CCDC109B (MCUB) (AL832311) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MCUb
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832311, the custom clone sequence may differ by one or more nucleotides GCAGCCCCTTGAACAGGTGAAAGCTGGAATAGAAGCTCATTCGGAAGCCAAAACCAGTGGACTCCTGTGG GCTGGATTGGCACTGCTGTCCATTCAGGGTGGGGCACTGGCCTGGCTCACGTGGTGGGTGTACTCCTGGG ATATCATGGAGCCAGTTACATACTTCATCACATTTGCAAATTCTATGGTCTTTTTTGCATACTTTATAGT CACTCGACAGGTGAGTAGTTTGTTAGGAAAATGGTAAGTTAGAACATCTTTGCAAAGAATGTCTTATCTA AGCAATGAGCAAAAAAGCCTTTTTATTTACTGAATTACTGCCATCTGGTTTTTATGAGCTGGTAAATTTT TTATTGTTTTTCACAAGACTGAATGAAGACTCACAACACAGAGAAATTATATATAACTTCTTTGGAGAAG TAATTAGTGTAACACACAAATTCCTCCATTGGGATAAATATGTGTTCTCTGTGCCCTCATTTATAAAAAA AAACTGAATTTGCATTGCAAAACATTCAGTAACATCAGCTTTGCCTCATGGTTATTTAATATGCCAAAAA TCTCCCTTTTCTTATTTCTTCCTTCTTCTTTCCCATTATGTGTAATTTCTCTTCCCAGCTTGCCTGTTTT TGAAAGGAGCAGCAGATCTTAAATGAGACTAAATGTTTTGTGTTGGCTTATGCTTACTCTCATGGCCATT GCAAATATTTCACTCATTCATCTGCCTTCTTTTGCTTTGGTGTTGCTGAGTCAAGTATCTTTCTTGCAGA GGCATTATGTTCATTCAAAACATGTTGTTTTCTTCTCTCTCTCTTTTTTTTTTAAGGATTATACTTACTC AGCTGTTAAGAGTAGGCAATTTCTTCAGTTCTTCCACAAGAAATCAAAGCAACAGCACTTTGATGTGCAG CAATACAACAAGTTAAAAGAAGACCTTGCTAAGGCTAAAGAATCCCTGAAACAGGCGCGTCATTCTCTCT GTTTGCAAATGCAAGTAGAAGAACTCAATGAAAAGAATTAATCTTACAGTTTTAAATGTCGTCAGATTTT CCATTATGTATTGATTTTGCAACTTAGGATGTTTTTGAGTCCCATGGTTCATTTTGATTGTTTAATCTTT GTTATTAAATTCTTGTAAAACAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832311 unedited GGTGTGCAAATTAGGTAATACGACTCATATAGGCGGCNCGCGATTCGGCACGAGGGTGTT CTCTGTGCCCTCATTTATCCCAATGGCAGAATTTGCATTGCAAAACATTCAGTAACATCA GCTTTGCCTCATGGTTATTTAATATGCCAAAAATCTCCCTTTTCTTATTTCTTCCTTCTT CTTTCCCATTATGTGTAATTTCTCTTCCCAGCTTGCCTGTTTTTGAAAGGAGCAGCAGAT CTTAAATGAGACTAAATGTTTTGTGTTGGCTTATGCTTACTCTCATGGCCATTGCAAATA TTTCACTCATTCATCTGCCTTCTTTTGCTTTGGTGTTGCTGAGTCAAGTATCTTTCTTGC AGCGGCATTATGTTCATTCAAAACATGTTGTTTTCTTCTCTCTCTCTTTTTTTTTTAAGG ATTATACTTACTCATCTGTTAAGAGTAGGCAATTTCCTTCAGTTCTTCCACAAGAAATCA AAGCAACAGCACTTTGACGTGCAGCAATACAACATGTTAAAAGAAGACCTTGCTAAGGTA TACTACAAATACATCCTATAGCCGGTTTGTTTGGGAGCCAGAACTTACTACCAGGGAAGC ATAACTCGGGGCTCATCCTTACCAAATTAATCTTGGTCCTATATTTCTGTGTGTTGTGGG GATTGCTCATTGAAATGGCTCGCCATTTTAATATTAATCCCCTCTCCCACTTCTACTCTT CTGTCCACCAAAATAGCTTTAAAATGCTGCGCTAAAATTCCCAAATGACCTTAAATACTC AAATAAAATTTTACCTGGAAATCCTTTCTAAACAATATTAAAAAACCCCTTTAAGAAGCG AAACCTGTATAAAGAACCTCACGCCTTTTAAAGTCTTTGCCCCCAGATCAAAAACATTTT CCTTCCCCCCCTCAATCTTTTC
Restriction Sites	NotI-NotI
ACCN	AL832311
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL832311.1
RefSeq Size	1158 bp
RefSeq ORF	1158 bp
Locus ID	55013
Protein Families	Transmembrane
Gene Summary	Negatively regulates the activity of MCU, the mitochondrial inner membrane calcium uniporter, and thereby modulates calcium uptake into the mitochondrion. Does not form functional calcium channels by itself. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways.[UniProtKB/Swiss-Prot Function]

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