ASB8 (AF130079) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PRO2852
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF130079, the custom clone sequence may differ by one or more nucleotides ATGCGGGAGTGCAATGGCGTGATCTTGGCTCACTGCAGCCTCCGCCTCCTGGGTTCAAGCAATTCTCCTG TCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCCTGCCACCACGCCCAGCTAATTTCTGTATTTTTAGT AGAGACAGGGTTTCATCATATTGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCACCCGCCTGG GCTTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCTGGTTTGTGCTTGTTGTATCAAAT TTGGGAAATCCTTCTTTGCTGCCAGGCATGTTGTTTTAATAAATACAGTAGTATACCTATCTATAATGTG GTATATCTCATCTGACATTTATTTCCAATATTTGCTTGATTACAAAGGCAGACTTCTTAGAAGTTGCTTT CTTATTATCTTTGGAGTTATAGGGCCACAGGGTTTTAAAAGGAGGTTGGATTGTGCCTTACATGTGGGTT TCATACATTGTCCAAACTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF130079 unedited TCTATTCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGC GAATTCGGCACCAGGCTGACAGAACGCATCTCTGTCTCAAAAAAAGAAAAACAAACAAAC AAACACATTCAGTTCTCACACTTGAATTAGGTAGGTAGGTTGTGTTATGCGTGCTTTTTA TGTATTGAGGAAACAGGATCACAGAGGTTGTGTAACTTAACTAAGGTTACAACTAGGTAG ACTGAATTTGAACTGAAATCTGTCCAACCTCAATGCCTATGCTGCATTCTTTTATGCCAT TTTGCCCCTCTTAGGTGGTCACAGGACCATTCCAATTCCTATAAGGTATAAGCACTAGTT TTAAAGTAATACCATTGTGAACTGCTTGTTCTCATTGAAATAATAGTGTAACTGGAATGA TAAATAGTTCATTAAAATTTTAGTCACATTATGTCATCAGAATTCTAGGAGGAAATGAAT ACCTGATTATTTTGTACTTCACAAAATAAACTGGATAAACTGGATAAATGACTGAAAGTT TCTTTTTTATATTGCTGAGTCAGATTGGATCCGGTTCATTAAGGGCCAAAATTTGTATTT GGCAGTAATCCTCATGTGTTCTCAGAATGCTTGATTGGTCATTATCAGTTGCTTAATGCT TACCAAACACTTCCAGTGTTTTGAGTCACTGAATTGGCTCATGGTAATTGTTAATCTTGT GGTTACTGAGTCAAGAGCCAGCAGTGTGTGTTTTGGCTGCTTCACTTCCTCCCTCACACT TGATATTGTCAGATTTTAAATTAATTTTTTTTTTCTAATCTGGTGGGGTTTGAAATGTAT CTGTAGTGTTTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF130079 unedited GGAAATCTATGTCCGCGGCCGCAATTTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAG TGAACCCAATTACTCAGATGTTTTATATTTCAGTGTGATCCTCGCCCAGATCAAATACTT GACTCCTTAGAAGAATAATTCCTAAAGTCAAAGTAATCACTAGTCTTCTCATTGAAAAAA AGAAAAAATGTTTTTTAGCAGCTTTAATACACAACATAGAATAATCAATGCCACCAAGAT GTATATATGTATTTATACATATACCTTAAGTCATAAGGGTTTAATGGTAGATAGGAGACA CACAAGTCCCAGGAACTGGACAGTTTTGTTTTTTTTTTTAAAAAAAGCAAAAAACATTCT TCTAAGAAGGTTCCCTATCAACTCTTTCACAATCTACCATTATTCTTGGTAAGATAGGGG ACCCCCCAGTCCCAGGGACCCGACCGAGTTTAAAAAAAAAAAGCAAATACATTCCCTCTG TTTCCATATCAGCTTTTTCACAATCCACAACTTTTTCTGGTTAAGCCCCACACTGGTAAA ACTAGACATCAAAAAAATTTTGATATTTTTAGTGGAAGTGCTAACAGCTTAGACTGAATG ACACCCTATCAATTGATATAATACTGGTCACACAAGGAAGGTCTTANTGTCAATCACATT AAAATTTTAATANGATGCACATCTTATTCACCAAATCTGGTTGGTGTCTCANTTTGGAAC AATGTATGAAACCCACCATGTTAAAGCCCAATCCCACCCCCCTTTTAAAACCCCTGTGGC CCTATTAACTTCCAAGATAATTAAGAAAGCAAACTTCTAAAAAGTCCGCCCTTGGTAATC AGCCAATTTTGGGAAATAAATGTCCGAATGAGGAATACCCCCTTTTTGGATAGGGTTTAC TACCGGGTTTATTTAAAACAACCTGCC
Restriction Sites	NotI-NotI
ACCN	AF130079
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF130079.1, AAG35505.1
RefSeq Size	1769 bp
RefSeq ORF	510 bp
Locus ID	140461
Protein Families	Druggable Genome
Gene Summary	May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]

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