Thrombin Receptor (F2R) (BC016059) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CF2R; HTR; PAR-1; PAR1; TR
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC016059, the custom clone sequence may differ by one or more nucleotides CTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGG GGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCG CCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGTCACGTTTAGGTG GGGCGTGCCACCCCCTTCGCGGGCCCAGCCGATGCCCCTTTGGACTCGATCTTGGAGGGTGCAGCCCGCC TGCCACGGGGTGTTGGATATGGAGGAGGATGGAGCGGAAGCCCCCTGGGGGAGCCTGCAGTCCTGCGTTG CACTTGTCATTGTGTTTCTCCCAGGACCACCCCCAAAAAGAAAAGCTCTCACGTTGCTCCACCAATAAAC GTTTCGATCTTTAAAATATAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016059 unedited GGTTCGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTTGG ACCCTGATCTTACCCGTGGGCACCCTGCGCTCTGCCTGCCGCGAAGACCGGCTCCCCGAC CCGCAGAAGTCAGGAGAGAGGGTGAAGCGGAGCAGCCCGAGGCGGGGCAGCCTCCCGGAG CAGCGCCGCGCAGAGCCCGGGACAATGGGGCCGCGGCGGCTGCTGCTGGTGGCCGCCTGC TTCAGTCTGTGCGGCCCGCTGTTGTCTGCCCGCACCCGGGCCCGCAGGCCAGAATCAAAA GCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACCCCAATGATAAATAT GAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTGAATACAGATTAGTC TCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCATCTCAGAAGATGCC TCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTGTGTACACCGGAGTG TTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCATCCTGAAAATGAAG GTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAGATGTGCTGTTTGTG TCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATTGGCAGTTTGGGTCT GAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGGTACATGTACGCCTCTATCTTGCTC ATGACAGTCATAAGCATTGACCGGNTTCTGGCTGTGGTGTATCCCATGCAGTCCCTCTCC TGGCGTACTCTGGGAAGGGGCTNCNTCACTNGTCTGGCCATCTGGGCTTTGCCATCGCAG GGGTAGTGCCCTCTGCTCTCAGGAGCAACCATNNCAGTGCCGGNCTCACAT
Restriction Sites	NotI-NotI
ACCN	BC016059
Insert Size	3800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016059.1
RefSeq Size	496 bp
RefSeq ORF	496 bp
Locus ID	2149
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Calcium signaling pathway, Complement and coagulation cascades, Endocytosis, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
Gene Summary	Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

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