ZC3H13 (BC019000) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp434D1812; FLJ35669; KIAA0853; OTTHUMP00000018357; zinc finger CCCH-type containing 13
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC019000, the custom clone sequence may differ by one or more nucleotides CTCCTAGCGGCCTGGTAGTGTTTTTGTTGCCTTTTCTTAATCTACAATCTCTTCGTTATTTTTCTTCCTG CGACCCAGTTTCGCTTGACCCTGGAGAGGCGGCGGGCGGGTTGGTTCTGCTTCTCAGCCATCCCGGGGGC TCCTCGCTAGCCAAGAGCCGGTTCCCGGGAGCCGCGCGCGCATCGCTTTCTCCTCGTCGTCGTCCTCCTG GGTCCAGGCGCGGGGACAGAGTCGCCTCCCCCGCTCCTCGGAGCGGCGGCGGCGGTGGTGCCTCCGGACT GCACTTGCGAAGGGAGCTTGGGGAGGAATACAAAATGTCAAAAATTAGAAGGAAGGTCACAGTGGAAAAT ACCAAGACTATATCTGATAGCACATCCCGAAGACCCAGTGTATTTGAGAGGCTTGGACCCAGCACTGGCA GTACAGCAGAGACACAGTGCCGTAACTGGCTGAAGACTGGCAACTGCCTCTATGGAAACACATGTAGATT CGTACATGGCCCTTCACCTCGTGGTAAAGGTTATAGCAGCAATTATAGAAGGTCACCAGAAAGACCTACA GGGGATCTTAGAGAAAGAATGAAGAACAAGCGCCAAGACGTGGACACTGAGCCCCAGAAACGAAATACAG AGGAGTCATCCTCACCTGTTAGGAAAGAATCTTCAAGAGGGAGACATAGGGAAAAGGAAGACATAAAAAT CACTAAGGAAAGAACTCCAGAAAGTGAAGAAGAAAATGTAGAATGGGAAACTAATAGAGATGATTCTGAC AATGGAGATATTAATTATGATTATGTTCATGAATTGTCATTGGAAATGAAGCGTCAGAAGATACAGAGGG AATTAATGAAGCTGGAACAAGAAAACATGGAGAAGAGAGAAGAAATTATCATTAAAAAGGAGGTTTCACC AGAAGTGGTTAGATCAAAATTGTCCCCGTCACCTTCTCTAAGAAAGTCTAGCAAATCTCCGAAGCGAAAA TCAAGCCCGAAGTCGTCTTCAGCTAGCAAGAAAGATAGGAAGACATCTGCAGTATCTTCTCCCCTGTTGG ACCAGCAGAGAAATTCAAAAACCAACCAAAGTAAAAAGAAAGGACCACGTACTCCTAGTCCACCCCCTCC TATACCAGAAGATATCGCTCTGGGGAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC019000 unedited GCGGCCGCGAATTCGGCACGAGGGCGGCCTGGTAGTGTTTTTGTTGCCTTTTCTTAATCT ACAATCTCTTCGTTATTTTTCTTCCTGCGACCCAGTTTCGCTTGACCCTGGAGAGGCGGC GGGCGGGTTGGTTCTGCTTCTCAGCCATCCCGGGGGCTCCTCGCTAGCCAAGAGCCGGTT CCCGGGAGCCGCGCGCGCATCGCTTTCTCCTCGTCGTCGTCCTCCTGGGTCCAGGCGCGG GGACAGAGTCGCCTCCCCCGCTCCTCGGAGCGGCGGCGGCGGTGGTGCCTCCGGACTGCA CTTGCGAAGGGAGCTTGGGGAGGAATAGTACAAAATGTCAAAAATTAGAAGGAAGGTCAC AGTGGAAAATACCAAGACTATATCTGATAGCACATCCCGAAGACCCAGTGTATTTGAGAG GCTTGGACCCAGCACTGGCAGTACAGCAGAGACACAGTGCCGTAACTGGCTGAAGACTGG CAACTGCCTCTATGGAAACACATGTAGATTCGTACATGGCCCTTCACCTCGTGGTAAAGG TTATAGCAGCAATTATAGAAGGTCACCAGAAAGACCTACAGGGGATCTTAGAGAAAGAAT GAAGAACAAGCGCCAAGACGTGGACACTGAGCCCCAGAACGAAATCAGGAGGAGTCATCC TCACCTGTTAGGAANAGAAATCTTCAAGAGGGAGACATANGGGAAAAGGAAGACATANNA ATCACTAAGGAAAGAAACTCCAGAAGTGAAGAAAGAANATGTAGAATGGGAAACTNATAG AGATGATTCTGACATGGAGATATAANTTGGAATTATGGTCATGAATTGTCATTGGAAATG AAGCGTCAGAGATACAGAGGGAATATTGGAGCTGGACAAGAAACTGGGAGAAGAGAGAAG AATATCATANAAAGGGAGGGTCACCANAGTA
Restriction Sites	NotI-NotI
ACCN	BC019000
Insert Size	4400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC019000.1
RefSeq Size	1172 bp
RefSeq ORF	1104 bp
Locus ID	23091
Domains	zf-CCCH
Gene Summary	Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3' UTR (By similarity). Controls embryonic stem cells (ESCs) pluripotency via its role in m6A methylation (By similarity). In the WMM complex, anchors component of the MACOM subcomplex in the nucleus (By similarity). Also required for bridging WTAP to the RNA-binding component RBM15 (RBM15 or RBM15B) (By similarity).[UniProtKB/Swiss-Prot Function]

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