PRMT3 (AF059531) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HRMT1L3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for AF059531 edited ATGTGCTCGTTAGCGTCAGGCGCTACCGGCGGCCGGGGCGCTGTGGAGAATGAGGAGGAC CTGCCAGAACTGTCGGACAGCGGGGACGAGGCCGCCTGGGAGGATGAGGACGATGCAGAT CTCCCCCACGGCAAGCAGCAGACCCCCTGCCTGTTCTGTAACAGGTTATTCACATCTGCT GAAGAAACATTTTCACACTGTAAGTCTGAGCATCAGTTTAATATTGACAGCATGGTTCAT AAACATGGACTTGAATTTTATGGATACATTAAGCTAATAAATTTTATTAGACTTAAGAAT CCTACAGTTGAGTACATGAATTCCATATACAACCCAGTGCCTTGGGAGAAAGAAGAGTAT TTGAAGCCAGTATTAGAAGATGACCTTTTACTTCAATTTGATGTAGAAGATCTTTATGAA CCGGTGTCAGTACCCTTCTCATACCCCAATGGACTCAGTGAAAATACATCTGTTGTTGAA AAATTGAAACATATGGAAGCCAGGGCACTGTCTGCTGAAGCCGCATTGGCCAGAGCACGT GAGGATCTGCAAAAAATGAAACAATTTGCTCAGGATTTTGTGATGCACACAGATGTCAGA ACCTGCTCGTCATCTACTAGTGTCATTGCGGACCTCCAGGAGGATGAGGATGGTGTTTAT TTCAGCTCATACGGGCATTATGGGATACATGAAGAAATGCTAAAGGACAAAATACGAACA GAAAGCTACCGAGATTTCATATACCAAAATCCACATATCTTCAAAGACAAGGTAGTTTTG GATGTTGGGTGTGGAACTGGAATTCTCTCTATGTTTGCTGCTAAAGCTGGGGCGAAGAAG GTTCTTGGAGTTGATCAATCTGAAATACTTTACCAGGCAATGGATATTATAAGACTAAAT AAACTTGAAGATACTATTACACTAATTAAAGGAAAGATTGAAGAAGTTCATCTTCCTGTA GAAAAAGTAGATGTTATCATATCTGAGTGGATGGGCTATTTTCTTCTGTTTGAGTCTATG TTAGATTCTGTCCTTTATGCAAAGAACAAATACTTGGCAAAAGGAGGCTCGGTCTACCCT GACATTTGCACTATCAGCCTTGTAGCAGTGAGTGATGTGAATAAACATGCTGATAGAATT GCTTTTTGGGATGATGTCTATGGCTTCAAGATGTCCTGCATGAAGAAAGCAGTTATTCCA GAAGCTGTTGTGGAAGTTTTAGATCCGAAGACTCTTATTTCAGAACCTTGTGGTATTAAG CATATAGATTGCCATACGACGTCTATCTCAGATTTGGAATTTTCATCAGATTTTACCGTG AAAATCACAAGGACATCCATGTGCACGGCAATTGCTGGCTACTTTGATATATATTTTGAG AAGAATTGCCACAACAGGGTCGTGTTCTCTACGGGCCCTCAGAGCACCAAAACACACTGG AAACAAACAGTATTTCTACTGGAAAAACCATTTTCAGTTAAAGCAGGTGAAGCCTTGAAA GGAAAGGTCACAGTTCACAAGAATAAGAAAGATCCACGTTCTCTCACCGTGACCCTCACG TTGAATAATTCAACTCAAACTTATGGTCTCCAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF059531 unedited CGCGCCGGGCGTNAGCAGATANTTATCCCCCCCGGGGTTGAGGATTTGTAATACNACTAC TATAGGCGGCCGCGCATTCGGCACGACCACACCTGTGCTCGTTAGCGTCAGGCGCTACCG GCGGCCGGGGCGCTGTGGAGAATGAGGAGGACCTGCCAGAACTGTCGGACAGCGGGGACG AGGCCGCCTGGGAGGATGAGGACGATGCAGATCTCCCCCACGGCAAGCAGCAGACCCCCT GCCTGTTCTGTAACAGGTTATTCACATCTGCTGAAGAAACATTTTCACACTGTAAGTCTG AGCATCAGTTTAATATTGACAGCATGGTTCATAAACATGGACTTGAATTTTATGGATACA TTAAGCTAATAAATTTTATTAGACTTAAGAATCCTACAGTTGAGTACATGAATTCCATAT ACAACCCAGTGCCTTGGGAGAAAGAAGAGTATTTGAAGCCAGTATTAGAAGATGACCTTT TACTTCAATTTGATGTAGAAGATCTTTATGAACCGGTGTCAGTACCCTTCTCATACCCCA ATGGACTCAGTGAAAATACATCTGTTGTTGAAAAATTGAAACATATGGAAGCCAGGGCAC TGTCTGCTGAAGCCGCATTGGCCAGAGCACGTGAGGATCTGCAAAAAATGAAACAATNTG CTCAGGATTTTGTGATGCACACAGATGTCAGAACCTGCTCGTCATCTACTAGTGTCATTG CGGACCTNNCAGGAGATGANGATGGTGTTTATTTCAGCTCATACGGGCATTATGGGATAC ATGANGAAATGCTAAAGGACANAATACGAACAGAAAGCTACCGAGATTTCATATACCANA ATCCACATATCTTCAAAGACANGGTAGTTNTGGATGTTGGGTGTGGAACTGGNAATCTCT CTATGTTTGCTGCTTAAGCTGGGGCGAAAAAAGTTCCTGGNAGTTGATCAATCTGAATAC TTTACCCAGCATGATATTTATAGACTAAATAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF059531 unedited NAAGGTACACTATGNACGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTACAT TTTATTAAAATTTTATTCTACATTTCAAATTGAATCAGTAATTGTAAATGACAACTTTAT AGACTGCTTAGTAACAGTTACATGTTTAACCTTTGCACATAAATCTGGTTTGAAAAAGAT AATACGTTAGTAAATAAGTACATATTTATCCCAATCTTTAAAAACTTTCATGAAACCTAG GATAAATGTATTGAAATTAGATTTCCTGGGCAGTACTAGAAATCTATAAGGCTAAGGAAT AGTCACAAAGAGGGAAGAAACTACAAAATGAGAACCCTAAGTATGCTAGATAATCAACTA GGCTGTCTGCATATATGATTTGTATATGAAGGCTGAAGCTCTTTTCCATATTTTAGTTTT TTTATACAAATCTTTATTACATCCTGATTTTCTAGCAAATACAATTAATAACTCCTCCTC AGAGGAGTCCATAATAAAATGTGTAGTACCTGTGTTACTAGTTTAATAAAATATCCAAAT AACTAACCAATTTGTTTAAAATTTATAGTAAGAAACTTATTAAAAACAAAAGACTATCTC TCTTCCAAATATAGGCCAATGTAAATGATTTTCAATATAGATAAACTCAATGTGGGAGTT ACATTTTGGTTAAATCTGGTGGAGCAAACGTCTAAGAAATGATTACGTGGCAGACCATTG AGATCAGCTGATTCTCTTCCTCAGCTGAACTATGTCAGATTCCCACAATGAGGACTTCTC TCTTATTGAGGAGCTCATTTAGAAAGGGTCATAATCCATCCTTCTGCTCACATAAAACAG CTCCCTCTGCTGACC
Restriction Sites	NotI-NotI
ACCN	AF059531
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF059531.1, AAC39837.1
RefSeq Size	2442 bp
RefSeq ORF	2442 bp
Locus ID	10196
Protein Families	Druggable Genome
Gene Summary	This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

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