PCCA (AF385926) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF385926, the custom clone sequence may differ by one or more nucleotides ATGGCGGGGTTCTGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGC AGCTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGCTTAATGGT GTCCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGATAAAATTCTTGTTGCTAAT AGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGCATTAAGACAGTTGCCATCCACA GTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCAC CAGTAAAAGCTACCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACAT CCAGGTTATGGATTCCTTTCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCA TTGGACCTGACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAAGC AGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTCAGAATTGCAAGG GAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCAGGTGGTGGTGGGAAAGGCATGCGCATTGCTTGGG ATGATGAAGAGACCAGGGATGGTTTTAGATTGTCATCTCAAGAAGCTGCTTCTAGTTTTGGCGATGATAG ACTACTAATAGAAAAATTTATTGATAATCCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGG AATGCTTTATGGCTTAATGAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTGGAGGAAGCAC CAAGCATTTTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCTGTAGCTCTTGCCAGAGCAGT AAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGACTCTAAGAAGAATTTTTATTTCTTGGAAATG AATACAAGACTCCAGGTTGAGCATCCTGTCACAGAATGCATTACTGGCCTGGACCTAGTCCAGGAAATGA TCCGTGTTGCTAAGGGCTACCCTCTCAGGCACAAACAAGCTGATATTCGCATCAACGGCTGGGCAGTTGA ATGTCGGGTTTATGCTGAGGACCCCTACAAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTAC CAAGAACCGTTACATCTACCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGATATTAGCATTT ATTATGATCCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACTGAGGCACTGAAGAGAATGGC AGATGCACTGGATAACTATGTTATTCGAGGTGTTACACATAATATTGCATTACTTCGAGAGGTGATAATC AACTCACGCTTTGTAAAAGGAGACATCAGCACTAAATTTCTCTCCGATGTGTATCCTGATGGCTTCAAAG GACACATGCTAACCAAGAGTGAGAAGAACCAGTTATTGGCAATAGCATCATCATTGTTTGTGGCATTCCA GTTAAGAGCACAACATTTTCAAGAAAATTCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAG CTCTCAGTAAAATTGCATGATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTGTTCTCGGTGG AAGTTGATGGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCGCCCTTATTGTCTGTCAGCGT TGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAAGCAGGTGGAAACATGAGCATTCAGTTTCTT GGTACAGTGTACAAGGTGAATATCTTAACCAGACTTGCCGCAGAATTGAACAAATTTATGCTGGAAAAAG TGACTGAGGACACAAGCAGTGTTCTGCGTTCCCCGATGCCCGGAGTGGTGGTGGCCGTCTCTGTCAAGCC TGGAGACGCGGTAGCAGAAGGTCAAGAAATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACA GCTGGGAAAACTGGCACGGTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAAGGGGATCTGC TCGTGGAGCTGGAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF385926 unedited GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGC ACGAGGGGTCAGCAGAGGGGCGGTCTGCGGGGACAACAATGGCGGGGTTCTGGGTCGGGA CAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGGCCGCCGCAGCAGCTGATGCTGA GCGCGGCGCTGCGGACCCTGAAGCATGTTCTGTACTATTCAAGACAGTGCTTAATGGTGT CCCGTAATCTTGGTTCAGTGGGATATGATCCTAATGAAAAAACTTTTGATAAAATTCTTG TTGCTAATAGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGCATTA AGACAGTTGCCATCCACAGTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCGGATG AGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCTCAACATGGATGCCATCA TGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACATCCAGGTTATGGATTCCTTTCAG AAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCATTGGACCTGACA CACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAAGCAG AGGTTAATACAATCCCTGGCTNTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTCAGAA TTGCAAGGGAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCANGTGGTGGTGGGGAAA GCATGCGCATTGCTTGGGATGATGAAAAGACAGNGNATGGTTNNTAGATTGTCATCTCAA GAAAGCTGCTTCTAGTTTTGGGCGATGATAGACTACTAATAGAANAAATTATTGATNNAT CCTCGCATATAGAAATCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF385926 unedited CGCAATCTAGAGTCGAGTNNTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTAAACAAA TGCTCAGTTTTATTGATTTAATTTTGACACTAGGGAATCTCACAGCAGAAGTACAAATCA TATGTACAAGTATTTATATCAATGAAAATTTCCATTGGTGATTTTTTGGCAAAATATTGG TCTTGACTCTGTGGAATAAATGACGACGTAAACGTAGCTGCACAGGGGTGTTCCTGTATA ATGCTTGAATCAATTGTGTGTGAAAGCATCATGCAAATGGCTAATTAAATTGGGTGATGA CTGAAAGGTTATAAATCCTTCATTCCAGCTCCACGAGCAGATCCCCTTCTCCAACTGTGT CTCCAGCTTGACAGTGCACAGATTTCACCGTGCCAGTTTTCCCAGCTGTCATACTATTCT GCATTTTCATGGCTTCAATCACACAAATTTCTTGACCTTCTGCTACCGCGTCTCCAGGCT TGACAGATACGGCCACCACCACTCCGGGCATCGGGGAACGCAGAACACTGCTTGTGTCCT CAGTCACTTTTTCCAGCATAAATTTGTTCAATTCTGCGGCAAGTCTGGTTAAGATATTCA CCTTGTACACTGTACCAAGAAACTGAATGCTCATGTTTCCACCTGCTTCTCGAGAAAGAC ACTGGACAGTCCTCTGAGTGCCATCAACGCTGACAGACAATAAGGGCGAAAGCCAGTTCC ACGTGCTGGTCACATTTAGTTTCGACCCATCAACTTCCACGAGAACACTGACCCATTGTT TGATGCTACTACGGTATGAACTTTATTATGCAATTTTACTGAAAGCTCCCATTGGCTATG TCTGGTTTAATACAAGCATCCTTGATTTTCTTGAAAAGTTGGGCCTCTTACTGGATGCCC CAACATGAGGAGCCTTGCCAAAACTGGTCTCTNACTCTTGGTAACAGGGGGCTTGGACCT CCAGATCCCTCGGAAAAATTTAGGCGAGTGCCTTTAAAACGGGG
Restriction Sites	NotI-NotI
ACCN	AF385926
Insert Size	2620 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF385926.1, AAL66189.1
RefSeq Size	2488 bp
RefSeq ORF	2187 bp
Locus ID	5095
Domains	biotin_lipoyl, CPSase_L_D2, CPSase_L_chain, Biotin_carb_C
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
Gene Summary	The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.