PPA2 (BC008246) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC124; SID6-306
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC008246, the custom clone sequence may differ by one or more nucleotides GTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTGCAGGGACCGGGTCGCGCCGTGCTATGGCCCT GTACCACACTGAGGAGCGCGGCCAGCCCTGCTCGCAGAATTACCGCCTCTTCTTTAGTAAGTAGCTCCCG GAGGTTCGCCGAGCGGCGCACCGGAGAAACCTGGGTGGGGGATGGAATGGGGGACACTTGGGACGCCCCG CGCGGTCTCCCTTCTCTCTTTTTGGGGCCCTCAGCGGTAGCCGCGCGGTCCAGGCCACCTTACTTCCCGC GGGAGCGCGCACCGAGTCACGAGAACTGCCAGCGAGGAGGCGGCGGATCAAGACCCCAAAGCGGAGAGCA GAGGCGCAGGGCTGCCGGCTGGGGGGTAGATAGCGCCCTGTGCCCTGCCAGCCGCCCTCCCCGAGGGGTA GGCAGAGTGCAAGCGGCCACCCCGCGGCGGCCTGCAGCCATTAGCCGGCCCGGCCGCCGTTCCCGGTTCA CACAACTCAGTGAGGAAGGGAAGGCAGAGCCCGCCCACACGCCGGCCTTCCTAGAGGCGTAAGAAACCTC CTACTGCGGGAGCTCCCTGACGTCGGAGAGGCAGGCGGAACTTTGCGGATGCCTAATCCAGCGCGTCTTC TTTTTTTGGAGGGACTCTGATGAAGGTTCTTTCCCCAGAAGCTTGACTTGGTACGCACAAGTTTACACCC TGAGTGTGATCCACCATCCAAAGCCTCGTTGGTTCCGGAGCAGCCCCTTTTCCTCCCCAAGCTGAACAAG CCCAGAAGGCTAAGCGACTTCGGGGGTGTCCCTGAGTTGCCGAGCTCAGAGAAAGGACCCAGGTCTTCGT GACTCATTGTTCCGTGTACCCCATTGTTTCACTGTGTACGTTAACCGTAACAGAAAATAGCGATGTCGGT CGCTTGCCTTCACTCCCCACTGACTGCAGAGCCTTGGAGTGGTAGTATCAAAGTGGGCCTTGCATTATCG CTCCCCTTTTCGGAGCACTGGCTCTGGAGGCTTTCAGTTCTAAGCAGGCCCTCGAGTTCTTCACGTCTTA ACTCTGTGACCCCGGACGAGTTTAAGTTGCTAAGCCTTGTTTTTTCTCATCCGTAAAATAGGGCTGCAAG TATTACATGACATTATATATGTAAAGTGATTAGTCACAGGGCCTAAAATATAAATTACTATTGTTAATAC CGATAATCATTATAGATATTTGTAATTTATGAGAGATTTGCAGTTATGCTACATGAGTTTAGGGTCGAGG ATTATTTTGTGGGAAAGAAGAGTTTGCCTAAAGTCGTGTGATTATAATGCAACTAGTACTAGAATTTCTA TTTTCTTCAAAATTGGTTGCAGTTTTCACCCTATGCCACATATTTTCTTCTATCTTTGGTTAAGACTGAT CAGGTGAACAGGAAGTCTCAAATTTCTGAATGGATTATGTTCTAAAAGGTTACCATGTGATGATTAATGG GAATAGTCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008246 unedited NNNGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCGCTGC TGCGGCTGCTGCGCACGGGTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTGCAG GGACCGGGTCGCGCCGTGCTATGGCCCTGTACCACACTGAGGAGCGCGGCCAGCCCTGCT CGCAGAATTACCGCCTCTTCTTTAGTAAGTAGCTCCCGGAGGTTCGCCGAGCGGCGCACC GGAGAAACCTGGGTGGGGGATGGAATGGGGGACACTTGGGACGCCCCGCGCGGTCTCCCT TCTCTCTTTTTGGGGCCCTCAGCGGTAGCCGCGCGGTCCAGGCCACCTTACTTCCCGCGG GAGCGCGCACCGAGTCACGAGAACTGCCAGCGAGGAGGCGGCGGATCAAGACCCCAAAGC GGAGAGCAGAGGCGCAGGGCTGCCGGCTGGGGGGTAGATAGCGCCCTGTGCCCTGCCAGC CGCCCTCCCCGAGGGGTAGGCAGAGTGCAAGCGGCCACCCCGCGGCGGCCTGCAGCCATT AGCCGGCCCGGCCGCCGTTCCCGGTTCACACAACTCAGTGAGGAAGGGAAGGCAGAGCCC GCCCACACGCCGGCCTTCCTAGAGGCGTAAGAAACCTCCTACTGCGGGAGCTCCCTGACG TCGGAGAGGCAGGCGGAACTTTGCGGATGCCTAATCCAGCGCGTCTTCTTTTTTTGGNAG GACTCTNGATGAAGTTCTTTCCCCAGAAGCTTGACTTGGTACGCACAAGTTTACACCCTG AGTGTGATCCACCATCCAAAGCCTCGTTGGTTCCGGAGCAGCCCCTTTTTCCTCCCAGCT GAACAGCCCAGAAGCTAGCGACTCGGGGGGGTCCCTGAGTGCCGACTCANAGAAGACCCC AGTCTCTGACTCATGTTCGTGTACCCATG
Restriction Sites	NotI-NotI
ACCN	BC008246
Insert Size	2650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008246.1
RefSeq Size	1493 bp
RefSeq ORF	1493 bp
Locus ID	27068
Protein Pathways	Oxidative phosphorylation
Gene Summary	The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

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