Nectin 3 (NECTIN3) (NM_020742) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASPGX2; AUTSX2; HLNX; HNL4X; NLGN4
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC106234 representing NM_020742. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCGGCCGCGAATTCGGCACCAGAGTACTTGTAAGTGTTTTGGCTGGAATATTCTGCTATAGGAGAAGAC GGACGTTTCGTGGAGACTACTTTGCCAAGAACTACATTCCACCATCAGATATGCAAAAAGAATCACAAA TAGATGTTCTTCAACAAGATGAGCTTGATTCTTACCCAGACAGTGTAAAAAAAGAAAACAAAAATCCAG TGAACAATCTAATACGTAAAGACTATTTAGAAGAGCCTGAAAAAACTCAGTGGAACAATGTAGAAAATC TCAATAGGTTTGAAAGACCAATGGATTATTATGAAGATCTAAAAATGGGAATGAAGTTTGTCAGTGATG AACATTATGATGAAAACGAAGATGACTTAGTTTCACATGTAGATGGTTCCGTAATTTCCAGGAGGGAGT GGTATGTTTAGCAACCACTGAATGTGACTTAACTATGTACAATGTTCATTCACACTAGTTGATCATTTT CAGATTGTTCATACTTTTTCTTGAGGAAGAATAAGCTTTTTCAAGTTGATTTTCAAGCTTACTTTTTAT ATTCTAATCTGACAAATGAAAATGTAAAATCTGAGTTCAGTGTATCTAAGCTGCTTTACAATTTTTTTT CAATGCTGTACTACTGTCTCAAGATTTAAATTTTAATGCAGAGTACTTTATTGGTGTGAGGCACACAGG TAAGAAGAAATGTCAACATTAAATGTATGACTTACTTGGTACAAAAATTTTTTAAAAAGGGAACTACCT TGACATTGTGTATTAAATGTTTACCTAAGACTATAATCTCAAGTATGATGTTTGTTTAACATATACCTC TCAAAATTTATCACCACTCAATGACACTGCATCAAAATTGACTATAAAACTAATTCAAGAAATATTTAT ATATATTTTTTAATATACAAAAAATATTTAGCCTGATGGAATGGCTTTCCTTTTCAAACATTATTTTCT AAGTTTCTATACAAATGAAATCTTTACCTCTGCATATTAATGAGCCTTGCCATAATTACTGTAGAGTGG CTTTTCAAAGATATTTTGTTGCACTAAAACTGTGGTAGTAAACTCAGTGAACATGATGTGTGGAAGAGC ATAATTAGCTGGTCAATATTTTTGTCCAAAATACCTGCAAGAGTAATAAAATACATACCTTTCAAACAT GATAATTATTAGTTTTTTTTTTTCCTTTCTGGAACATGGATTTTGGTACATTAGCAGTAGCCTTATTTT AATGCTTTATGTCCTAAACATACTAATAGAAATGAAAAGACGCAGAGAGAGCATTTCGGAATACTGAAG TACTAGTTTTAGAAATGAGACTTTCAGCCAACAATCTATAGAAAGAATTTTATGGACCATCTTGTTTTA GTTATTTAATGTTGATGTTGTTCAAATGGGTAAATGTACAGAAAGAAAATTTTAGAGTAAACTTGGAAC TTTGGATATAACTAGAAAAAACTAGATTATAGAATTAGTCAGTAACACTTGCTAATGGACATTGGCATT CATCTCCTTTTTCCTCCTAAGTGTATGTATGTGTTTTAAGATTTCTGTTTTTACGATTAAAACTGGAAA CATGAGGTTTTTTGTTTTTGTTTTTTTACATAATTACATATATTCCTTCTGAATCATTTATCTTTTGAG AAAGAAATGTTACCTAAACTTCAAATGTGCTTTTTGTTTGTGAGGTAATTAAATTGCTTCTACAGTGGA GGCTTACAAAATTATTGTGACAACTATTTTGAAGCTGAAAGGATAGTTTTTCTATTGCTAAGTCATTTG AAAAAGTGACCATTTTGCCAGTGAAATGAAGTGGAAGTTAGTAGGAGAATCATAAATTAAATATATTAT TTTGTTAATAAAAAGGCAAAGTAGTAGGTACTTTTTAAACCCTCCCAACCAGCCCTTTCTCAATATTCA TCAAATCTAAAACATTTAGGGGGCAAAATTCTAACATGTTCATGGTATCTTGCAAATAGTGAAAGCTTT ATTCTGAAGGATTATAAACTAGTTTTCTTCATTTTAACTAGCACTATTTTGTGGAAATTAGAAACCTCT TTTATTTCTCTTCCCAAAAGTAATACTTATTATAAGGCTGTAGTATCAGGTTAAGGATACAGATAAATA AAGTTCACTTATATCTTCTTACAAATGTCTGGGTTTTAATATGGTTAATCACTTATATACAAATATTAC AACTTTTTAGTGCAAGTTTTTGGAAGAAAACTTTTTGATAAAACACTGTGATTGATGTGACTTTATTTT TAATTTAAACGATGAGGTGGCCAGAAGAAAGATGGGTCTAAAATTTCTCCCCATGAAAGATGTAAAACT ATGGCTTTTTTTAAAATCAAAATTTCATCTTTTAAAATAATGGTTTGAAATACTGTATGGATCTGAACA GAATAATCACATTTAGGATTCTATATAAATCTCAACTGGAGTATAATCTGAAGGAAATTAGCAGTGTAT TTTAAGAAATATATTTCAAAAATATAAATACTGATTATGAACTTCCTTTTACATTGTGGTTATTTGTGC GATTAGGTTTTTTTGTTTGTTTCTTTTGTGTTTGTTTGGCGGGAGAGGGTGACCTGGAAAGCCACAAGT GAGTATTTGACATATTCTGTATCCTTAATCCAATCATTTGGCAAACTAAAAGGTTTCTGTGTTGTAAGA ATCTGATACTAGTGCTTAAGACTTTGGGAAGCATTGCACTGTTGTTTATTAGAACTTTATGTATATTTA CTGTACATAGAGACTTGTTTGAAAACATGAATAGTCATTAAATAAAGACATTGTTAAATTAAAAAAAAA AAAAAAAACTCGACTCTAGATTGCGGCCGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_020742 unedited NGGGGGGGGGGGGNNNNNNNGGNTCNNCCNNNNTTTATTACACCCGCCCGTTGNCGCAAA GGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGACACTAT AGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGGAGAGGAG GAAAAAAATATATATGTATCTGCAGTACAGGGAGAAGAAGGAGAGAAGCGGCCAGGGCTG GAGATGGTGAAGGCAGGAAGACTTCTGCAAACTGTGAGGCTGCCTTGGAGTTTTCATAAG AAATTGTCCCTGGAGGTGTTGGATGATCACAGCTTCCTTGGAGCATTGCAGTTGCTGGAA TCCAGTTTCAGGATTAAGGGAGGGCTGCCTCCTTGCAATGGGCTGCCAAGAAAACGGCTG TGCTTGTTCTTAACCTCAGGCTCTGTCTGTGATCAGTCTGAGAGTCTCTCCCAGGTCTAC TGCTCCCTGGAAAGCCCTATCTCTCTGCAGGCTCGCCTCTGGGCTTTGTCTCCTTGGAGC CACATCACTGGGACAGCTGTGGATGTGGATGCAGATTTGAACCATGTCACGGCCCCAGGG ACTGCTATGGCTTCCTTTGTTGTTCACCCCGGTCTGCGTCATGTTAAACTCCAATGTCCT CCTGTGGTTAACTGCTCTTGCCATCAAGTTCACCCTCATTGACAGCCAAGCACAGTATCC AGTTGTCAACACAAATTATGGCANAATCCGGGGCCTAAGAACACCGTTACCCAATGAGAT CTTGGGTCCAGTGGAGCAGTACTTAAGGGTCCCCTATGCCTCACCCCCACTGGAGAGAGG CGGTTTCAGCCCCCAAACCCCCGTCCTCCTGGACTGGCATNNCGAATACTACTCAGTTTG CTGCTGTGTGCCCCCAGCACCTGGATGAGAGACCTTACTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_020742 unedited CTATGTACGCGGCCGCATTTAGNATCGAGTTTTTTTTCTTTTTTTTTTCAATTTTTTTTC TTTTTTCTCTTTTTTATAGATAGCAAGTATATTTTTTCTCATAGAACTCTATGAAGATTC TATAACTTCTTTCCATGTAGAAATAAGATATCATTTAAGATGTACTAAAATCACTTTTGA TCATAATCCCCTGTAAAAGCTAAAGTTATTCACTTAACAGGAACTCTGTTTTTCCTTATT CAAATGTCACAAGCCTGACGCGTTACTGTACATATTGCTAGCAGGAGACAACTGGAAATA CTAAACAAATACTGGAATTCACATTACAGACAGACGAAACCAACATGGATGCCACACATA ACTTCCTTTGTAGTTTCACAGAGAGCCTATTTGTGGTTGCTCAGGTGGGGTCATACATTG CTTGCAGAAATGGCCTGATCATAGCTCTATGAAACAATGAATTCGGAATGAAATCTTACC ATGACACCTCTCTGTAGGAAAGAAATGTTGCTTCACGTGTGCTAAGTTGAGATAATAATA TTTCACATATTTATATACAGAGAATCACTCTCAAATTTAACCCAAGATAAGCAATAGGAT TTGGGGGTGACTTGTACACATTTCTAANAACACTTTTCTTTTTTCTAGAGGTCACTCTCA AACACTGATATATCACTATAGTTTGAGTGTAGGGATTCAGTAATCAAAGGTTGTTATTGC ANAAGAGCCCAGCAGCTGGACAATTTTTATGGGGAATGTCCACTTTAGCGNAGAGATTTA AGACTGGATTTCTAGAATAACCCANCATGGAGCCCGAGAAACATGATCTCAATATCTTTG GCTACTGGTNNCTGGNNTATGCAATATTTTTCAAAAAGTATCCATGCTACTATGTTNCCT TNCCANNCTATACACTCAAAACTTTCCTGANATGAGNAGAGAAATGAAAATGACAGCTGT TGG
Restriction Sites	NotI-NotI
ACCN	NM_020742
Insert Size	2859 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017572.1
RefSeq Size	1276 bp
RefSeq ORF	2859 bp
Locus ID	57502
UniProt ID	Q8N0W4
Domains	COesterase
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)
MW	113.7 kDa
Gene Summary	This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 3. Variants 1, 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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