UXS 1 (UXS1) (BC009819) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SDR6E1; UGD
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106349 sequence for BC009819 edited (data generated by NextGen Sequencing) ATGGTGAGCAAGGCGCTGCTGCGCCTCGTGTCTGCCGTCAACCGCAGGAGGATGAAGCTG CTGCTGGGCATCGCCTTGCTGGCCTACGTCGCCTCTGTTTGGGGCAACTTCGTTAATATG AGGTCTATCCAGGAAAATGGTGAACTAAAAATTGAAAGCAAGATTGAAGAGATGGTTGAA CCACTAAGAGAGAAAATCAGAGATTTAGAAAAAAGCTTTACCCAGAAATACCCACCAGTA AAGTTTTTATCAGAAAAGGATCGGAAAAGAATTTTGATAACAGGAGGCGCAGGGTTCGTG GGCTCCCATCTAACTGACAAACTCATGATGGACGGCCACGAGGTGACCGTGGTGGACAAT TTCTTCACGGGCAGGAAGAGAAACGTGGAGCACTGGATCGGACATGAGAACTTCGAGTTG ATTAACCACGACGTGGTGGAGCCCCTCTACATCGAGGTTGACCAGATATACCATCTGGCA TCTCCAGCCTCCCCTCCAAACTACATGTATAATCCTATCAAGACATTAAAGACCAATACG ATTGGGACATTAAACATGTTGGGGCTGGCAAAACGAGTCGGTGCCCGTCTGCTCCTGGCC TCCACATCGGAGGTGTATGGAGATCCTGAAGTCCACCCTCAAAGTGAGGATTACTGGGGC CACGTGAATCCAATAGGACCTCGGGCCTGCTACGATGAAGGCAAACGTGTTGCAGAGACC ATGTGCTATGCCTACATGAAGCAGGAAGGCGTGGAAGTGCGAGTGGCCAGAATCTTCAAC ACCTTTGGGCCACGCATGCACATGAACGATGGGCGAGTAGTCAGCAACTTCATCCTGCAG GCGCTCCAGGGGGAGCCACTCACGGTATACGGATCCGGGTCTCAGACAAGGGCGTTCCAG TACGTCAGCGATCTAGTGAATGGCCTCGTGGCTCTCATGAACAGCAACGTCAGCAGCCCG GTCAACCTGGGGAACCCAGAAGAACACACAATCCTAGAATTTGCTCAGTTAATTAAAAAC CTTGTTGGTAGCGGAAGTGAAATTCAGTTTCTCTCCGAAGCCCAGGATGACCCACAGAAA AGAAAACCAGACATCAAAAAAGCAAAGCTGATGCTGGGGTGGGAGCCCGTGGTCCCGCTG GAGGAAGGTTTAAACAAAGCAATTCACTACTTCCGTAAAGAACTCGAGTACCAGGCAAAT AATCAGTACATCCCCAAACCAAAGCCTGCCAGAATAAAGAAAGGACGGACTCGCCACAGC TGA Clone variation with respect to BC009819.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009819 unedited TCAGGATTTTGTACTACGATTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGCGGG CCCCCGCGCGGCAGGGCCCTGGACCCGCGCGGCTCCCGGGGATGGTGAGCAAGGCGCTGC TGCGCCTCGTGTCTGCCGTCAACCGCAGGAGGATGAAGCTGCTGCTGGGCATCGCCTTGC TGGCCTACGTCGCCTCTGTTTGGGGCAACTTCGTTAATATGAGGTCTATCCAGGAAAATG GTGAACTAAAAATTGAAAGCAAGATTGAAGAGATGGTTGAACCACTAAGAGAGAAAATCA GAGATTTAGAAAAAAGCTTTACCCAGAAATACCCACCAGTAAAGTTTTTATCAGAAAAGG ATCGGAAAAGAATTTTGATAACAGGAGGCGCAGGGTTCGTGGGCTCCCATCTAACTGACA AACTCATGATGGACGGCCACGAGGTGACCGTGGTGGACAATTTCTTCACGGGCAGGAAGA GAAACGTGGAGCACTGGATCGGACATGAGAACTTCGAGTTGATTAACCACGACGTGGTGG AGCCCCTCTACATCGAGGTTGACCAGATATACCATCTGGCATCTCCAGCCTCCCCTCCAA ACTACATGTATAATCCTATCAAGACATTAAAGACCAATACGATTGGGACATTAAACATGT TGGGGCTGGCAAAACGAGTCGGTGCCCGTCTGCTCCTGGCCTCCACATCGGAGGTGTATG GAGATCCTGCAGTCCACCCTCAAAGTGAGGATTACTGTNGCCACGTGAATCCAATAGGAC CCTGGGCCTGCTACCATGAAAGCAAACGTGTTGCANAAACCATGTGCTATGCCTACATGA CCCAAGAACGCGTGGAAGTGCCAGTGCCCANAATCTTCAACACCTTTGGCCCACGCTGCA CATGACCGATGGCGAGTAGTCACAACTCCTTCTGCAGCGCTCCAGGGAAGCACTCACGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC009819 unedited NACTATGTACGCGGCCGCTTTCTANAGTCGAGTTATATCTTTTTTTTTTTTTTCATAGTA TAACGACTCATTTATTAAGTACCCACTTTTATAAATAAAATCGGCATTTTTTGTCCCGCT TAATGTTCCTTTTCAGCTTCACAAAGAAACCAGTAAATAAAACTGTCAACGACAGTCACA ACATATGCTCTCACAGCAAGATAAAAAAACTTGAAAATACGCAGAGATGCATCTACGCTA TTTTACATAAAAAGAGAGATTCAAAAAGTGCAAGGCAAAATCTGCAGTTTTTTGAGGGGA GCTTTTAGGCACATCCATTTCATTAAAGCAAGCTTCAGAATGAAATTCCAGTTTGTTCTT CATGACACCTGTTAAAGTCTTTCTTTAAACGACAACAAAAAAAAGCCAAAAATACATCCC ATCAAGTGTACAATGGTAGTCTTGTGTCCTAAAAGTGAGGAGTTCAGCTGTGGCGAGTCC GTCCTTTCTTTATTCTGGCAGGCTTTGGTTTGGGGATGTACTGATTATTTGCCTGGTACT CGAGTTCTTTACGGAAGTAGTGAATTGCTTTGTTTAAACCTTCCTCCAGCGGGACCACGG GCTCCCACCCCAGCATCAGCTTTGCTTTTTTGATGTCTGGGTTTCTTTTCTGTGGGTCAT CCTGGGCTTCGGAGAGAAACTGAATTTCACTTCCGCTACCAACAAAGTTTTTAATTAACT GAGCAAATTCTAGGGATGTGTGTTCTTCTGGGTTCCCCAGGTTGACCGGGCTGCTGACGT TGCTGTTCATGAAAGCACGAGGCCATTCACTAGATCGCTGACGTACTGGAACGCCCCTGT CTGAAACCCGGATCGTATACCGTGAGTGGCTCCCCTGAAGCGCTGCAGGATGAAATGCTG CTACTCCCCATCGTAATGTGCAGCGCGGCCAAAGG
Restriction Sites	NotI-NotI
ACCN	BC009819
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009819.2, AAH09819.2
RefSeq Size	2031 bp
RefSeq ORF	1263 bp
Locus ID	80146
Domains	Epimerase
Protein Families	Transmembrane
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Metabolic pathways, Starch and sucrose metabolism
Gene Summary	This gene encodes an enzyme found in the perinuclear Golgi which catalyzes the synthesis of UDP-xylose used in glycosaminoglycan (GAG) synthesis on proteoglycans. The GAG chains are covalently attached to proteoglycans which participate in signaling pathways during development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

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