGAACGGGATCAAACTGCTCAACTGGCCTGCGCCCGGGGGCCGCCCCAAACC TCCAAGGCCTCCACAGGGCAACCGGACCACGGACT
Restriction Sites	NotI-NotI
ACCN	AF296658
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF296658.1, AAK52609.1
RefSeq Size	1238 bp
RefSeq ORF	777 bp
Locus ID	118424
Domains	UBCc
Protein Families	Transmembrane
Protein Pathways	Parkinson's disease, Ubiquitin mediated proteolysis
Gene Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

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