INT11 (CPSF3L) (BC008041) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cleavage and polyadenylation specific factor 3-like; CPSF73L; FLJ13294; FLJ20542; INT11; integrator complex subunit 11; INTS11; OTTHUMP00000044092; RC-68; RC68; related to CPSF subunits 68 kDa
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC008041, the custom clone sequence may differ by one or more nucleotides ATGCCTGAGATCAGAGTCACGCCCTTGGGGGCCGGCCAGGACGTGGGCCGAAGCTGCATCCTGGTCTCCA TTGCGGGCAAGAATGTCATGCTGGACTGTGGAATGCACATGGGCTTCAATGACGACCGACGCTTCCCTGA CTTCTCCTACATCACCCAGAACGGCCGCCTAACAGACTTCCTGGACTGTGTGATCATTAGCCACTTCCAC CTGGACCACTGCGGGGCACTCCCCTACTTCAGCGAGATGGTGGGCTACGACGGCCCCATCTACATGACTC ACCCCACCCAGGCCATCTGCCCCATCTTGCTGGAGGACTACCGCAAGATCGCCGTAGACAAGAAGGGCGA GGCCAACTTCTTCACCTCCCAGATGATCAAAGACTGCATCCTCCTGGAGACCTTCTGGGAGCGCATGAAC CTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAACCACTACTACAAGCTGTTCATCC CCTGGACCAACCAGAAGATCCGCAAGACTTTTGTGCAGAGGAACATGTTTGAGTTCAAGCACATCAAGGC CTTCGACCGGGCTTTTGCTGACAACCCAGGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCT GGGCAGTCCCTGCAGATCTTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACT GCGTGCAGGGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAGGT GCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAGGGCATCATGCAG CTGGTGTCCTCAGAGCAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCG TGCACCTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGCGTCCT GAAGGACCACTGTGTGCAGCACCTCCCGGACGGCTCTGTGACTGTGGAGTCCGTCCTCCTCCAGGCCGCC GCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGGACCTACCAGGACGAGGAGCTGGGGA GCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCCCAGGCCCCCAGCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008041 unedited NGTTCACATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGCGGGCCTC GCGGGTCCGGGAGCGCGGCGGAGACGATGCCTGAGATCAGAGTCACGCCCTTGGGGGCCG GCCAGGACGTGGGCCGAAGCTGCATCCTGGTCTCCATTGCGGGCAAGAATGTCATGCTGG ACTGTGGAATGCACATGGGCTTCAATGACGACCGACGCTTCCCTGACTTCTCCTACATCA CCCAGAACGGCCGCCTAACAGACTTCCTGGACTGTGTGATCATTAGCCACTTCCACCTGG ACCACTGCGGGGCACTCCCCTACTTCAGCGAGATGGTGGGCTACGACGGCCCCATCTACA TGACTCACCCCACCCAGGCCATCTGCCCCATCTTGCTGGAGGACTACCGCAAGATCGCCG TAGACAAGAAGGGCGAGGCCAACTTCTTCACCTCCCAGATGATCAAAGACTGCATGAAGA AGGTGGTGGCTGTCCACCTCCACCAGACGGTCCAGGTAGATGATGAGCTGGAGATCAAGG CCTACTATGCAGGCCACGTGCTGNGGGCAGCCATGTTCCAGATTAAAGTGGGCTCAGAGT CTGTGGTCTACACGGGTGATTATAACATGACCCCAGACCGACACTTANGAGCTGCCTGGA TTGANCAGTGCCGCCCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGTG ACTCCAAGCGCTGCCGGGAGCGAGACTTCCTGAAGAAAGTNCACGAGACCGTGGAGCGTG GTGGGAAAGTGCTGATACCCTGTGTCGCGCTGGGCCGCGCCAGGAGCTCTGCATTCTNCT GGAGACCTTCTGGNAGCGCATGACCNNTGANGTGCCATCTACTTCTNCACGGGCTGACCG AGAGGNCANCACTACTACAGCTGTCATCCCTGNACACCAGAGATCGAGACTTCTGCAAGA A 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC008041 unedited GCGTCTATGNACGCGGCCGCATTCTAAGATCGGTTTTTTTTTTTTTTTTTTGTTCAACTT TTACTGGGAAACTGGCTGGTCTAGGGACCACCTGGCCCTAACCAGGAATAAAGGCAAGAC AGCCTGGAGACCAGTTTGTTTCTTCAGCTGCAAACAGCTGCCTGGGCAGGCAGGTGACAC AAGGCCTCTGTCCCCAGGGATGGGACCTGCAGGGTCTGTTCACCCAGGGCACCCACAGTC CTGAAGTGCAGGCCCAGGGTCTGTCCAGCTGGGAGAGGGCAGAAGTGGCGGCTGGGTGAG TTGCCGGCCTCAGCTGGGGGCCTGGGGGAGGCCCTTCTTCAGCAGAGATGTGAGGAAGCT CCCCAGCTCCTCGTCCTGGTAGGTCCAGGAGACCAGCAGCACCTTGGTGCCTGGGTCCTC AGAAGGGGCGGCGGCCTGGAGGAGGACGGACTCCACAGTCACAGAGCCGTCTGGGAGGTG CTGCACACAGTGGTCCTTCAAGACGCTCTTGAGGTGGCTGTAGACGCGCAATGCCGTCTC CTGCTCCTTGCGTGTGTCATGCAAGTGCACGCGGCAGGTGAAGCGCATCTGGTGCTCAGC CAGACCCAGCTCTTTGNAGGCTTGCTCTGAGGACACCAGCCCGAAGTTGCTGTCCTTCAT GATCAAGGTGCCGTGCNAGAGCCGAGGCTTCCTGGCCTCAGGGAGCAGCCCCTGCGCCAT CTCCCGCTTCAGCAGCCCCAGCGAGATGCCTACGGGGATGCCTGGGGCTTGGGGGCAGCG TCACCGTCTCGCCATTGGCCGCATGTTACAATTTGACCCGAAGCTCCTGCTCGATCTTCT GCTTCAAGGACTCCATCTTCTTGGCCTCGCCATGCACCAGAAGCAAGC
Restriction Sites	NotI-NotI
ACCN	BC008041
Insert Size	2350 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008041.2, AAH08041.1
RefSeq Size	1478 bp
RefSeq ORF	1170 bp
Locus ID	54973
Domains	lactamase_B
Gene Summary	The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]

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