IBRDC2 (RNF144B) (AJ420555) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA528A10.3; IBRDC2; p53RFP; PIR2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AJ420555, the custom clone sequence may differ by one or more nucleotides GGCACGAGGCGGCACGAGGTCTGTCAGTAAACTCTTTAAGCTTGGTGCCGCAAAGAGTCTTTAAATGGGG GCTGATTTCAAGTAACCTAAAAGACTGTGTTATCAGAGGAAGAGGTCCCAAATTTGGAGTAAAGATGGGA GAAAATAAATATGTGCTATTTCCTTGGCGAGTTGGGGGAATTTGCCACCTTACAGAGTTTGTATCACTGA ATTAGCTGCTTTTGTTTTTTTTTTTTTTTTTTTTTTGCCAGGGCTATGGAGTGGGGGTTGTTTGTCAAAC TGATTTTCAATAATTGGATTTAATTTTTTTTAACATTGAAAAGTGCCTGAAAAATGGTAAATTCTTAAAT GTGTGTGAGATTGTCAGAATCAACAAAACTAGGTTGGTTAAACATATCTCTGGTACATCAAGGGGCATGA TACAAACCAGTCTAAAGACTGTTTATAAAGGAGAGAGCTGGCGACTTATTTTTATTTTTTTTTTTTGGAC AGAGTCTCCCTTTGTCACCCAGGCCGGAGTGCAGTGGCATGATCATGGTTCACTTCAACCCCTACCTCCT GGGCTCAAGTGTTCCTCTCACCTTATCCTCCTGAGCAGCTGGGACTACAGGCACACACCACCACACCTGG CTAAGTTTTGTATTTTTTGTAGAGATGGGGTTTCTCTGTGTTGCCCAGTCTTGTCTCAAACTCCTGGGCT CAAGCGATCCACCCACCTTGGGCTCCCAAAGTGTTGGGATTACAGGTGTGTGTCACTGTGCCTGACAGCT GACAGTTTTAACTGACAACTTTGATAACAGAGGCTGCTATTTTTGTTTTAGATAATTGGCCAGTGACAGA GTTTACCCTTGCCTCCTTTCTTGGTCTGCCAGCTTTGTCCTTTCTGAGTGATTCTCTTTCTGTATTGAGA GGAAGTGTGGGTCTACATAGGGATGTTTGGATGCTATGGCAAGAATCTTTTTGTGTTTGGAGTGTAGTCC ATTTGCAATAGAAATAAAAAAATCCGTCACCAAATTGTAACCTGGATGTTATAGCCCAGCATCTAGAAAT CCTATGAAATGTATTAGCACAATATCTTGCCATTGTCCCATCTAGGAAATTTTTTCTTGTTGTGAGGTAG GGAAGTGAGGAGGAAAGCCATGCCGAAGCAAATGTTAGAATCTTAGGCATCCTATTTGTTCATGCCATGG GTATTTGCTTTGGACTTGGAGTCTGTACTTTGAAAGAGGCCTTTGAAAAACAAATAATTCTGTGTGAATT TTCTTGTAGCGTGCTTCATGAAAATATCTACTTATCCAGGTTTGCAAATGTACATGTTCATTTGAATGTA AATCACCATTTCTTGGAACCCCACGTTTTTTCTTAAAAATTATTCTGAATTAAATGTATATTTCTTTAGC CTTCCCTACACAGTACTAATAAAAGACTTTTCTTTCTGTTCATGAAAAAAAAAAAAAAAAAAAAAAAGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AJ420555 unedited NAACATGTCAGAATTGTATACAACTCATATAGGCGGCCGCGNAATTCGCACCAGAGAGAA CAGTCTCGAGTTTTTTTTTTTTTTTTTTTTTGCCAGGGCTATGGAGTGGGGGTTGTTTGT CAAACTGATTTTCAATAATTGGATTTAATTTTTTTTAACATTGAAAAGTGCCTGAAAAAT GGTAAATTCTTAAATGTGTGTGAGATTGTCAGAATCAACAAAACTAGGTTGGTTAAACAT ATCTCTGGTACATCAAGGGGCATGATACAAACCAGTCTAAAGACTGTTTATAAAGGAGAG AGCTGGCGACTTATTTTTATTTTTTTTTTTTTTGGACAGAGTCTCCCTTTGTCACCCAAG CCGGAGTGCAGTGGCATGATCATGGTTCACTTCAACCCCTACCTCCTGGGCTCAAGTGTT CCTCTCACCTTATCCTCCTGAGCAGCTGGGACTACAGGCACACACCACCACACCTGGCTA AGTTTTGTATTTTTTGTAGAGATGGGGTTTCTCTGTGTTGCCCAGTCTTGTCTCAAACTC CTGGGCTCAAGCGATCTACCCACCTTGGGCTCCCAAAGTGTTGGGATTACAGGTGTGTGT CACTGTGCCTGACAGCTGACAGTTTTAACTGACAACTTTGATAACAGAGGCTGCTATTTT TGTTTTAGATAATTGGCCAGTGACAGAGTTTACCCTTGCCTCCTTTCTTGGTCTGCCAGC TTTGTCCTTTCTGAGTGATTCTCTTTCTGTATTGAGAGGAAGTGTGGGTCTACATAGGGA TGTTTTGATGCTATGGCAAGAATCCTTTTGTGTTTGGAGTGTAGTCCATTTGGCATAGAA TAAAAAAATCCGTCCCCAATTGTACCTGGGATGTTATAGCCCAGCATCTTGAAATCCTAT GAAATGTTTTAGCACATATCTTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AJ420555 unedited GGCAAACCATGGTCGTCGCCGCACCCTANGATTCGGTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTCAAAAACAAAAAAAAAATTCTTTTATTATTCCTG GGGAGGGAAGGCTAAAAAAATATCCCTTTAATTCAAAATATTTTTTAAAAAAAAACGGGG GGTCCCAAAAAAGGGGGATTTACTTTCAAATGAACATGTCCTTTTGCAAACCGGGATAAT TAAATTTTTTCATGAACCCCCCTCCAAAAAAATTCACCCAAAATTTTTTGTTTTTAAAAG GCCTTTTTCAAAGTACAAACCCCAAGTCCAAAGCAAATCCCCTTGGCATGAACAAATAGG ATGCCTAAAATTCTAACATTTGCTTCGGCATGGTTTTCCTCCTAATTTCCCTACCTCAAA AAAAGAAAAATTTTCCTAAATGGGACAATGGCAAAATATTGGGCTAATACATTTCATAGG ATTTCTAAATGCTGGGCTATAACATCCAGGTTACAATTTGGGGACGGATTTTTTTATTTC TTTTGCAAATGGACTACCCTCCAAACCCAAAAAGATTCTTGCCATAGCATCCAAACATCC CTATGTAAACCCACACTTCCTCTCATTACAAAAAGAGAACTCCTCAAAAAGGACAAAGCT GGCAAACCAAAAAAGGAGGCAAGGGTAAACTCTGTCACTGGCCAATTATCTAAAACAAAA ATAGCAGCCTCTGTTATCAAAGTTGTCAGTTAAAACTGTCAGCTGTCAGGCACAGTGACA CACACCTGTAATCCCAACACTTTGGGACCCCAAGGTGGGTAAATCGCTTGAGCCCAGATT TTGAGACAAGACTGGGCAACACAGAAAAACCCCTCTTTCAAAAATACAAACC
Restriction Sites	NotI-NotI
ACCN	AJ420555
Insert Size	1350 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AJ420555.1
RefSeq Size	1469 bp
RefSeq ORF	1469 bp
Locus ID	255488
Protein Families	Transmembrane
Gene Summary	E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.[UniProtKB/Swiss-Prot Function]

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