NMNAT2 (AF288395) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C1orf15; PNAT2
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106206 sequence for AF288395 edited (data generated by NextGen Sequencing) ATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGCCTGCGGCAGCTTCAATCCCATC ACCAAAGGGCACATTCAGATGTTTGAAAGAGCCAGGGATTATCTGCACAAAACTGGAAGG TTTATTGTGATTGGCGGGATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTC GTGTCAAGCCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGATC AGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGCAGCGTGTTGGAA CACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCATCCTCTCCAATGTCAACACACCT TCCATGACACCTGTGATCGGACAGCCACAAAACGAGACCCCCCAGCCCATTTACCAGAAC AGCAACGTGGCCACCAAGCCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTC AGCCGGATCTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATGCC AATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTGCTGCTGTGTGGT AGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTGGAACGAGGCAGATATGGAGGTG ATTGTTGGTGACTTTGGGATTGTGGTGGTGCCCCGGGATGCAGCCGACACAGACCGAATC ATGAATCACTCCTCAATACTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGAC ATCAACCATCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATGGG GACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATCCTCAAAAGCCAG CTGTACATCAATGCCTCCGGCTAG Clone variation with respect to AF288395.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF288395 unedited NNTTCACTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG CCGCGAATTCGGCACGAGGCACCGCCGGAGCCTCGCCTTCCTTTCTCCCTCTGCAGACAC AACGAGACACAAAAAGAGAGGCAACCCCTAGACCACCGCGAAGGACCCATCTGCACCATG ACCGAGACCACCAAGACCCACGTTATCTTGCTCGCCTGCGGCAGCTTCAATCCCATCACC AAAGGGCACATTCAGATGTTTGAAAGAGCCAGGGATTATCTGCACAAAACTGGAAGGTTT ATTGTGATTGGCGGGATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTG TCAAGCCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGATCAGG GTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGCAGCGTGTTGGAACAC CACCGGGACCTCATGAAGAGGGTGACTGGCTGCATCCTCTCCAATGTCAACACACCTTCC ATGACACCTGTGATCGGACAGCCACAAAACGAGACCCCCCAGCCCATTTACCAGAACAGC AACGTGGCCACCAAGCCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGC CGGATCTGCTGTGTCCGCCCGCCCGTGGAGCGTTTCACCTTTGTAGATGAGAATGCCAAT CTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTGCTGCTGTGTGGTAGT GACCTGCTGGAAGTCTTCTGCATCCCAGGGCTCTGGGAACGAGCAGATAGGGAGGTGATT GC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF288395 unedited NGGCCCTACTATTACCGTGCCGCTTTCTAGATCGATTTTTTTTTTTTTTTTTTCTTGTAG CATTGTCTTATTTATTAACATAATTGAAACATTTTGCATAAAACTCTTGCCCATGACTAT TCTAGCAACAAAATTGTACTCAAAATATTTCACTGTGAAATGGTATTGCAACCTTGAATA TCATTTTTTATTAATGAATTGATTTCCATAAAGCAAATCTTACTCTTAAAATGGCAGATT ATGTGATCAAAAAGCGATTCAAAAAAGCTTCCCCCTCCTCATGCCAACCCTCAAGACCAT GTGGATCCAGCTGAATCCTCACCCTGGGACTAGACTAAGTGAGGAAAACCGTACTCTTCC TAACCGACAGCTAATAGACCTCCAACTCCACTACAAAACCCTGACATCATGTGCTGGGCA CTATAATGGGCAACGGATGAATGCAACTATTCTCACCTTCTACTGCTTTCTAAGGCCATT GTTGACACAATCGCCCTGTTCATCTTCCATCTACTGTTGCCCTCTGCATAGTACCTGGAG AATCCGACTTTTCATATTCTACCGTTTATTTTTGCATTACTGTTTGGATGAGTAAAATGT AATAACATGCTTCTCTCTCTTAATCCCCTAAAAAATGTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTGTNNNNCCAATTTTGAGGAGAGTAGAAATTGGGCTGGTTAGTACAAATTTTT TTCTCCCCCGAAATTCTCTGCCCTTTGCCTGAGTTCAGTTATTATTGAAGCCCACTTTCC ACCTAAT
Restriction Sites	NotI-NotI
ACCN	AF288395
Insert Size	5500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF288395.1, AAG60615.1
RefSeq Size	5691 bp
RefSeq ORF	5691 bp
Locus ID	23057
Domains	CTP_transf_2
Protein Pathways	Metabolic pathways, Nicotinate and nicotinamide metabolism
Gene Summary	This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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