Adipose Triglyceride Lipase (PNPLA2) (BC017280) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	1110001C14Rik; adipose triglyceride lipase; ATGL; DESNUTRIN; DKFZp667M109; FP17548; patatin-like phospholipase domain containing 2; PEDF-R; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; TTS-2.2; TTS2
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017280, the custom clone sequence may differ by one or more nucleotides ATGTTTCCCCGCGAGAAGACGTGGAACATCTCGTTCGCGGGCTGCGGCTTCCTCGGCGTCTACTACGTCG GCGTGGCCTCCTGCCTCCGCGAGCACGCGCCCTTCCTGGTGGCCAACGCCACGCACATCTACGGCGCCTC GGCCGGGGCGCTCACGGCCACGGCGCTGGTCACCGGGGTCTGCCTGGGTGAGGCTGGTGCCAAGTTCATT GAGGTATCTAAAGAGGCCCGGAAGCGGTTCCTGGGCCCCCTGCACCCCTCCTTCAACCTGGTAAAGATCA TCCGCAGTTTCCTGCTGAAGGTCCTGCCTGCTGATAGCCATGAGCATGCCAGTGGGCGCCTGGGCATCTC CCTGACCCGCGTGTCAGACGGCGAGAATGTCATTATATCCCACTTCAACTCCAAGGACGAGCTCATCCAG GCCAATGTCTGCAGCGGTTTCATCCCCGTGTACTGTGGGCTCATCCCTCCCTCCCTCCAGGGGGTGCGCT ACGTGGATGGTGGCATTTCAGACAACCTGCCACTCTATGAGCTTAAGAACACCATCACAGTGTCCCCCTT CTCGGGCGAGAGTGACATCTGTCCGCAGGACAGCTCCACCAACATCCACGAGCTGCGGGTCACCAACACC AGCATCCAGTTCAACCTGCGCAACCTCTACCGCCTCTCCAAGGCCCTCTTCCCGCCGGAGCCCCTGGTGC TGCGAGAGATGTGCAAGCAGGGATACCGGGATGGCCTGCGCTTTCTGCAGCGGAACGGCCTCCTGAACCG GCCCAACCCCTTGCTGGCGTTGCCCCCCGCCCGCCCCCACGGCCCAGAGGACAAGGACCAGGCAGTGGAG AGCGCCCAAGCGGAGGATTACTCGCAGCTGCCGGGAGAAGATCACATCCTGGAGCACCTGCCCGCCCGGC TCAATGAGGCCCTGCTGGAGGCCTGCGTGGAGCCCACGGACCTGCTGACCACCCTCTCCAACATGCTGCC TGTGCGTCTGGCCACGGCCATGATGGTGCCCTACACGCTGCCGCTGGAGAGCGCTCTGTCCTTCACCATC CGCTTGCTGGAGTGGCTGCCCGACGTTCCCGAGGACATCCGGTGGATGAAGGAGCAGACGGGCAGCATCT GCCAGTACCTGGTGATGCGCGCCAAGAGGAAGCTGGGCAGGCACCTGCCCTCCAGGCTGCCGGAGCAGGT GGAGCTGCGCCGCGTCCAGTCGCTGCCGTCCGTGCCGCTGTCCTGCGCCGCCTACAGAGAGGCACTGCCC GGCTGGATGCGCAACAACCTCTCGCTGGGGGACGCGCTGGCCAAGTGGGAGGAGTGCCAGCGCCAGCTGC TGCTCGGCCTCTTCTGCACCAACGTGGCCTTCCCGCCCGAAGCTCTGCGCATGCGCGCACCCGCCGACCC GGCTCCCGCCCCCGCGGACCCAGCATCCCCGCAGCACCAGCTGGCCGGGCCTGCCCCCTTGCTGAGCACC CCTGCTCCCGAGGCCCGGCCCGTGATCGGGGCCCTGGGGCTGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017280 unedited CCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGGTCTATATAAGCAGAG CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT CGGCAGAGGGGGGACCCCGAGCTAGAGCCGCAGCGGGACCTGCCCGGCCCCCGGCTCCAG CGAGCGAGCGGCGAGCAGGCGGCTCACAGAGGCCTGGCCGCCCACGGAACCCGGGGCCCG GCGGCCGCCGCCGCGATGTTTCCCCGCGAGAAGACGTGGAACATCTCGTTCGCGGGCTGC GGCTTCCTCGGCGTCTACTACGTCGGCGTGGCCTCCTGCCTCCGCGAGCACGCGCCCTTC CTGGTGGCCAACGCCACGCACATCTACGGCGCCTCGGCCGGGGCGCTCACGGCCACGGCG CTGGTCACCGGGGTCTGCCTGGGTGAGGCTGGTGCCAAGTTCATTGAGGTATCTAAAGAG GCCCGGAAGCGGTTCCTGGGCCCCCTGCACCCCTCCTTCAACCTGGTAAAGATCATCCGC AGTTTCCTGCTGAAGGTCCTGCCTGCTGATAGCCATGAGCATGCCAGTGGGCGCCTGGGC ATCTCCCTGACCCCGCGTGTCAGACGGCGAGAATGTCATTATATCCCACTTCAACTCCAG GACGAGCTCATCCAGGCCAATGTCTGCAGCGGTTTCATCCCCGTGTACTGTGGGCTCATC CCTCCCTCCTCCACGGGGTGCGCTACGTGCATGGTGCCATTTCAGACACCTGCCACTCTA TGAGCTTAAGACACCATCACAGTGTCCCCCTTCTCGGGCGAGAGTGACATCTGGTCCGCA GGAAGGTTCCCCAACATTCACGAGCTGCGGGGTTACAACACCAGCATTCAGTTTAACTTG GGGCACCCTCTACCGGCTCTTCCAAGGCCCNTTTCCCGNCGAGACCCTGGTGCTGTNAAG AGAAGTGCAACCCGGAACCCCGGATGCCCTGCGCTTCTGCG
Restriction Sites	NotI-NotI
ACCN	BC017280
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017280.2, AAH17280.1
RefSeq Size	2113 bp
RefSeq ORF	1515 bp
Locus ID	57104
Domains	Patatin
Protein Families	Transmembrane
Gene Summary	This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.