PHPT1 (AF131857) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGI-202; HEL-S-132P; HSPC141; PHP14
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF131857, the custom clone sequence may differ by one or more nucleotides GGCCTCCCTCGTTGCCCCAGCCTCGCGGGCCGCCTAACTGCCCCGTTCCAAGGGTGCCACCGGACCCCGC TGGAGAGGAACTTCTCCGTTGGCTGATTTCATCACCACCCATTCCCGATTCCACGTTTCCTTTAAGCGGG GCTGGCGGAGCCGCAAGGCGGCAAGGAACTGGATTGCGATTGGTCAGCACGTGCCTCGGTCGGCGGTACA ATTGGCTGAGGCGCTGGGCCTTGGGAAGCATTCCCCGACGGGATTGGTCGTCGCTCTCGCAGAGCCCGCC TCCCGCAGTACAAGCGGCCCCCGGGTCGGGTGGGAGGAGGGGACTCCGGGAGGAGGAACATGGCGGTGGC GGACCTCGCTCTCATTCCTGATGTGGACATCGACTCCGACGGCGTCTTCAAGTATGTGCTGATCCGAGTC CACTCGGCTCCCCGCTCCGGGGCTCCGGCTGCAGAGAGCAAGGAGATCGTGCGCGGCTACAAGTGGGCTG AGTACCATGCGGACATCTACGACAAAGTGTCGGGCGACATGCAGAAGCAAGGCTGCGACTGTGAGTGTCT GGGCGGCGGGCGCATCTCCCACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTATTCCATGGTGAGC CGCAGCCCCGTCCCGCCCTGCCGGAGGCCCCAGTACCAGCTTCGAGGCCCACCTGAGCCTGCTGCCCTGA CCCGTGGCCCCAGCTGAGCACGCAGGCTTCCTGGGGTTCTCCCAGGGTCGGCGGCAGAGCCCTCCCTCCA GGGCCCATTGTGTTCCTGCATTCCCCCATGGAGCACACGCCAGACCTGAGGGGTGGGACGGACACCCCCA GGCATGGCCGGCTGTCTCCTCTCCCTGCCTTGGGAGGCCTTGCTGGGCTCTAGCTGTCCTCCAGCACTTT GGGCCCTGGGCCCCCAGAGGCAGTCAGTACCTGGGTGGAGCTCAGAGTCCCCACCTGTGCTCTTCACAAA AACCACCAGCAGATGAGACCCACGTGCGTCCCTCTGGGCGCCTCAGGCCCCAGGATCCACCATCAAGGCC TATGGTCCTGCCCAGCACGCCATTTCAACTGAGAAAATCAAAGCCAAGTACCCCGACTACGAGGTCACCT GGGCTAACGACGGCTACTGAGCACTCCCAGCCCGGGGCCTGCTGCCTCCAGCAGCCACTTCAGAGCCCCC GCCTTTGCCTGCACTCCTCTTGCAGGGCTGGCCCTGCCTGCTCCTGCGGCAGCCTCTGGTGACGTGCTGT CCACCAGGCCTTGGAGACAGGCTAGCCTGGCCACAGAATTAAACGTGTTGCCACACCAAAAAAAAAAAAA AAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF131857 unedited GTAACTGGCAACACCCTACCCTTCCCCNNCGCGGTTCGGATTTGTAAACGACTCACTATA GGGCGGCCGCGAATCGGCACCAGGGCAAGTCCCTCAGGCCTCCCTCGTTGCCCCAGCCTC GCGGGCCGCCTAACTGCCCCGTTCCAAGGGTGCCACCGGACCCCGCTGGAGAGGAACTTC TCCGTTGGCTGATTTCATCACCACCCATTCCCGATTCCACGTTTCCTTTAAGCGGGGCTG GCGGAGCCGCAAGGCGGCAAGGAACTGGATTGCGATTGGTCAGCACGTGCCTCGGTCGGC GGTACAATTGGCTGAGGCGCTGGGCCTTGGGAAGCATTCCCCGACGGGATTGGTCGTCGC TCTCGCAGAGCCCGCCTCCCGCAGTACAAGCGGCCCCCGGGTCGGGTGGGAGGAGGGGAC TCCGGGAGGAGGAACATGGCGGTGGCGGACCTCGCTCTCATTCCTGATGTGGACATCGAC TCCGACGGCGTCTTCAAGTATGTGCTGATCCGAGTCCACTCGGCTCCCCGCTCCGGGGCT CCGGCTGCAGAGAGCAAGGAGATCGTGCGCGGCTACAAGTGGGCTGAGTACCATGCGGAC ATCTACGACAAAGTGTCGGGCGACATGCAGAAGCAAGGCTGCGACTGTGAGTGTCTGGGC GGCGGGCGCATCTCCCACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTATTCCATG GTGAGCCGCAGCCCCGTCCCGCCCTGCCCNAGGCCCCAGTACCAGCTTCGAGGCCCACCT GAGCCTGCTGCCCTGACCCGTGGCCCCAGCTGAGCACGCANGCTTTCTGNNNGTTCTCCA GGNTCCGCGGCAGAGCCCTCCCTCCCAGGCCCAATGTGTTTCCGCCTTCCCCCAGGNAGC ACACCCCANACCTGAGGGGTGGGGACCGGACACCTCCAAGNCATGGCCCGGCTGTTCTT
Restriction Sites	NotI-NotI
ACCN	AF131857
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF131857.1
RefSeq Size	1338 bp
RefSeq ORF	1338 bp
Locus ID	29085
Domains	Ocnus
Protein Families	Druggable Genome
Protein Pathways	Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
Gene Summary	This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.